4 research outputs found
The control system for radiation sterilization of health-care products at the accelerator LU-10
The regulatory documents for the technological process of sterilization (DSTU ISO 11 137:2003 and others) set
requirements on monitoring, maintaining and archiving the basic parameters of the process. To meet the requirements,
an automated control system was created at the LU-10. It includes a PC-controlled beam scanner, technological
measuring channels (TMC), and also a set of working standards to calibrate the channels. The CAMAC standard
was used as an interface. The software of the complex provides the real-time processing of information on the beam
parameters, their control, archiving, and also the information exchange within the local-area technological network.
The presence of the Customer Database comprising the characteristics of incoming products makes it possible to optimize
the process conditions for the products by the computer simulation method.ΠΠΎΡΠΌΠ°ΡΠΈΠ²Π½ΡΠ΅ Π΄ΠΎΠΊΡΠΌΠ΅Π½ΡΡ Π½Π° ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΏΡΠΎΡΠ΅ΡΡ ΡΡΠ΅ΡΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ (ΠΠ‘Π’Π£ ISO 11 137:2003 ΠΈ Π΄Ρ.) ΡΡΡΠ°Π½Π°Π²Π»ΠΈΠ²Π°ΡΡ ΡΡΠ΅Π±ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠΎΠ½ΠΈΡΠΎΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ, ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΈ Π°ΡΡ
ΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΠΏΡΠΎΡΠ΅ΡΡΠ°. ΠΠ»Ρ ΡΠ΄ΠΎΠ²Π»Π΅ΡΠ²ΠΎΡΠ΅Π½ΠΈΡ ΡΡΠΈΠΌ ΡΡΠ΅Π±ΠΎΠ²Π°Π½ΠΈΡΠΌ Π½Π° ΠΠ£-10 ΡΠΎΠ·Π΄Π°Π½Π° Π°Π²ΡΠΎΠΌΠ°ΡΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½Π°Ρ ΡΠΈΡΡΠ΅ΠΌΠ° ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ. Π Π΅Π΅ ΡΠΎΡΡΠ°Π² Π²Ρ
ΠΎΠ΄ΠΈΡ ΡΠΏΡΠ°Π²Π»ΡΠ΅ΠΌΡΠΉ Π Π‘ ΡΠΊΠ°Π½Π΅Ρ ΠΏΡΡΠΊΠ°, ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ·ΠΌΠ΅ΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΊΠ°Π½Π°Π»Ρ, Π° ΡΠ°ΠΊΠΆΠ΅ Π½Π°Π±ΠΎΡ ΡΠ°Π±ΠΎΡΠΈΡ
ΡΡΠ°Π»ΠΎΠ½ΠΎΠ² Π΄Π»Ρ ΠΊΠ°Π»ΠΈΠ±ΡΠΎΠ²ΠΊΠΈ ΠΊΠ°Π½Π°Π»ΠΎΠ². Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΈΠ½ΡΠ΅ΡΡΠ΅ΠΉΡΠ° ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ ΡΡΠ°Π½Π΄Π°ΡΡ ΠΠΠΠΠ. ΠΡΠΎΠ³ΡΠ°ΠΌΠΌΠ½ΠΎΠ΅ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° Π΄Π°Π΅Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡ Π² ΡΠ΅ΠΆΠΈΠΌΠ΅ ΡΠ΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΠΈΡΡ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΡ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ ΠΎ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠ°Ρ
ΠΏΡΡΠΊΠ°, ΡΠΏΡΠ°Π²Π»ΡΡΡ ΠΈΠΌΠΈ, ΠΈΡ
Π°ΡΡ
ΠΈΠ²ΠΈΡΠΎΠ²Π°ΡΡ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΎΠ±ΠΌΠ΅Π½ΠΈΠ²Π°ΡΡΡΡ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠ΅ΠΉ Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
Π»ΠΎΠΊΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠΈ. ΠΠ°Π»ΠΈΡΠΈΠ΅ Π±Π°Π·Ρ Π΄Π°Π½Π½ΡΡ
Β«ΠΠ°ΠΊΠ°Π·ΡΠΈΠΊΒ» Ρ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ°ΠΌΠΈ ΠΏΠΎΡΡΡΠΏΠ°ΡΡΠ΅ΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΎΠΏΡΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°ΡΡ ΡΠ΅ΠΆΠΈΠΌ Π΅Π΅ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ.ΠΠΎΡΠΌΠ°ΡΠΈΠ²Π½Ρ Π΄ΠΎΠΊΡΠΌΠ΅Π½ΡΠΈ Π½Π° ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΈΠΉ ΠΏΡΠΎΡΠ΅Ρ ΡΡΠ΅ΡΠΈΠ»ΡΠ·Π°ΡΡΡ (ΠΠ‘Π’Π£ ISO 11 137:2003 ΡΠ° ΡΠ½ΡΡ)
ΡΡΡΠ°Π½ΠΎΠ²Π»ΡΡΡΡ Π²ΠΈΠΌΠΎΠ³ΠΈ ΠΌΠΎΠ½ΡΡΠΎΡΡΠ²Π°Π½Π½Ρ, ΠΏΡΠ΄ΡΡΠΈΠΌΠΊΠΈ Ρ Π°ΡΡ
ΠΈΠ²ΡΠ²Π°Π½Π½Ρ ΠΎΡΠ½ΠΎΠ²Π½ΠΈΡ
ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡΠ² ΠΏΡΠΎΡΠ΅ΡΡ. ΠΠ»Ρ
Π·Π°Π΄ΠΎΠ²ΠΎΠ»Π΅Π½Π½Ρ ΡΠΈΠΌ Π²ΠΈΠΌΠΎΠ³Π°ΠΌ Π½Π° ΠΠ-10 ΡΡΠ²ΠΎΡΠ΅Π½Π° Π°Π²ΡΠΎΠΌΠ°ΡΠΈΠ·ΠΎΠ²Π°Π½Π° ΡΠΈΡΡΠ΅ΠΌΠ° ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ. Π£ ΡΡ ΡΠΊΠ»Π°Π΄ Π²Ρ
ΠΎΠ΄ΠΈΡΡ
ΠΊΠ΅ΡΠΎΠ²Π°Π½ΠΈΠΉ Π Π‘ ΡΠΊΠ°Π½Π΅Ρ ΠΏΡΡΠΊΠ°, ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΡΡΠ½Ρ Π²ΠΈΠΌΡΡΡΠ²Π°Π»ΡΠ½Ρ ΠΊΠ°Π½Π°Π»ΠΈ, Π° ΡΠ°ΠΊΠΎΠΆ Π½Π°Π±ΡΡ ΡΠΎΠ±ΠΎΡΠΈΡ
Π΅ΡΠ°Π»ΠΎΠ½ΡΠ² Π΄Π»Ρ
ΠΊΠ°Π»ΡΠ±ΡΡΠ²Π°Π½Π½Ρ ΠΊΠ°Π½Π°Π»ΡΠ². Π―ΠΊ ΡΠ½ΡΠ΅ΡΡΠ΅ΠΉΡ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π½ΠΈΠΉ ΡΡΠ°Π½Π΄Π°ΡΡ ΠΠΠΠΠ. ΠΡΠΎΠ³ΡΠ°ΠΌΠ½Π΅ Π·Π°Π±Π΅Π·ΠΏΠ΅ΡΠ΅Π½Π½Ρ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡ
Π΄Π°Ρ ΠΌΠΎΠΆΠ»ΠΈΠ²ΡΡΡΡ Ρ ΡΠ΅ΠΆΠΈΠΌΡ ΡΠ΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΡΡ ΡΠΎΠ±ΠΈΡΠΈ ΠΎΠ±ΡΠΎΠ±ΠΊΡ ΡΠ½ΡΠΎΡΠΌΠ°ΡΡΡ ΠΏΡΠΎ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΈ ΠΏΡΡΠΊΠ°, ΠΊΠ΅ΡΡΠ²Π°ΡΠΈ Π½ΠΈΠΌΠΈ,
Π°ΡΡ
ΠΈΠ²ΡΠ²Π°ΡΠΈ ΡΡ
, Π° ΡΠ°ΠΊΠΎΠΆ ΠΎΠ±ΠΌΡΠ½ΡΠ²Π°ΡΠΈΡΡ ΡΠ½ΡΠΎΡΠΌΠ°ΡΡΡΡ Π² ΠΌΠ΅ΠΆΠ°Ρ
Π»ΠΎΠΊΠ°Π»ΡΠ½ΠΎΡ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΎΡ ΠΌΠ΅ΡΠ΅ΠΆΡ. ΠΠ°ΡΠ²Π½ΡΡΡΡ Π±Π°Π·ΠΈ
Π΄Π°Π½ΠΈΡ
Β«ΠΠ°ΠΌΠΎΠ²Π½ΠΈΠΊΒ» Π· Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ°ΠΌΠΈ ΠΏΡΠΎΠ΄ΡΠΊΡΡΡ, ΡΠΎ Π½Π°Π΄Ρ
ΠΎΠ΄ΠΈΡΡ, Π΄ΠΎΠ·Π²ΠΎΠ»ΡΡ ΠΎΠΏΡΠΈΠΌΡΠ·ΡΠ²Π°ΡΠΈ ΡΠ΅ΠΆΠΈΠΌ ΡΡ ΠΎΠ±ΡΠΎΠ±ΠΊΠΈ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΊΠΎΠΌΠΏ'ΡΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΠΌΠΎΠ΄Π΅Π»ΡΠ²Π°Π½Π½Ρ
Sensitive luminescent determination of DNA using the terbium(III)βdifloxacin complex
The interaction of the terbiumβdifloxacin complex (TbβDFX) with DNA has been examined by using UVβvis absorption and luminescence spectroscopy. The TbβDFX complex shows an up to 85-fold enhancement of luminescence intensity upon titration with DNA. The long decay times allow additional detection schemes like time-resolved measurements in microplate readers to enhance sensitivity by off-gating short-lived background luminescence. Optimal conditions are found at equimolar concentrations of Tb3+ and DFX (0.1 or 1 ΞΌM) at pH 7.4. Under these conditions, the luminescence intensity is linearly dependent on the concentration of ds-DNAs and ss-DNA between 1β1500 ng mLβ1 and 4.5β270 ng mLβ1, respectively. The detection limit is 0.5 ng mLβ1 for ds-DNAs and 2 ng mLβ1 for ss-DNA. The mechanism for the luminescence enhancement was also studied