Survey and first report of Acanthamoeba T4 genotype in natural spring water resources in the Black Sea, Turkey

Infection with Acanthamoeba spp. may result in granulomatous amoebic encephalitis and Acanthamoeba keratitis. Water is an important habitat where Acanthamoeba species thrive. Therefore, studying the occurrence of this free-living amoeba in water sources will help understand the infection dynamics. The aim of the study was to survey and report on the presence of Acanthamoeba spp. in water resources from the Ordu and Giresun provinces in Black Sea. Acanthamoeba spp. was found in 1/17 natural spring water samples from Ordu and in 2/18 from Giresun. Acanthamoeba species were not detected in any of the investigated tap water samples. Sequencing of the (SSU) rDNA gene resulted in the identification of haplotype I (Acanthamoeba genotype: 10094684). T4 (8.6%) was the only isolated genotype in both Ordu and Giresun provinces. This is the first report of Acanthamoeba T4 genotype in natural spring water resources in the Black Sea. The occurrence of Acanthamoeba species in natural spring water sources should be considered as a potential risk for human infection, especially to high-risk populations

The Levels of Adenosine Deaminase (ADA) in the Serum of Enterobius Vermicularis Positive Patients

It has been reported that the parasites can make the free oxygen radicals such as superoxide and hydrogen peroxide and they contain enzymes which produce them. The cytokines play role in the regulation of immune response in living beings. The synthesizing of IL-10 is increased in Enterobius vermicularis, too. The adenosine deaminase (ADA) is also an aminohydrolase which plays role in the catabolism of purine nucleotides, inosines and deoxyinosine deamidates the adenosine and deoxyadenosine irreversibly. In the study, the comparison of ADA levels between the serums of the control group and the group of patients with E. vermicularis was aimed. In the study, the experimental group included 40 E. vermicularis positive patients. The average age of the patients is 35.2±5.4, 20 of them are men and 10 of them are women. The average age of 40 healthy Enterobius vermicularis negative people is 40.23±7.01, 31 of them are male and 9 of them are female. A significant decrease between the Enterobius vermicularis positive patients and the control group was detected in the level of ADA (p=0.0001). It can be considered that the ADA level of E. vermicularis positive patients in the study may be decreased because of increasing oxidative stress due to the parasitic infection. [Med-Science 2014; 3(4.000): 1648-54

The incidence of demodex in the Faculty of Science Students and measurement of these Students' knowledge level about demodex.

This study was purposed to unveil prevalence of Demodex in faculty of science students and to measure the level of knowledge about Demodex. The study population consisted of Ordu University Faculty of Science students. Participants were selected by random sampling method. A questionnaire including socio-demographic questions was applied. Standard superficial skin biopsy method was employed. In the study, after student volunteers filled out and signed Patients Informed Form, standardized surface skin biopsy from the face after signed (SYDB) samples were taken and analyzed. In the study, 34 (22.7%) of the subjects were males, 116 (77.3%) were females. The mean age of subjects with the standard deviation was 20.10 ± 2.11. 69 (46%) students were found as Demodex spp. All of the students were 1^st,2^nd,3^(rd )and 4^th year Science Faculty students. The study demonstrated that Demodex spp. is a widespread health problem in the faculty of Science students, but the level of knowledge about the Demodex spp. isnt high enough. [Med-Science 2017; 6(1.000): 30-4

Epidemiology of Blastocystis spp. in primary school students at a central village of Ordu province

Blastocystis spp. is one of the most common intestinal parasites in humans and its pathogenicity is still controversial. The epidemiology of this parasite varies depending on the socio-cultural, socio-economic and geographic circumstances. In this study, Blastocystis spp. was investigated in stool samples of a Primary School students in a Central Village in Ordu province who did not have any intestinal complaints. Before starting work, we were granted permission from National Education Directorate and the School Board. Blastocystis spp. was investigated in Primary School Students stool samples by direct microscopy and Trichrome staining. The stool samples which showed any form of Blastocystis spp. were evaluated as microscopically positive regardless of the number per microscope field. Eighty and 85 of the 165 students participating in working were male and female, respectively. As a result of the microscopic investigation, 45 (27.3%) students were identified as positive. Eighteen of the positive students (40%) were male and 27 (60%) were female. Direct microscopic inspection, sedimentation and permanent staining are routine methods used in diagnosis of Blastocystis spp. This study also evaluated trichrome staining for each stool samples. In our research, this parasite was identified in 27.3% of asymptomatic Primary School students stool samples. This suggests the parasite is relatively common in the region. Blastocystis spp. infects via the fecal-oral route. As a result, we presented recommendations for the enhancing personal hygiene, social education and the provision of water treatment for the students to protect themselves from infection with Blastocystis spp. [Med-Science 2019; 8(1.000): 77-80

Common Demodex spp. identified on the arm and abdominal region of patient monitored for widespread pruritus

Demodex mite is a compulsory human ecto-parasite situated in or near pilo-sebase units. Demodex folliculorum and Demodex brevis are two species found in humans. In this case; there were long-term complaints of severe pruritus (six months), and common erythematous papules on the body. Other physical examination findings, complete blood count, biochemistry, sedimentation and C-reactive protein levels were normal. However, Demodex spp. was detected in standard superficial skin biopsy taken from the patients skin. Demodex should be considered prediagnosis for unexplained and continued pruritus complaints and maculopapular lesions. [Med-Science 2019; 8(1.000): 242-4

Acanthamoeba spp. in river water samples from the Black Sea region, Turkey

The present study aims to investigate the occurrence of free living amoeba (FLA) in water resources (rivers and tap water) in Samsun in the Black Sea. The presence of Acanthamoeba spp. was confirmed in 98 of 192 water samples collected from 32 sites of Samsun province (Samsun centre, Terme, Carsamba, Tekkekoy, Bafra) by PCR. Acanthamoeba spp. were found in 15/36 river samples from Samsun, in 58/90 from Terme, in 12/30 from Carsamba, in 7/18 from Tekkekoy and in 6/18 from Bafra. No Acanthamoeba species were detected in tap water samples. The highest rate in river waters contaminated with Acanthamoeba species was in Terme followed by Samsun centre (41.7%), Carsamba (40%), Tekkekoy (38.9%) and Bafra districts (33.3%), respectively. The result of the subsequent sequence analysis showed Haplotype I (A. triangularis) in 5%, Haplotype II (A. polyphaga) in 29.6%, Haplotype III (Acanthamoeba spp.) in 62% and Haplotype IV (A. lenticulata) in 3%. The most common genotype was Acanthamoeba T4 (Acanthamoeba spp., A. polyphaga, A. triangularis) and T5 genotype was also found in 3%. The T4 genotype is the most common genotype associated with Acanthamoeba keratitis (AK) worldwide; therefore, humans and animals living in the area are at risk after contact with such waters

Investigation of Demodex Spp. prevalence among managers and workers of health hazard bearing and sanitary establishment

Two Demodex species are known to live on people. Demodex folliculorum lives in the openings of hair follicles alone or in groups. D brevis lives in the depths of the sebaceous glands alone. There are different related on the epidemiology of Demodex species have been published. Method: In this study, taking into account that the parasite is transmitted through close contact from person to person, we aimed to evaluate the relation between the presence of Demodex spp with gender and age among the Health hazard bearing and sanitary establishment operators and workers who came for porter examination. Results: For this purpose, 862 male and 215 female patients with a total of 1077 volunteers from the face area of the standard superficial skin biopsy (SSSB) method and studied samples were taken. More than five mites in 1 cm2 density was defined as positive. In investigated samples 37.3% Demodex spp. positivity was detected. Only two were found to be D brevis and the others were D folliculorum. The study revealed statistically significant relationships between the positivity of parasites with the occupational group, age and sex. Conclusion: Since the prevalence of Demodex among healthy individuals without any complaint was found to be 37%, we believe Demodex spp should be investigated in porter examinations of people who have dermatological complaints

Prevalence of Cyclospora cayetanensis and Cryptosporidium spp. children according to some variables

In this study, the prevalence of Cyclospora cayetanensis and Cryptosporidium spp. were researched in children with parasitological investigations requested for a variety of reasons, regardless of immune status. The stool samples of 1057 pediatric patients with parasitological evaluation requested for a variety of reasons within two years were investigated under a microscopy using native-lugol, sedimentation and modified Kinyouns acid-fast stain methods. The mean age of the 1057 pediatric patients participating in the research were 8.07±4.32 years, 11.4% were identified to have coccidian parasites (Cyclospora cayetanensis and Cryptosporidium spp.). Evaluation according to age group found the majority of children positive for Cryptosporidium spp. were in the 6-12 year interval. These parasites were observed more frequently in the spring and summer seasons compared to other seasons. There was a significant correlation between parasite positivity and spring and summer seasons (p [Med-Science 2021; 10(2.000): 338-45

Evaluation of the diagnosis of helicobacter pylori from stomach biopsy samples by staining methods

In this study, it was aimed to retrospectively investigate the presence of H. pylori with Modified Giemsa and Hematoxylin & Eosin in gastric antrum biopsy samples of patients who applied to Ordu University hospital with dyspeptic complaints. Also, cross-sections were stained with Giemsa, Wright's eosin methylene blue and modified Giemsa dyes to compare their effectiveness in diagnosis. The population of the study consisted of 2679 gastric biopsy samples sent to the pathology laboratory between 2014 and 2018. Gastric biopsy samples were screened, and samples stained with Hematoxylin & Eosin, Giemsa and Periodic Acid Schift (PAS) were re-examined for H. pylori In the microscopic examination, the samples were also evaluated in terms of intestinal metaplasia, activation and atrophy. In the study, 37 negative, 31 mildly positive, 31 moderately positive and 31 severely positive samples were randomly selected, in addition to routine staining methods. Selected samples were re-sectioned, stained with Giemsa and Wright's eosin dye, and Mayg Grunwald-Giemsa (MGG) dye, Giemsa and Wright's eosin methods were compared. A total of 2679 patients, 49.15% male and 50.85% female, were included in the study. The mean age of patients aged 17-93 was 50.42±15.32. H. pylori positivity was found to be 46.8% in the study. It was determined in the study that there was a significant association between H. pylori severity and inflammation (p [Med-Science 2023; 12(1.000): 115-21

5-Phenyl thiophene amino phenol derivatives: Synthesis, spectroscopic characterization, computational study and antimicrobial activity

WOS: 000458618500005(((5-phenylthiophen-2-yl) methylene) amino) phenol derivatives were synthezised and characterized by IR and NMR. The density functional theory (DFT) method with 6-311++G (d,p) basis set was used for both calculations of molecular geometries and spectroscopic parameters. Frontier molecular orbitals and molecular electrostatic potential maps for compounds la, lb and lc were obtained. Due to the differences observed in the theoretical spectral parameters, N-H center dot center dot center dot O type intermolecular/intramolecular hydrogen bonds in the molecular structures of compounds were proposed. To determine the nonlinear optical properties of compounds, the mean polarizability (a) and the first hyperpolarizability ((3) obtained as 44.2388 x 10(-24) esu and 373.1267 x 10(-31) esu for compound 1a, 43.8141 x 10(-24) esu and 195.2893 x 10(-31) esu for compound 1b, 36.9489 x 10(-24) esu and 120.73825 x 10(-31) esu for compound 1c, respectively were used. These values indicate that compounds can be suggested as a good nonlinear optical material. In addition, the antimicrobial properties of compounds were investigated by considering antileishmanial and antibacterial activities. The compounds were evaluated against Leishmania infantum promastigots for antileishmanial activity and against five standard bacteria (Escherichia coli, Shigella flexneri, Yersinia enterocolitica, Salmonella typhimurium and Salmonella enterica) for antibacterial activity, by microdilution broth assay with Alamar Blue Dye. Results show that only compound la has high antileishmanial activity and can be evaluated in leishmaniasis treatment after in vivo studies and also considered in pharmacological studies for antibacterial activity. (C) 2019 Published by Elsevier B.V