59 research outputs found
Supplementary data for article: BiheloviÄ, F.; KaradžiÄ, I.; MatoviÄ, R.; SaiÄiÄ, R. Total Synthesis and Biological Evaluation of (-)-Atrop-Abyssomicin C. Organic and Biomolecular Chemistry 2013, 11 (33), 5413ā5424. https://doi.org/10.1039/c3ob40692j
Supplementary material for: [https://doi.org/10.1039/c3ob40692j]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1388
Influence of rhamnolipids, produced by Pseudomonas aeruginosa NCAIM(P), B001380 on Cr(VI) removal capacity in liquid medium
Pseudomonas aeruginosa NCAIM(P), B001380, a propitious bacterial strain isolated from mineral cutting oil was identified to be chromium tolerant and a producer of biosurfactant rhamnolipid (RL) with potential application in heavy metal bioremediation. Culture growth, RL production and Cr(VI) removal capacity of the strain in the presence of 50 mg L-1 (I) and 100 mg L-1 of Cr(VI) (II) were studied. Maximum of RL production were found in the late-stationary phase at 72 h for both Cr(VI)-amended cultures: I (236 mg L-1) and II (160 mg L-1), as well as the maximum of Cr(VI) removal capacity: 70 % (I) and 57 % (II). The amount of Cr in RL preparation II was 22 mg mg-1 determined by flame atomic absorption spectroscopy (FAAS). Appearance of a new band at 914 cm-1 in infrared (IR) spectrum of RL (II) indicated a significant proof for possible coordination of CrO42-ion with RL. The effect of Cr(VI) on monorhamnolipids (RL1) and dirhamnolipids (RL2) distribution and its ratio were studied by electrospray ionization mass spectrometry (ESI-MS). An increase was observed in a RL2/RL1 ratio for II compared to control
Metabolomic study of the biodegradation pathway of sodium-benzoate in pseudomonas aeruginosa san ai
The goal of this study was to analyze the products of benzoate degradation by polyextremophilic, hydrocarbonoclastic Pseudomonas aeruginosa san ai using targeted metabolomic analysis in order to determine the specificity of the metabolic pathway of sodium-benzoate.Poster: [https://cer.ihtm.bg.ac.rs/handle/123456789/5126
Niskomolekulski inhibitori proteaza mikrobnog porekla
We have witnessed an explosion of informations about properties, also mode of action and regulation of proteases from the metalloproteases at the cell surface to the AIDS protease. Protease inhibitors have a great importance in mechanism of proteolysis. One of the latest strategies to combat diseases like AIDS and cancer is to use proteinase inhibitors as drugs. Especially good source of protease inhibitors are microorganisms. A few low-molecular-weight proetease inhibitors of microbial origin, as a potential drugs, was described.BuduÄi da je upotreba inhibitora aminopetidaza u leÄenju SIDE, takoÄe i kancera razliÄitih vrsta, tzv. nova strategija u leÄenju ovih bolesti [18] to je u toku traženje novih inhibitora AP a posebno LAP kao proizvoda metabolizma mikroorganizama, ali i stereoselektivna sinteza jedinjenja koja su im sliÄna [19]. Može se pretpostaviti da Äe sledeÄih godina napredak u biohemiji patoloÅ”kih procesa biti pod velikim uticajem napretka u istraživanju niskomolekulskih inhibitora koje produkuju mikroorganizmi ali i napretka u sintezi analoga ovih jedinjenja
Niskomolekulski inhibitori proteaza mikrobnog porekla
We have witnessed an explosion of informations about properties, also mode of action and regulation of proteases from the metalloproteases at the cell surface to the AIDS protease. Protease inhibitors have a great importance in mechanism of proteolysis. One of the latest strategies to combat diseases like AIDS and cancer is to use proteinase inhibitors as drugs. Especially good source of protease inhibitors are microorganisms. A few low-molecular-weight proetease inhibitors of microbial origin, as a potential drugs, was described.BuduÄi da je upotreba inhibitora aminopetidaza u leÄenju SIDE, takoÄe i kancera razliÄitih vrsta, tzv. nova strategija u leÄenju ovih bolesti [18] to je u toku traženje novih inhibitora AP a posebno LAP kao proizvoda metabolizma mikroorganizama, ali i stereoselektivna sinteza jedinjenja koja su im sliÄna [19]. Može se pretpostaviti da Äe sledeÄih godina napredak u biohemiji patoloÅ”kih procesa biti pod velikim uticajem napretka u istraživanju niskomolekulskih inhibitora koje produkuju mikroorganizmi ali i napretka u sintezi analoga ovih jedinjenja
Izolovanje i delimiÄno karakterisanje proteaze iz Pseudomonas aeruginosa ATCC 27853
Enzymatic characteristics of a protease from a medically important, referent strain of Pseudomonas aeruginosa ATCC 27853 were determined. According to sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE, and gel filtration, it was estimated that the molecular mass of the purified enzyme was about 15 kDa. Other enzymatic properties were found to be: pH optimum 7.1, pH stability between 6.5 and 10; temperature optimum around 60 Ā°C while the enzyme was stable at 60Ā°C for 30 min. Inhibition of the enzyme was observed with metal chelators, such as EDTA and 1,10-phenanthroline, suggesting that the protease is a metalloenzyme. Furthermore, the enzyme contains one mole of zinc ion per mole of enzyme. The protease was stable in the presence of different organic solvents, which enables its potential use for the synthesis of peptides.U ovom radu je okarakterisana ekstracelularna proteaza medicinski znaÄajnog, referentnog soja Pseudomonas aeruginosa ATCC 27853. Molekulska masa preÄiÅ”Äenog enzima odreÄena SDS PAGE i gel filtracijom iznosi oko 15 kDa. OdreÄeni su sledeÄi enzimski parametri: pH optimum 7,1; pH stabilnost u opsegu 6,5-10; temperaturni optimum 60Ā°C, a enzim je stabilan na 60Ā°C 30 min. Na osnovu inhibicije enzima pomoÄu EDTA i 1,10-fenantrolina, utvrÄeno je da proteaza predstavlja metaloenzim. Pokazano da proteaza sadrži 1 mol jona cinka po molu enzima. Proteaza je stabilna u prisustvu razliÄitih organskih rastvaraÄa, Å”to omoguÄava upotrebu za sintezu peptida
Dejstvo rifamicina i hloramfenikola na produkciju antibiotika i proteaza soja Streptomyces hygroscopicus CH-7
Streptomycetes are common soil bacteria that grow in a filamentous form and produced spores. Secondary metabolites, particularly antibiotics, as well as extracellular enzymes produced by them have very significant role in the process of differentiation. The Slreptomyces hygroscopicus CH-7 strain produces polyketide antibiotics and an extracellular proteolytic complex. The aim of this work was to establish the influence of transcription and translation inhibitors on biosynthesis of polyene antibiotic and proteolytic enzymes. The inhibitors were added at inoculation and at specific time intervals of fermentation. The production of hexaene H-85 is susceptible to rifamycin (transcription inhibitor) and chloramphenicol (translation inhibitor). Rifamycin reduces the production and trypsin like protease (TLP) and aminopeptidase (AP). while chloramphenicol acts only on TLP. Simultaneously these inhibitors almost have no effect on strain growth.Streptomicete su gram pozitivne bakterije Äije je najvažnije staniÅ”te zemlja. Pri rastu na Ävrstim podlogama i u submerznim uslovima grade supstratni i vazduÅ”ni micelijum. MorfoloÅ”ka diferencijacija je praÄena produkcijom sekundarnih metabolita kao Å”to su antibiotici, a i razliÄitih hidrolitiÄkih enzima. Za mikroorganizme koji ih proizvode antibiotici predstavljaju kompetitivno oružje, agense za transport metala kao i efektore diferencijacije. ProteolitiÄki enzimi, takoÄe, imaju odreÄenu ulogu u razvojnom ciklusu aktinomiceta. Na primer, ukljuÄeni su u procese graÄenja vazduÅ”nog micelija i sporulaciju. Soj Streptomyces hygroscopicus CH-7 pri fermentaciji proizvodi antibiotike poliketidne strukture i smesu ekstracelularnih proteolitiÄkih enzima. U ovom radu je ispitivan uticaj antibiotika rifamicina i hloramfenikola, inhibitora transkripcije i translacije, na produkciju polienskog antibiotika heksaena H-85 i proteolitiÄkih enzima. Inhibited su dodavani pri inokulaciji (O h) i u razliÄitim vremenskim intervalima fermentacije (12-48 h). Dejstvom antibiotika inhibitora transkripcije i translacije utvrdjeno je da se enzim polien sintetaza transkribuje do 12. Äasa, a translacija teÄe do 48. Äasa fermentacije. Dodatak rifamicina pri zasejavanju drastiÄno snižava proteolitiÄku aktivnost, a u kasnijim fazama izaziva smanjenje (36-80%). Hloramfenikol smanjuje produkciju tripsinu sliÄne proteaze. ali nema dejstvo na aminopeptidaznu aktivnost. Rifamicin i hloramfenikol ne pokazuju znaÄajno inhibitorno dejstvo na rast soja
Study of tolerance of Pseudomonas aeruginosa san ai to mercury, cadmium and copper
Zagadenje zivotne okoline teskim metalima je ozbiljan problem savremenog sveta. MeÄu metalima koji su cesti zagadjivaci su: bakar, kadmijum i ziva. Cilj ovog rada je bilo ispitivanje osetljivosti soja Pseudomonas aeruginosa san ai na pomenute teske metale. Minimale inhibitome koncentracije kadmijuma i bakra od 2 i 4 mM, redom, pokazale su da je soj otporan na ova dva metala. MIK za zivu je bila najniza testirana koncentracija metala od 0,5 mM. Dobijene vrednosti MIK-aza kadmijum i bakar stavljaju soj P. aeruginosa san ai u grupu bakterija koje ispoljavaju visokutoleranciju prema ovim teskim metalima. Utvrdeno je daje ziva toksicna za soj. lspitivanja
mehanizama detoksikacije soja P. aeruginosa san ai u cilju potencijalne primene u procesima
bioremedijacije su u toku.The pollution of the environment with toxic heavy metals is spreading throughout the world along with industrial progress. Among the common heavy metals used and the more widespread
contaminants of the environment are mercury, cadmium and copper. The present research was
performed in order to screen tolerance of strain Pseudomonas aeruginosa san ai to these heavy
metals Study showed that P. aeruginosa san ai is tolerant to cadmium and copper with MIC's of 2
and4 mM, respectively. MIC for mercury was the lowest tested concentration of 0.5 roM. This
suggests that P. aeruginosa san ai has a large potential for bioremediation of environments
contaminated with cadmium and copper and that mercury is toxic for the strain. In order to
improve possible application of strain, P. aeruginosa san ai in processes of bioremediation the
study of mechanisams of metal uptake are underway
Efficient biodegradation of petroleum n-alkanes and polycyclic aromatic hydrocarbons by polyextremophilic Pseudomonas aeruginosa san ai with multidegradative capacity
Pseudomonas aeruginosa san ai, an alkaliphilic, metallotolerant bacterium, degraded individual selected
petroleum compounds, i.e., n-alkanes (n-hexadecane, n-nonadecane) and polycyclic aromatic
hydrocarbons (fluorene, phenanthrene, pyrene) with efficiency of 80%, 98%, 96%, 50% and 41%,
respectively, at initial concentrations of 20 mg L 1 and in seven days. P. aeruginosa san ai showed a high
biodegradative capacity on complex hydrocarbon mixtures, the aliphatic and aromatic fractions from
crude oil. The efficiency of P. aeruginosa san ai degradation of crude oil fractions in seven days reached
stage 3ā4 of the oil biodegradation scale, which ranges from 0 (no biodegradation) to 10 (maximum
biodegradation). Identified metabolites concomitant with genomic and enzymatic data indicated the
terminal oxidation pathway for the n-alkane degradation, and the salicylate and phthalate pathways for
fluorene biodegradation. Polyextremophilic P. aeruginosa san ai, as a biosurfactant producer with
multidegradative capacity for hydrocarbons, can be used in an improved strategy for environmental
bioremediation of hydrocarbon-contaminated sites, including extreme habitats characterized by low or
elevated temperatures, acidic or alkaline pH or high concentrations of heavy metals
Supplementary data for article: BiheloviÄ, F.; KaradžiÄ, I.; MatoviÄ, R.; SaiÄiÄ, R. Total Synthesis and Biological Evaluation of (-)-Atrop-Abyssomicin C. Organic and Biomolecular Chemistry 2013, 11 (33), 5413ā5424. https://doi.org/10.1039/c3ob40692j
Supplementary material for: [https://doi.org/10.1039/c3ob40692j]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1388
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