Effect of different final irrigation solutions on dentinal tubule penetration depth and percentage of root canal sealer

WOS: 000304691200026PubMed ID: 22595128Introduction: The purpose of this study was to evaluate the effects of different solutions used for final irrigation on sealer penetration into dentinal tubules. Methods: Thirty-two recently extracted human mandibular premolar teeth were treated with sodium hypochlorite (NaOCl) irrigation. The samples were divided into 4 groups according to the final irrigation solution used: (1) the EDTA group: 17% EDTA + 2.5% NaOCl, (2) the maleic acid (MA) group: 7% MA + 2.5% NaOCl, (3) the citric acid (CA) group: 10% CA + 2.5% NaOCl, and (4) the control group: 2.5% NaOCl. All teeth were obturated using the cold lateral condensation technique with gutta-percha and AH 26 sealer (Dentsply; DeTrey, Konstanz, Germany) labeled with fluorescent dye. The teeth were sectioned at distances of 2, 5, and 8 mm from the root apex. Total percentage and maximum depth of sealer penetration were measured using confocal laser scanning microscopy. Results: The Kruskal-Wallis analysis results showed that there was a significant difference in the percentage and maximum depth of sealer penetration among all groups in all sections (P<.05). The coronal sections in each group showed a significantly higher percentage and maximum depth of sealer penetration than did the apical and middle sections (P<.05). Conclusions: Final irrigation with EDTA, MA, and CA after the use of NaOCl affected sealer penetration. However, there was no significant difference between these experimental groups (EDTA, MA, and CA) in all sections

Effect of Different Final Irrigation Solutions on Dentinal Tubule Penetration Depth and Percentage of Root Canal Sealer

Introduction: The purpose of this study was to evaluate the effects of different solutions used for final irrigation on sealer penetration into dentinal tubules. Methods: Thirty-two recently extracted human mandibular premolar teeth were treated with sodium hypochlorite (NaOCl) irrigation. The samples were divided into 4 groups according to the final irrigation solution used: (1) the EDTA group: 17% EDTA + 2.5% NaOCl, (2) the maleic acid (MA) group: 7% MA + 2.5% NaOCl, (3) the citric acid (CA) group: 10% CA + 2.5% NaOCl, and (4) the control group: 2.5% NaOCl. All teeth were obturated using the cold lateral condensation technique with gutta-percha and AH 26 sealer (Dentsply; DeTrey, Konstanz, Germany) labeled with fluorescent dye. The teeth were sectioned at distances of 2, 5, and 8 mm from the root apex. Total percentage and maximum depth of sealer penetration were measured using confocal laser scanning microscopy. Results: The Kruskal-Wallis analysis results showed that there was a significant difference in the percentage and maximum depth of sealer penetration among all groups in all sections (P<.05). The coronal sections in each group showed a significantly higher percentage and maximum depth of sealer penetration than did the apical and middle sections (P<.05). Conclusions: Final irrigation with EDTA, MA, and CA after the use of NaOCl affected sealer penetration. However, there was no significant difference between these experimental groups (EDTA, MA, and CA) in all sections. (J Endod 2012;38:860-863

Pulp Diagnostic Tests

Pulp diagnostic tests evaluate the neuropsychological status and blood flow of the pulp. These tests can be used in conjunction with other clinical tests, such as periodontal probing, percussion, palpation, mobility, and anesthesia, during diagnostic tests. In clinical practice, it is important to determine the pulp condition in deciding the correct treatment to be applied. The aim of this review is to provide information about the pulp diagnostic tests that are used in dental practice and to generalize the correct use of these tests

Fracture resistance of endodontically treated molars restored with polyethylene fiber and different posts

The purpose of this study was to compare the fracture strength and mode of teeth restored with fiber/titanium post, polyethylene fiber, and adhesive composite. The mesial, distal, and palatal walls of human maxillary molar teeth were removed, so that only the buccal wall remained. Group 1, with caries-free maxillary molars, was used as a positive control group and the remaining groups were restored as follows: group 2, with only adhesive composite; group 3, with polyethylene fiber and adhesive composite; group 4, with fiber post and adhesive composite; group 5, with fiber post, polyethylene fiber, and adhesive composite; group 6, with titanium post and adhesive composite; and group 7, with titanium post, polyethylene fiber, and adhesive composite. A universal testing machine was used for fracture tests. Compressive loads were applied at an angle of 90 degrees on the occlusal surface of the specimens at crosshead speed of 1 mm/min until fracture occurred. Kruskal-Wallis and Mann-Whitney U tests were adopted for statistical analysis. The study shows that, based on the fracture strength, the group of teeth that were restored with glass fiber post, polyethylene fiber, and adhesive composite has the most significant improvement over all the other teeth groups. Based on the fracture mode, the teeth groups restored with only glass fiber post, adhesive composite, polyethylene fiber, and adhesive composite have relatively more restorable fractures observed

Correlation between sealer penetration into dentinal tubules and bond strength of two new calcium silicate-based and an epoxy resin-based, endodontic sealer

The relationship between sealer penetration into dentinal tubules and the bond strength of two new calcium silicate-based and epoxy resin-based endodontic sealers was evaluated. Thirty recently extracted human maxillary incisors were instrumented and divided into three groups according to the sealer used: (1) AH Plus; (2) iRoot SP; and (3) MTA Fillapex. Sealer penetration into dentinal tubules was measured using CLSM. Then, a universal testing machine was used to compare the push-out bond strengths of the sealers to the root canal dentin. AH Plus and MTA Fillapex exhibited significantly higher sealer penetration than iRoot SP did (p > .05). The push-out bond strengths of AH Plus and iRoot SP were higher than that of MTA Fillapex. We concluded that greater penetration of the sealer into the dentinal tubules was not associated with higher bond strength among the three sealers tested

Effect of root canal treatment on marginal bone levels of chronic periodontitis patients

Amaç: Periodontitis esas olarak mikrobiyal dental plağın neden olduğu lokal ve sistemik faktörlerin de rol aldığı, alveoler kemikte yıkıma neden olan kronik bir enflamasyondur. Restorasyonlar ve kök kanal tedavisi periodontal yıkıma neden olabilecek lokal risk faktörleri olarak görülmektedir. Bu çalışmanın amacı periodontitis hastalarında kök kanal tedavisinin marjinal kemik seviyesine etkisinin incelenmesidir. Gereç ve Yöntem: Kronik periodontitis teşhisi konulmuş hastalara ait ortopantomograf filmler incelenerek kök kanal tedavili büyük azı dişler simetriğinde kök kanal tedavisi yapılmamış, aynı koronal restorasyona sahip dişler ile kıyaslanarak mesiyal ve distal taraflardaki marjinal kemik seviyeleri ölçülmüştür. Bulgular: Kök kanal tedavili dişler ile kök kanal tedavisi uygulanmayan restorasyonlu dişlerin mesiyal ve distal kemik seviyesi ortalamaları arasında istatistiksel olarak anlamlı bir farklılık bulunmamaktadır (p>0.05). Sonuç: Kronik periodontitisli hastalarda ideal kök kanal tedavisinin marjinal kemik seviyesi üzerinde direkt etkisi olmadığı gösterilmiştirPurpose: Periodontitis is a chronic inflammation mainly caused by dental plaque; local and systemic factors may also be involved in chronic inflammation that causes destruction of the alveolar bone. Restorations and root canal treatment is seen as a local risk factors that may cause destruction of the periodontal bone. The aim of this study was to investigate radiographically the effect of root canal treatment on periodontal bone level in periodontitis patients. Material and Methods: Bone level of endodontically treated tooth and its contra-lateral tooth without root canal treatment at the mesial and distal sides of these teeth was scored. Results: No difference was found statistically between the periodontol bone level of endodontically treated tooth and its contralateral tooth without root canal treatment (p>0.05). Conclusion: It was determined that adequate root canal therapy in patients with chronic periodontitis did not have a direct impact on the marginal bone level

Inhibition of cell survival, viability and proliferation by dentin adhesives after direct and indirect exposure in vitro

WOS: 000311362300016PubMed ID: 22222515Objectives The influence of dentin adhesive systems (Scotchbond Multi-Purpose, XP Bond, Xeno V, Clearfil Protect Bond, AdheSE) on cell survival, viability and proliferation was characterized after direct and indirect exposure using different cell culture techniques. Materials and methods The primers and cured bonding parts were directly exposed to cells using cell culture inserts, and complete materials were analyzed in a dentin barrier test. Cell responses were examined in 3T3 mouse fibroblasts after 24- and 72-h exposure periods by the estimation of total cell numbers (survival), apoptosis (viability) and cell proliferation. Results Cell numbers were effectively reduced by the primers of AdheSE, Protect Bond, and Scotchbond Multi-Purpose as well as XP bond after direct exposure in a cell culture insert test device. Likewise, Scotchbond Multi-Purpose primer induced a rate of apoptosis (93.9%) even higher than detected with Protect Bond primer (91.6%). Cell proliferation was entirely inhibited by primers and by Xp Bond as well. The Scotchbond Multi-Purpose was most cytotoxic in a dentin barrier test device after a 24-h indirect exposure. It also increased the percentage of cells in apoptosis to 15.4% compared to untreated controls. Conclusion Unpolymerized primers of dentin adhesives were more cytotoxic than polymerized bonding counterparts. Moreover, total etch dentin adhesives were more cytotoxic than self-etch adhesives. Clinical relevance When dentin adhesives are used in deep cavities without a protective dentin barrier the leachable hydrophobic and hydrophilic component of dentin adhesive systems can penetrate to the pulp and may induce cytotoxic responses in pulp tissues.Scientific Research Projects Coordination Unit of Istanbul University [3230]The current study is based on a thesis submitted to the graduate faculty, University of Istanbul, in partial fulfillment of the requirements for the degree of Doctor of Philosophy and was supported by Scientific Research Projects Coordination Unit of Istanbul University. Project number 3230