Method of studying depth distribution of therapeutic and diagnostic substances inside spherical amorphous nanoparticles

A method for investigating the distribution of therapeutic and diagnostic substances inside nanoparticles was developed for spherical amorphous nanoparticles (SANPs). SANPs were generated from the lupan triterpenoids of birch bark (LTs). This method was based on the sequence of short exposures of SANPs in a special solvent. The thin surface layers of the main material and the material of substances incorporated into the SANPs were dissolved. The distribution of three different substances in the SANPs were analyzed by determining the optical density of the solutions containing material extracted from SANPs. It was shown that the distribution of therapeutic and diagnostic substances in SANPs depended on their solubility in the solution saturated with the main SANPs material and their mass fraction. © 2010 Pleiades Publishing, Ltd

Method of studying depth distribution of therapeutic and diagnostic substances inside spherical amorphous nanoparticles

A method for investigating the distribution of therapeutic and diagnostic substances inside nanoparticles was developed for spherical amorphous nanoparticles (SANPs). SANPs were generated from the lupan triterpenoids of birch bark (LTs). This method was based on the sequence of short exposures of SANPs in a special solvent. The thin surface layers of the main material and the material of substances incorporated into the SANPs were dissolved. The distribution of three different substances in the SANPs were analyzed by determining the optical density of the solutions containing material extracted from SANPs. It was shown that the distribution of therapeutic and diagnostic substances in SANPs depended on their solubility in the solution saturated with the main SANPs material and their mass fraction. © 2010 Pleiades Publishing, Ltd

Lipoilcarnosine: Synthesis, study of physico-chemical and antioxidant properties, biological activity

Synthesis of lipoilcarnosine (LipC) - a conjugated molecule based on two natural antioxidants, carnosine and a-lipoic acid, is described. Its physico-chemical, antioxidant properties and biological activity are characterized. According to reversed-phase HPLC with a UV detector, purity of the final product was 89.3%. The individuality of the obtained sodium salt of LipC was confirmed by tandem HPLC-mass spectrometry. High resistance of LipC to hydrolysis with serum carnosinase was demonstrated. The antioxidant activity of LipC measured by reaction with the formation of thiobarbituric acid reacting substances and kinetic parameters of iron-induced chemiluminescence was higher than that of carnosine and lipoic acid. LipC did not affect viability of SH-SY5Y human neuroblastoma culture cells, differentiated towards the dopaminergic type, at concentrations not exceeding 5 mM. At the concentration range of 0.1-0.25 mM LipC protected neuronal cells against 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity. © 2018 Russian Academy of Medical Sciences. All rights reserved

Lipoylcarnosine: Synthesis, Study of Physico-Chemical and Antioxidant Properties, Biological Activity

Abstract—: Synthesis of lipoylcarnosine (LipС), a conjugated molecule based on two natural antioxidants, carnosine and α-lipoic acid, is described and its physico-chemical, antioxidant properties and biological activity are characterized. According to reversed-phase HPLC with a UV detector, purity of the final product was 89.3%. The individuality of the obtained sodium salt of LipС was confirmed by tandem HPLC-mass spectrometry. LipC demonstrated high resistance to hydrolysis with serum carnosinase. The antioxidant activity of LipC evaluated by the reaction with the formation of thiobarbituric acid reacting substances and kinetic parameters of iron-induced chemiluminescence was higher than that of carnosine and lipoic acid. LipC did not affect viability of SH-SY5Y human neuroblastoma cells, differentiated to the dopaminergic phenotype, at concentrations not exceeding 5 mM. In the concentration range of 0.1–0.25 mM LipC protected neuronal cells against 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity. © 2018, Pleiades Publishing, Ltd

Lipoilcarnosine: Synthesis, study of physico-chemical and antioxidant properties, biological activity

Synthesis of lipoilcarnosine (LipC) - a conjugated molecule based on two natural antioxidants, carnosine and a-lipoic acid, is described. Its physico-chemical, antioxidant properties and biological activity are characterized. According to reversed-phase HPLC with a UV detector, purity of the final product was 89.3%. The individuality of the obtained sodium salt of LipC was confirmed by tandem HPLC-mass spectrometry. High resistance of LipC to hydrolysis with serum carnosinase was demonstrated. The antioxidant activity of LipC measured by reaction with the formation of thiobarbituric acid reacting substances and kinetic parameters of iron-induced chemiluminescence was higher than that of carnosine and lipoic acid. LipC did not affect viability of SH-SY5Y human neuroblastoma culture cells, differentiated towards the dopaminergic type, at concentrations not exceeding 5 mM. At the concentration range of 0.1-0.25 mM LipC protected neuronal cells against 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity. © 2018 Russian Academy of Medical Sciences. All rights reserved