5 research outputs found
Zatorska goose - a subject of parasitological research
The aim of the study was to determine the level of gastrointestinal parasites in a native breed of geese –
Zatorska goose – based on coproscopic testing. Faecal samples were collected from 90 young geese in three age groups
(5, 7 and 9 weeks old) in 2014. The geese were kept indoors on deep litter and pastured from spring to autumn. The area
of the pastures around the buildings where the geese grazed was about 1 hectare, divided into quarters for different age
groups. Before grazing, the birds were dewormed with fenbendazole (Fenbenat powder 4%, Naturan). As additional
treatment for coccidiosis, coccidiostats were added to the feed. The study was conducted using the McMaster
quantitative method with centrifugation (flotation liquid: NaCl and glucose). The birds were shown to be infected with
coccidia and nematodes. The prevalence of Eimeria sp. infection (mean 40%) and the number of oocysts per gram of
faeces (reaching 5,300 OPG) were highest in the youngest age group of geese. The level of Amidostomum anseris
infection was similar in the three age groups, with prevalence from 40% to 50% (nematode egg output ranged from 50
to 350 eggs per gram of faeces, EPG). Capillaria anatis was observed only in 5- and 7-week-old geese
Applicability of anatid and galliform microsatellite markers to the genetic diversity studies of domestic geese (<it>Anser anser domesticus</it>) through the genotyping of the endangered zatorska breed
Abstract Background The lack of a sufficient number of molecular markers seriously limits the cognition of genetic relationships within and between populations of many species. Likewise, the genetic diversity of domestic goose (Anser anser domesticus), with a great number of breeds throughout the world, remains poorly understood at the molecular level. Findings Thirty-five goose, seventeen duck and eight chicken microsatellite primer pairs were screened for their utility in the cross-species amplification on DNA from 96 individuals of Zatorska breed of domestic geese. Twenty-seven of 42 amplifying primer pairs revealed length-polymorphic products, but three of them were difficult to score. Fifteen primer pairs amplifying the same length product across all individuals. One polymorphic microsatellite locus was assigned by genotyping of known sex individuals to the Z-chromosome. Conclusions We present a set of 24 polymorphic microsatellite markers useful for population genetic studies of the domestic goose. Another 15 markers were classified as monomorphic, but they might also be suitable for the assessment of genetic diversity in geese.</p
Preliminary Studies on Genetic Diversity of Selected Polish Local Chicken Varieties
The study was aimed at evaluation of the applicability of selected microsatellite markers in examining genetic diversity in five Polish local chicken varieties (two groups of native crested chickens, crestless native chickens from Subcarpathian region and two groups of native miniature chickens) and reveal their genetic relationships to five purebreds (Leghorn, Rhode Island Red, Sussex, Greenleg Partridge, Minorca). About 22.8% (overall FST = 0.228) of the genetic variation was explained by differences between breeds and varieties. The DA genetic distances between five Polish native varieties and five purebreds varied between 0.154 and 0.440. The results obtained in this study indicate the great value of the collection and suggest the need for formal protection of these local Polish chicken varieties