Dispersion of T lymphoblastoid leukemia cells on a cell microarray chip and confinement in the microchambers.

<p>(A, B) Photographic light microscopic images of T lymphoblastoid leukemia cells incubated on a cell microarray chip before (A) and after (B) washing of the chip surface. (C–F) Photos of microchamber appearance after washing when T lymphoblastoid leukemia suspensions of 2.5×10⁶ (C), 5.0×10⁶ (D), 7.5×10⁶(E) or 1.0×10⁷ (F) cells/ml were applied to the microarray chip. Concentrations of 7.5×10⁶ cells/ml of T lymphoblastoid leukemia and above afforded tight confinement and formation of a monolayer in the microchambers. (Bar: 20 µm).</p

Dispersion of leukocytes isolated from whole blood on a cell microarray chip and confinement in the microchambers.

<p>(A, B) Photographic light microscopic images of leukocytes on a cell microarray chip before (A) and after (B) washing of the chip surface. (C) The leukocytes showed tight confinement and had formed a monolayer in the microchamber. (Bar: 20 µm).</p

Scanned images of double-stained cells.

<p>(A–L) Cultured T lymphoblastoid leukemia (A–F) and leukocytes isolated from whole blood (F–L) were stained with PE-labeled anti-cytokeratin monoclonal antibody (A, G) and APC-labeled anti-EpCAM monoclonal antibody (C, I). (E, K) Merged images identify doubly-positive carcinoma cells in each panel. Magnified views of the boxed regions (B, D, F, H, J, L). Scatter-plot analysis of 3 cluster areas representing 561 microchambers in a cell microarray chip (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032370#pone-0032370-g009" target="_blank">Fig. 9M</a>).</p

Dispersion of carcinoma cells on a cell microarray chip and confinement in the microchambers.

<p>(A, B) Photographic light microscopic images of carcinoma cells on a cell microarray chip before (A) and after (B) washing of the chip surface. (C) Carcinoma cells showed tight confinement and had formed a monolayer in the microchamber when a concentration of 7.5×10⁶ cells/ml was used. (Bar: 20 µm).</p

Fluorescence detection of T lymphoblastoid leukemia and carcinoma cells in the microchambers.

<p>(A,D) Scanning images of T lymphoblastoid leukemia (A) and carcinoma cells (D) stained with APC-labeled anti-CD 45 monoclonal antibodies in 128 microchambers. (B, E) Magnified views of the boxed regions in “A” and “D,” respectively. (C, F) Light microscopic images of microchambers in “B” and “E,” respectively, showing tight confinement of the cells as a monolayer. (G, J) Scanning images of leukocytes (G) and carcinoma cells (J) stained with PE-labeled anti-cytokeratin monoclonal antibodies in 128 microchambers. (H, K) Magnified views of the boxed regions in “G” and “J.” (I, L) Light microscopic images of microchambers in “H,” “K,” respectively, showing cells tightly confined as a monolayer. (M, P) Scanning images of leukocytes (M) and carcinoma cells (P) stained with DiD in 128 microchambers. (N, Q) Magnified views of the boxed regions in “M” and “P,” respectively. (O, R) Light microscopic views of microchambers in “N” and “Q,” respectively, showing tight confinement of cells as a monolayer. (Bar: 20 µm). Color scale at the right represents the intensity of fluorescent emission.</p

Detection of carcinoma cells among cultured T lymphoblastoid leukemia on a cell microarray chip.

<p>(A–I) Scanned images of leukocytes/carcinoma cells on a cell microarray chip obtained with the microarray scanner. (A) Negative control (no carcinoma cells). (B, D, G) Carcinoma cells (0.01, 0.001, and 0.0001%) were scanned in 3, 9, and 64 clusters, respectively, on the cell microarray chip. (C, E, F, H, I) Magnified views of the boxed regions. Color scale represents the intensity of fluorescent emission.</p

Detection of carcinoma cells among leukocytes/carcinoma cells isolated from whole blood and introduced onto a cell microarray chip.

<p>(A) Scanned images of cells on a cell microarray chip, obtained with the microarray scanner. The cells were immunostained with PE-labeled anti-cytokeratin monoclonal antibody. (B) Magnified view of the boxed region. Color scale represents the intensity of fluorescent emission.</p

Analysis of spiked carcinoma cells in human whole blood on a cell microarray chip.

<p>*Samples no. 1 to 4 were spiked with 50 carcinoma cells; and 5 to 7, were spiked with 500 carcinoma cells,</p><p>**Ten microchambers were randomly selected on a cell microarray chip, and the number of carcinoma cells in each of the chambers was counted.</p

Schematic process for detection of PICP on a microchip with deposition and fixation of 1st antibody by piezoelectric ink printing.

<p>After fixation of antibody on the surface of the microchannel and sealing with a cover film, blocking solution was infused into each microchannel from the reservoir (wells 1, 3, 5, and 7) by use of a pipetman.</p

Reproducibility of luminescence intensity of PICP in 1 channel.

<p>Reproducibility of luminescence intensity of PICP in 1 channel.</p