Nano-lactoferrin in diagnostic, imaging and targeted delivery for cancer and infectious diseases

Lactoferrin (Lf) is a natural occurring iron binding protein present in many mammalian excretions and involved in various physiological processes. Lf is used in the transport of iron along with other molecules and ions from the digestive system. However its the modulatory functions exhibited by Lf in connection to immune response, disease regression and diagnosis that has made this protein an attractive therapeutic against chronic diseases. Further, the exciting potentials of employing nanotechnology in advancing drug delivery systems, active disease targeting and prognosis have also shown some encouraging outcomes. This review focuses on the role of Lf in diagnosing infection, cancer, neurological and inflammatory diseases and the recent nanotechnology based strategies

Diagnostic Imaging of Canine Hepatobiliary Affections: A Review

Hepatic disease is often treatable and has a predictable prognosis when a definitive diagnosis is made. The aim of clinicopathological evaluation of hepatobiliary affections is to identify and characterize hepatic damage and dysfunction, identify possible primary causes of secondary liver disease, differentiate causes of icterus, evaluate potential anaesthetic risks, assess prognosis and response to xenobiotics, and monitor response to therapy. This paper describes the different diagnostic methods and imaging techniques employed in diagnosis of hepatobiliary affections in dogs. Besides reviewing the significant clinical manifestations and imaging structural abnormalities in diagnostic approach to different hepatic affections, it also depicts radiographic, ultrasonographic, and wherever applicable, the laparoscopic characterization of different hepatic affections and target lesions encountered in clinical cases presented in the Teaching Veterinary Clinical Complex, COVAS, Palampur in the year 2007-2008

Flavour structure of low-energy hadron pair photoproduction

We consider the process $\gamma\gamma\to H_1\bar H_2$ where $H_1$ and $H_2$ are either mesons or baryons. The experimental findings for such quantities as the $p\bar p$ and $K_SK_S$ differential cross sections, in the energy range currently probed, are found often to be in disparity with the scaling behaviour expected from hard constituent scattering. We discuss the long-distance pole--resonance contribution in understanding the origin of these phenomena, as well as the amplitude relations governing the short-distance contribution which we model as a scaling contribution. When considering the latter, we argue that the difference found for the $K_SK_S$ and the $K^+K^-$ integrated cross sections can be attributed to the s-channel isovector component. This corresponds to the $\rho\omega\to a$ subprocess in the VMD (vector-meson-dominance) language. The ratio of the two cross sections is enhanced by the suppression of the $\phi$ component, and is hence constrained. We give similar constraints to a number of other hadron pair production channels. After writing down the scaling and pole--resonance contributions accordingly, the direct summation of the two contributions is found to reproduce some salient features of the $p\bar p$ and $K^+K^-$ data.Comment: 12 pages, 9 figures, revised version to be published in EPJ

Farming Systems Research in India: A Historical Perspective

This review gives a historical perspective of the development of Farming Systems Research (FSR) in India over the past 60 years, as India changed from a traditional, subsistence oriented agriculture to one based on science and technology. The first period relates to the years 1930–50, when the Government of India created the Indian Council of Agricultural Research (ICAR) and initiated research on dryland agriculture The second period relates to the development of coordinated agronomic trials and simple fertilizer experiments on farmers' fields during the years 1950–65. This development was a forerunner of networks of coordinated applied agronomic research relevant to farmers' cropping systems and adaptive research with farmers' participation. The development of a soil conservation research network in the same period strengthened research on the natural resource base countrywide. The third period started with the reorganization of ICAR, the establishment of State Agricultural Universities and the introduction of coordinated research programmes on high-yielding varieties (HYVs). At the same time the coordinated dryland agricultural research programmes, and subsequently the Central Research Institute for Dryland Agriculture (CRIDA), were established, leading to the development of a national system of FSR for rainfed agriculture The fourth period of FSR started with the establishment in 1972 of ICRISAT, an international agricultural research centre that accepted FSR as its mandate and developed the concept of integrating the management technologies for various components of climate, soil, water and crops with a farmers' perspective. ICRISAT's work was complementary to that of CRIDA and helped to bring about conceptual changes in Indian FSR through research aimed at understanding principles and processes of semi-arid tropical (SAT) farming systems. Following this, mechanisms for strengthening on-farm research were emphasized so as to provide stronger linkages between researchers, extension workers and farmers Today, FSR with a farmers' perspective occupies pride of place in India's agricultural research agenda. Yet it is a long way from bridging the gap between the generation of technology and its adoption by farmers, who have shown a preference for its components rather than for the full package of technolog

Progress on Azadirachta indica based biopesticides in replacing synthetic toxic pesticides

Over the years, extensive use of commercially available synthetic pesticides against phytophagous insects has led to their bioaccumulation in the environment causing increased resistance and reduction in soil biodiversity. Further, 90% of the applied pesticides enter the various environmental resources as a result of run-off, exposing the farmers as well as consumers of the agricultural produce to severe health issues. Therefore, growing attention has been given toward the development of alternate environmentally friendly pesticides/insecticides that would aid an efficient pest management system and also prevent chronic exposures leading to diseases. One such strategy is, the use of neem plant\u27s (Binomial name: Azadirachta indica) active ingredients which exhibit agro-medicinal properties conferring insecticidal as well as immunomodulatory and anti-cancer properties. The most prominent constituent of neem is azadirachtin, which has been established as a pivotal insecticidal ingredient. It acts as an antifeedant, repellent, and repugnant agent and induces sterility in insects by preventing oviposition and interrupting sperm production in males. This review discusses, key neem pesticidal components, their active functional ingredients along with recent strategies on employing nanocarriers, to provide controlled release of the active ingredients and to improve their stability and sustainability

Synthesis of ethyl oleate employing synthetic hydrogel-immobilized lipase of Bacillus coagulans MTCC-6375

Ten polymeric hydrogels were chemically synthesized by varying the concentrations of copolymer (DMA) and cross-linker (MBAm) molecules. An alkaline lipase of Bacillus coagulans MTCC-6375 was immobilized onto a poly (MAc-co-DMA-cl-MBAm)-hydrogel support at pH 8.5 and temperature 55&ordm;C in 16 h. The bound lipase possessed 7.6 U.g⁻&sup1; (matrix) lipase activity with a specific activity of 18 U.mg⁻&sup1; protein. Hydrogel bound-lipase catalyzed esterification of oleic acid and ethanol to synthesize ethyl oleate in n-nonane. Various kinetic parameters were optimized to produce ethyl oleate using immobilized lipase. The optimal parameters were bound enzyme/substrate (E/S) ratio 0.62 mg/mM, ethanol/oleic acid 100 mM:75 mM or 100 mM:100 mM, incubation time 18 h and reaction temperature 55&ordm;C that resulted in approximately 53% conversion of reactants into ethyl oleate in n-nonane. However, addition of a molecular sieve to the reaction mixture promoted the conversion to 58% in 18 h in n-nonane, which was equivalent to 55 mM of ethyl oleate produced.<br /

Cissus quadrangularis inhibits IL-1β induced inflammatory responses on chondrocytes and alleviates bone deterioration in osteotomized rats via p38 MAPK signaling

INTRODUCTION: Inflammatory mediators are key players in the pathogenesis of osteoarthritis (OA) and bone destruction. Conventional drugs suppress symptomatic activity and have no therapeutic influence on disease. Cissus quadrangularis and Withania somnifera are widely used for the treatment of bone fractures and wounds; however, the cellular and molecular mechanisms regulated by these herbals are still unclear. METHODS: We established an in vitro OA culture model by exposing human chondrocytes to proinflammatory cytokine and interleukin (IL)-1&beta; for 36 hours prior to treatment with the herbals: C. quadrangularis, W. somnifera, and the combination of the two herbals. Cell viability, toxicity, and gene expression of OA modifying agents were examined. In addition, expression of survivin, which is crucial for cell growth, was analyzed. In vivo work on osteotomized rats studied the bone and cartilage regenerative effects of C. quadrangularis, W. somnifera, and the combination therapy. RESULTS: Exposure of chondrocytes to IL-1&beta; induced significant toxicity and cell death. However, herbal treatment alleviated IL-1&beta; induced cell toxicity and upregulated cell growth and proliferation. C. quadrangularis inhibited gene expression of cytokines and matrix metalloproteinases, known to aggravate cartilage and bone destruction, and augmented expression of survivin by inhibiting p38 MAPK. Interestingly, osteotomized rats treated with C. quadrangularis drastically enhanced alkaline phosphatase and cartilage tissue formation as compared to untreated, W. somnifera only, or the combination of both herbals. CONCLUSION: Our findings demonstrate for the first time the signaling mechanisms regulated by C. quadrangularis and W. somnifera in OA and osteogenesis. We suggest that the chondroprotective effects and regenerative ability of these herbals are via the upregulation of survivin that exerts inhibitory effects on the p38 MAPK signaling pathway. These findings thus validate C. quadrangularis as a potential therapeutic for rheumatic disorders

Genetic diversity analysis of chrysanthemum (Chrysanthemum grandiflorum) cultivars using RAPD markers

Identification and characterization of new cultivars is essential to meet DUS testing, address IPR issues and their utilization and conservation. Traditionally, morphological markers were used for germplasm characterization as being simple and irreplaceable. A total of 57 RAPD primers were screened and out of these 22 primers which gave sufficient amplification were selected for the study. Out of 207 bands generated, 175 were polymorphic with a polymorphism percentage of 84.54%. The polymorphism percentage ranged between 60% (OPA04) to 100% (OPA17, OPB-06, OPX14, OPX-19). The primer OPA08 generated least numbers of bands (4) and OPA06 generated maximum number of bands (17). The Rp values ranged between 0.82 to 8.18 for RAPD primers OPY-4 and OPX16, respectively with a mean value of 3.94 and the correlation between Rp and number of cultivars identified by each primer was fairly high (0.96). The primers OPX16, OPY6 and OPA04 distinguished higher number of cultivars of 49, 46 and 37 respectively. The UPGMA dendrogram indicated that the cultivars Maghi Orange and Maghi Yellow were sharing the maximum similarity (89%) and were close to Maghi White at a similarity level of 86%. The other cultivars sharing higher similarity levels were Flirt with Yellow Gold, Pink Cloud with Korean Small and Sadbhawna with Jubilee. RAPD proved to be useful for the characterization of the genotypes for their efficient utilization, management and IPR protection

Pseudoscalar-pole contributions to the muon g−2g-2 at the physical point

Pseudoscalar-pole diagrams are an important component of estimates of the hadronic light-by-light (HLbL) contribution to the muon g−2. We report on our computation of the transition form factors P→γ∗γ∗ for the neutral pseudoscalar mesons P=π0 and η . The calculation is performed using twisted-mass lattice QCD with physical quark masses. On the lattice, we have access to a broad range of (space-like) photon four-momenta and therefore produce form factor data complementary to the experimentally accessible single-virtual direction, which directly leads to an estimate of the pion- and η -pole components of the muon g−2 . For the pion, our result for the g−2 contribution in the continuum is comparable with previous lattice and data-driven determinations, with combined relative uncertainties below 10% . For the η meson, we report on a preliminary determination from a single lattice spacing