Nano microbe zapper (NMZ): a novel eco-safe nanoparticle mediated antimicrobial killer

This presentation introduced a new antimicrobial spray

Nanomedicine approach for sustained release delivery of Avastin : treatment for PXE and AMD [abstract]

Gold nanoparticles possess unique properties including preferential binding to leaky blood vessels, ability to bind to a variety of ligands, with no evidence of cellular toxicity, making them an excellent platform for targeted sustained release of drugs. Avastin (Bevacizumab) is a humanized monoclonal antibody specifically targeting vascular endothelial growth factor (VEGF) that has found widespread use in inhibiting intraocular neovascularization manifested in macular degeneration and proliferative diabetic retinopathy. The conjugation of gold nanoparticles (AuNP) with Avastin (Av) yields AvAuNP nanoconjugates. Avastin conjugated gold nanoparticles (AvAuNP) can be used as therapeutic agents in the treatment of ophthalmic neovascular disorders, such as macular degeneration, PXE and proliferative diabetic retinopathy. AvAuNP nanoconjugate is a potential clinical therapeutic agent and has demonstrated excellent ability to deliver Avastin for sustained release of therapeutic dose within the eye. The design and development of AvAuNP conjugate would help in the initiation and completion of preclinical evaluations aimed at determining the ability to achieve long-term suppression of intraocular neovascularization in large animals. INVENTOR(S): Ravi Shukla; Kavita K. Katti; Raghuraman Kannan; Dean Hainsworth and Kattesh V. Katti CONTACT INFO: Paul Hippenmeyer, Ph.D., M.B.A.; [email protected]; (573)-882-047

A Novel Method to Monitor Sequential Displacement of Capped Ligands in Gold Nanoparticles [abstract]

Nanoscience Poster SessionNanochemistry of ligand displacement reactions has attracted much attention in recent years for the development of myriad of new gold nanomaterials. Gold nanoparticles have shown applications ranging from tumor imaging agent in nanomedicine to single electron devices in information technology. New gold materials are synthesized by exchange of neutral or anionic ligands with thiolated molecules. Completion of ligand substitution reactions in gold nanoparticles are monitored by using UV-Vis spectrometry. However, there are no methods available to monitor the sequence of the ligand substitution reactions. Monitoring and predicting the sequence of ligand substitutions would provide a convenient handle for the design and development of hybrid nanomaterials containing two or more ligands. In this context, we have developed a novel technique utilizing disc centrifuge systems to monitor the sequential displacement of ligands in various gold nanoconstructs. In our studies, we have used gold nanoparticles stabilized with both anionic and neutral ligands. Gold nanoparticles of various different substitutions have been identified and characterized by disc centrifuge systems. Details of substitution reactions and mechanism on monitoring the sequential displacement using strong ligands will be presented

Production and optimization of 198/199 gold nanoparticles for potential use in cancer therapy [abstract]

Abstract only availableRadiopharmaceuticals are used to diagnose and treat a number of diseases such as bone cancer and non-Hodgkin's lymphoma. A radiopharmaceutical typically consists of a targeting molecule that selectively targets certain tumors. The targeting molecule is labeled with a radioactive atom(s) that delivers a dose of radiation to the tumor. The radioactive properties of Au-198 ([beta]- = 0.96 MeV; [gamma] = 411 KeV) and Au-199 ([beta]- = 0.45 MeV; [gamma] = 158 KeV) with their beta (therapeutic) and gamma (imaging) emission make them valuable candidates for both therapeutic and imaging applications. Gold nanoparticles have several properties that make them particularly interesting for use in radiopharmaceuticals. They are stable in vivo, have multiple atoms per particle and are small enough in size to deliver a radioactive dose directly to cancer cells. The purpose of this study was to gain a better understanding of the binding properties of the nanoparticles with reducing and stabilizing agents. This knowledge will aid in future attempts to label the particles with various antibodies and peptides for tumor targeted delivery of the drug. Next, investigate the relationship between particle size and the amount of reducing agent used was studied with varying amounts and types of carbohydrate stabilizers. Our goal is to establish a library of nanoparticles with varying sizes that can be conjugated with different biomolecules that are selective for receptors over expressed by the diseased tissue. In future studies we also plan to pursue an indirect method of preparing radioactive Au-199 nanoparticles at carrier free levels from beta decay of Pt-199.Life Sciences Undergraduate Research Opportunity Progra

A Comparative Study between Antibody and Peptide Conjugated Gold Nanoparticles for In Vivo Targeting of EGFR in Pancreatic Cancer Bearing Mice Models [abstract]

Nanoscience Poster SessionPancreatic cancer is the fourth leading cause of cancer related deaths in the United States due to its severe aggressiveness and lethal malignancy. Epidermal Growth Factor Receptor (EGFR) is over expressed in more than 95% of human pancreatic cancer patients. A number of peptides and monoclonal antibodies have been developed to target the EGFR in pancreatic cancer. Our research has focused on developing EGFR targeting biomolecule conjugated gold nanoparticles for the diagnosis and staging of various cancers. In this study, we synthesized a series of Antibody EGFR and EGFR-peptide conjugated AuNPs. We investigated the in vivo EGFR targeting characteristics of these conjugates in pancreatic tumor bearing SCID mice models. Our investigation establishes that the peptide conjugated AuNPs have high in vivo mobility and targets pancreatic tumor effectively. We have also established that EGFR-peptide -AuNP conjugates act as better X-ray contrast agents for early detection of pancreatic cancer in mice models. The details of this comparative study will be presented in this poster

Relative study between anti-EGFR and GE-11 peptide conjugated gold nanoparticles for in vivo targeting in pancreatic cancer [abstract]

Pancreatic cancer is the fourth leading cause of cancer related deaths in the United States due to its severe aggressiveness and lethal malignancy. Epidermal Growth Factor Receptor (EGFR) is over expressed in more than 95% of human pancreatic cancer patients. A number of peptides and monoclonal antibodies have been developed to target the EGFR in pancreatic cancer. Our research has focused on developing EGFR targeting biomolecule conjugated gold nanoparticles for the diagnosis and staging of various cancers. In this study, we have synthesized a series of Antibody EGFR and EGFR-peptide (GE-11) conjugated AuNPs. We investigated the in vivo EGFR targeting characteristics of these conjugates in pancreatic tumor bearing SCID mice models. Our investigation has provided evidence that the peptide conjugated AuNPs have high in vivo mobility and targets pancreatic tumor effectively. We have also established that the EGFR-peptide-AuNP conjugates serve as better X-ray contrast agents for early detection of pancreatic cancer in mice models. The details of this comparative study will be presented in this poster

Green nanotechnology from cumin phytochemicals : generation of biocompatible gold nanoparticles

Published in final edited form as: Int J Green Nanotechnol Biomed. 2009 January 1; 1(1): B39-B52. doi:10.1080/19430850902931599.The powerful antioxidant characteristics of various phytochernicals within cumin prompted us to test their efficacy in reducing sodium tetrachloroaurate to corresponding gold nanoparticles. We, herein, report an unprecedented synthetic route that involves the production of well-defined spherical gold nanoparticles by simple mixing of cumin to an aqueous solution of sodium tetrachloro aurate. Production of gold nanoparticles in this cumin-mediated Green Nanotechnological process is achieved under biologically benign conditions. The gold nanoparticles generated through cumin-mediated process did not aggregate suggesting that the cocktail of phytochemicals including proteins serve as excellent coatings on nanoparticles and thus, provide robust shielding from aggregations. In addition, the phytochemical coatings on nanoparticles have rendered nontoxic features to these 'Green Gold Nanoparticles' as demonstrated through detailed MTT assays performed on 'normal fibroblast cells. Results of our studies presenting a new 'Nano-Naturo' connection for the production and utility of gold nanoparticles for potential applications in nanomedicine and nanotechnology are discussed in this paper.This work has been supported by the generous support from the National Institutes of Health/National Cancer Institute under the Cancer Nanotechnology Platform program (grant number: 5R01CA119412-01), NIH - 1R21CA128460-01 and University of Missouri-Research Board - Program C8761 RB 06-030