Additional file 1: Table S1. of Analysis of age-dependent trends in Ov16 IgG4 seroprevalence to onchocerciasis

Village name, number of study participants, Ov16 prevalence and MF prevalence values used to generate Fig. 4b. (DOCX 15 kb

Lateral flow test strip scanned images showing test line (‘T’, left) and control line (‘C’, right) results after using as samples the dried anti-Ov16 IgG4-positive control subjected to elevated temperature stress over time.

<p>Time zero shown indicates intensity of the starting signal prior to storage at 25°C, 45°C, and variable cycling temperature (20°C to 40°C, daily) for up to 19 months.</p

Concentration-dependent absorbance values of the ELISA platform for the anti-Ov16 human IgG4 serological assay.

<p>(A) Absorbance units versus concentration of recombinant anti-Ov16 IgG4-positive control antibody. (B) HRP ELISA and AP ELISA absorbance values from both plasma/serum and DBS samples derived from identical sources: anti-Ov16 IgG4-positive control-spiked negative human sera, dilutions of an Ov-positive pool from microfilaria-positive patient plasmas and sera, and the average of nine negative plasmas and sera (from donors that have not traveled to an Ov-endemic area).</p

Environmental conditions during surveillance activities in Togo 2013 and 2014.

<p>A. Daily temperature profiles (dark grey lines) in degrees Celsius during surveillance activities. The mean temperature is plotted with the bold red line. B. Daily % relative humidity (dark grey lines) cycles during surveillance activities. The mean relative humidity is plotted with the bold green line. C. Frequency of recorded temperature and relative humidity pairs experienced by monitors attached to microscopes used during the surveillance activities. The color scale represents the frequency of observations represented by intense red (least frequent) to intense yellow (most frequent).</p

Workflow for use of dried positive control with the Ov16 RDT.

<p>Workflow for use of dried positive control with the Ov16 RDT.</p

Dose-dependency of the intensity of the RDT (SD BIOLINE Onchocerciasis IgG4) test line result to increasing concentrations of the anti-Ov16 human IgG4 spiked into pooled normal human plasma.

<p>Ten microliters of the plasma is applied as sample and run according to manufacturer’s instructions.</p

(A) Combined inter-laboratory HRP ELISA signal profiles for anti-Ov16 human IgG4 antibody.

<p>Concentrations between 0.1ng/mL and 20ng/mL were assayed in replicate ELISA plates by four different laboratories using the same protocol and starting reagents. Each plate contained the concentration range run in duplicate wells. Different laboratories, identified as A-D, ran between three and five replicate plates. Shown is data collected from the laboratories (color-grouped) with each line representing the profile from a single plate of the averaged duplicate wells for each concentration tested. (B) Processed data representing the same data with the same color-coding as shown in 3A, but shown with absorbance values normalized to each plate’s respective optical absorbance at 2.5 ng/mL of control antibody.</p