1 research outputs found
Plasmonic Sensor Could Enable Label-Free DNA Sequencing
We demonstrated a
proof-of-principle concept of a label-free platform
that enables nucleic acid sequencing by binding methodology. The system
utilizes gold surfaces having high fidelity plasmonic nanohole arrays
which are very sensitive to minute changes of local refractive indices.
Our novel surface chemistry approach ensures accurate identification
of correct bases at individual positions along a targeted DNA sequence
on the gold surface. Binding of the correct base on the gold sensing
surface triggers strong spectral variations within the nanohole optical
response, which provides a high signal-to-noise ratio and accurate
sequence data. Integrating our label-free sequencing platform with
a lens-free imaging-based device, we reliably determined targeted
DNA sequences by monitoring the changes within the plasmonic diffraction
images. Consequently, this new label-free surface chemistry technique,
integrated with plasmonic lens-free imaging platform, will enable
monitoring multiple biomolecular binding events, which could initiate
new avenues for high-throughput nucleic acid sequencing