118 research outputs found
Comparative analysis of expression of angiogenic factors and CD44 gene in human glioma and neuroblastoma cell lines in vitro
Angiogenesis is essential for tumor growth and relies on the production of angiogenic factors. By comparative analysis using RT-PCR method of angiogenic growth factors: VEGF, bFGF, PDGF-A, angiogenin- 1 and IL-8 we established the level of expression of these genes necessary for angiogenesis in glioma and neuroblastoma cell lines. Our analyses were also extended to CD44 gene, which plays an important role in cascade of metastasis and progression of brain tumors. Significant differences in the level of gene expression of angiogenic factors and CD44 gene between the two cell lines observed throughout this study can be used as a prognostic marker for predicting clinical outcome in human brain tumors at the time of the initial staging.Angiogeneza je neophodna za rast tumora i zahteva proizvodnju angiogenih trofiÄkih faktora koji uÄestvuju u tumorogenezi. Uporednom analizom angiogenih trofiÄkih faktora: VEGF, bFGF, PDGF-A, angiogenina-1 i IL-8 pomoÄu metode RT-PCR utvrdili smo nivo ekspresije ovih gena ukljuÄenih u proces angiogeneze u Äelijskim linijama glioma i neuroblastoma. TakoÄe smo proÅ”irili analize i na CD44 gen koji igra važnu ulogu u kaskadi nastanka i progresiji metastaza tumora mozga. Dobijeni rezultati ukazuju na znaÄajnu razliku u nivou genske ekspresije angiogenih faktora i CD44 gena u ove dve Äelijske linije Äije se poreklo razlikuje ne samo po nastanku veÄ i po mestu rasejavnja metastaza. Rezultati bi mogli da posluže kao prognostiÄki faktor u prekliniÄkim i kliniÄkim istraživanjima tumora mozga od inicijalnih do terminalnih stupnjeva nastanka i terapije
6-hydroxydopamine lesions of the striatum lead to the alterations of dopamine receptor mrna in parkinsonian rats
The effects of four-site intrastriatal 6-hydroxydopamine (6-OHDA) lesions were examined in adult male rats. Five days after the lesions the animals were checked for specific rotational behavior induced by middle dose of amphetamine and the results confirmed the effectiveness of the lesions. The RNAs from the striatum were isolated at different time points after the lesion, and the RT-PCR analyse were performed for the D1 and D2 receptor mRNA. The results show a decline in the D2 receptor mRNA level (40%) at 6 h and 24 h points while this change was not observed seven days after the lesion. In contrast, no statistically significant changes in the level of the D1 receptor mRNA after the lesion at any time point were found.Ispitivani su efekti Äetiri ubodne 6-hidroksidopaminske (6-OHDA) lezije striatuma kod odraslih mužjaka pacova. Pet dana nakon lezije, životinje su testirane na specifiÄno rotaciono ponaÅ” anje pod uticajem srednje doze amfetamina i rezultati su potvrdili efikasnost lezije. RNK iz striatuma su izolovane u razliÄitim vremenskim taÄkama nakon lezije i uraÄena je RT-PCR analiza iRNK za D1 i D2 dopaminske receptore. Rezultati pokazuju smanjivanje nivoa iRNK za D2 receptor (40%) 6 h i 24 h nakon lezije, dok sedam dana nakon lezije nema promena. Za razliku od ovih rezultata, u nivou iRNK za D1 receptor ne postoje statistiÄki znaÄajne razlike u bilo kojoj vremenskoj taÄki
Changes in expression of GFAP, ApoE and APP mRNA and protein levels in the adult rat brain following cortical injury
The recovery period following cortical injury (CI) is characterized by a dynamic and highly complex interplay between beneficial and detrimental events. The aim of this study was to examine the expressions of Glial Fibrillary Acidic Protein (GFAP), Apolipoprotein E (ApoE) and Amyloid Precursor Protein (APP), all of which are involved in brain plasticity and neurodegeneration. Our results reveal that CI strongly influenced GFAP, ApoE and APP mRNA expression, as well as GFAP and ApoE protein expression. Considering the pivotal role of these proteins in the brain, the obtained results point to their potential contribution in neurodegeneration and consequent Alzheimer's disease development.Projekat ministarstva br. 17305
Comparative analysis of expression of angiogenic factors and CD44 gene in human glioma and neuroblastoma cell lines in vitro
Angiogenesis is essential for tumor growth and relies on the production of angiogenic factors. By comparative analysis using RT-PCR method of angiogenic growth factors: VEGF, bFGF, PDGF-A, angiogenin- 1 and IL-8 we established the level of expression of these genes necessary for angiogenesis in glioma and neuroblastoma cell lines. Our analyses were also extended to CD44 gene, which plays an important role in cascade of metastasis and progression of brain tumors. Significant differences in the level of gene expression of angiogenic factors and CD44 gene between the two cell lines observed throughout this study can be used as a prognostic marker for predicting clinical outcome in human brain tumors at the time of the initial staging.Angiogeneza je neophodna za rast tumora i zahteva proizvodnju angiogenih trofiÄkih faktora koji uÄestvuju u tumorogenezi. Uporednom analizom angiogenih trofiÄkih faktora: VEGF, bFGF, PDGF-A, angiogenina-1 i IL-8 pomoÄu metode RT-PCR utvrdili smo nivo ekspresije ovih gena ukljuÄenih u proces angiogeneze u Äelijskim linijama glioma i neuroblastoma. TakoÄe smo proÅ”irili analize i na CD44 gen koji igra važnu ulogu u kaskadi nastanka i progresiji metastaza tumora mozga. Dobijeni rezultati ukazuju na znaÄajnu razliku u nivou genske ekspresije angiogenih faktora i CD44 gena u ove dve Äelijske linije Äije se poreklo razlikuje ne samo po nastanku veÄ i po mestu rasejavnja metastaza. Rezultati bi mogli da posluže kao prognostiÄki faktor u prekliniÄkim i kliniÄkim istraživanjima tumora mozga od inicijalnih do terminalnih stupnjeva nastanka i terapije
Collagen type I alpha 1 gene polymorphism in premature ovarian failure
Introduction. Premature ovarian failure (POF) is characterized by amenorrhea, hypergonadotropism and hypoestrogenism in women bellow 40 years. Osteoporosis is one of the late complications of POF. Objective. To correlate collagen type I alpha1 (COLIA1) gene polymorphism with bone mineral density (BMD) in women with POF. Methods. We determined the COLIA1 genotypes SS, Ss, ss in 66 women with POF. Single nucleotide polymorphism (G to T substitution) within the Sp 1-binding site in the first intron of the COLIA1 gene was assessed by polymerase chain reaction (PCR) followed by single-stranded conformation polymorphism (SSCP) analysis. Bone mineral density (BMD) was measured at the lumbar spine region by dual X-ray absorptiometry. Statistics: Kruskal-Wallis ANOVA, Chisquare test, Spearman correlation test. Results. The relative distribution of COLIA1 genotype alleles was SS - 54.4%, Ss - 41.0% and ss - 4.5%. No significant differences were found between genotype groups in body mass index, age, duration of amenorrhea or BMD. A significant positive correlation was observed between BMI and parity. Conclusion. The COLIA1 gene is just one of many genes influencing bone characteristics. It may act as a marker for differences in bone quantity and quality, bone fragility and accelerated bone loss in older women. However, in young women with POF, COLIA1 cannot identify those at higher risk for osteoporosis.Uvod. Prevremena insuficijencija jajnika (PIJ) se odlikuje amenorejom, hipergonadotropizmom i hipoestrogenijom kod žena mlaÄih od 40 godina. Osteoporoza je kasna komplikacija ovog stanja. Cilja rada. Cilj istraživanja je bio da se uporede genski polimorfizam kolagena tip I alfa 1 (COLIA1) sa gustinom koÅ”tane mase kod žena sa PIJ. Metode rada. OdreÄivan je COLIA1 genotip SS, Ss i ss kod 66 žena sa PIJ pomoÄu eseja za reakciju lanÄanog umnožavanja DNK (engl. polymerase chain reaction - PCR). Polimorfizam jednog nukleotida (zamena G u T) u okviru Sp1 vezujuÄeg mesta u prvom intronu gena COLIA1 odreÄivan je primenom PCR, nakon Äega se pristupilo analizi konformacionog polimorfizma. Gustina koÅ”tane mase je merena na nivou lumbalnog dela kiÄme pomoÄu apsorpciometrije. Hormonske analize za folikulostimuliÅ”uÄi hormon, luteinizirajuÄi hormon, estradiol, prolaktin, progesteron i testosteron uraÄene su primenom metoda RIA. Pri statistiÄkoj obradi podataka koriÅ”Äeni su: Kraskal-Volisov (Kruskal-Wallis) ANOVA test, Ļ2-test i Spirmanov (Spearman) test korelacije. Rezultati. Relativna distribucija alela COLIA1 genotipa bila je: SS 54,4%, Ss 41,0% i ss 4,5%. Nije utvrÄena znaÄajna razlika izmeÄu grupa prema genotipu za gustinu koÅ”tane mase, starost ispitanica, period amenoreje ili indeks telesne mase žena. ZnaÄajna pozitivna korelacija je uoÄena za indeks telesne mase i paritet. ZakljuÄak. COLIA1 je samo jedan od mnogih gena koji utiÄu na karakteristike kosti. Kod žena starije životne dobi on može biti marker kvaliteta, kvantiteta i osetljivosti kosti. Kod mladih žena sa PIJ COLIA1 ne može da ukaže na one žene kod kojih postoji veÄi rizik za nastanak osteoporoze.Projekat ministarstva br. ON 17305
Apoptotic clearance in rabbits with experimental pulmonary emphysema
In order to better understand pathogenesis of pulmonary emphysema, the model of experimentally induced pulmonary emphysema in Chinchilla rabbits was used for the estimation of apoptotic clearance of pulmonary tissue. Bronchoalveolar lavage was performed in three groups of animals: experimental group-E on hypercholesterolemic diet (4% edible oil solution of crystalline cholesterol), control group-C1 on standard diet for that animal species and animals on oily diet-C2. Apoptotic detection in cytocentrifuge preparations of lung washings was evaluated by in situ TUNEL. The property of alveolar macrophages to engulf apoptotic cells was estimated by light microscopy including 300 features (related subsequent steps: adsorption, internalization and intracellular processing of free apoptotic bodies) and was evaluated by scoring and indexing method. Internalization of apoptotic bodies by alveolar macrophages, as well as free apoptotic bodies were decreased in E compared to both C1 and C2 group (p<0.01 and p<0.05 respectively). Intracellular processing of apoptotic bodies by alveolar macrophages is significantly decreased in C2 in comparison with E (p<0.05) and C1 group (p<0.01). Apoptotic capacity of pulmonary tissue is significantly decreased in C2 in comparison with C1 group (p<0.01). The results implicate that immuno-metabolic competence of pulmonary tissue might be essentially associated with tissue remodeling in pulmonary emphysema.U cilju boljeg razumevanja patogeneze pluÄnog emfizema, u radu je koriÅ”Ä en eksperimentalni model emfizema pluÄa na ÄinÄila kuniÄima za procenu apoptotskog kapaciteta pluÄnoga tkiva. Bronholaveolarna lavaža je uraÄena na tri grupe životinja: eksperimentalnoj grupi-E na hiperholesterolskoj dijeti (4% uljani rastvor kristalnog holesterola), kontrolnoj grupi-C1 na standardnoj dijeti za tu životinjsku vrstu i grupi životinja na uljanoj dijeti-C2. OdreÄivanje apoptotskih parametara cito-centrifužnih preparata bronhoalveolarnog lavata vrÅ”eno je posle bojenja preparata TUNEL in situ citohemijskim metodom. Sposobnost alveolarnih makrofaga da odstrane apoptotske Äelije fagocitozom procenjivana je svetlosnom mikroskopijom na 300 prikaza po preparatu (prikazi ukljuÄuju: adsorpciju, internalizaciju i intracelularno procesiranje apoptotskih tela) i evaluirana metodom indeksiranja i skora. Internalizacija apoptotskih tela alveolarnim makrofazima, kao i relativni procenat slobodnih apoptotskih tela, bili su signifikantno smanjeni u E grupi poredeÄi sa C1 (p<0.01) i C2 grupom (p<0.05). Intracelularno procesiranje apoptotskih tela alveolarnim makrofazima bilo je signifikantno smanjeno u C2 u odnosu na E (p<0.05) i C1 grupu (p<0.01). Apoptotski kapacitet tkiva pluÄa bio je signifikantno smanjen u C2 u poreÄenju sa C1 grupom (p<0.01). Ovi rezultati ukazuju da imuno-metaboliÄka kompetentnost pluÄnog tkiva može biti suÅ”tinski povezana sa remodelovanjem tkiva pluÄa u eksperimentalnom emfizemu pluÄa.nul
Supplementary material for the article: MiloÅ”ev, M. Z.; JakovljeviÄ, K.; JoksoviÄ, M. D.; StanojkoviÄ, T.; MatiÄ, I. Z.; PeroviÄ, M.; TeÅ”iÄ, V.; Kanazir, S.; MladenoviÄ, M.; RodiÄ, M. V.; et al. Mannich Bases of 1,2,4-Triazole3-Thione Containing Adamantane Moiety: Synthesis, Preliminary Anticancer Evaluation, and Molecular Modeling Studies. Chemical Biology and Drug Design 2017, 89 (6), 943ā952. https://doi.org/10.1111/cbdd.12920
Supporting Information: [https://doi.org/10.1111/cbdd.12920]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2482]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3221
Supplementary material for the article: MiloÅ”ev, M. Z.; JakovljeviÄ, K.; JoksoviÄ, M. D.; StanojkoviÄ, T.; MatiÄ, I. Z.; PeroviÄ, M.; TeÅ”iÄ, V.; Kanazir, S.; MladenoviÄ, M.; RodiÄ, M. V.; et al. Mannich Bases of 1,2,4-Triazole3-Thione Containing Adamantane Moiety: Synthesis, Preliminary Anticancer Evaluation, and Molecular Modeling Studies. Chemical Biology and Drug Design 2017, 89 (6), 943ā952. https://doi.org/10.1111/cbdd.12920
Supporting Information: [https://doi.org/10.1111/cbdd.12920]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2482]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3221
Time-dependent Stochastic Modeling of Solar Active Region Energy
A time-dependent model for the energy of a flaring solar active region is
presented based on a stochastic jump-transition model (Wheatland and Glukhov
1998; Wheatland 2008; Wheatland 2009). The magnetic free energy of the model
active region varies in time due to a prescribed (deterministic) rate of energy
input and prescribed (random) flare jumps downwards in energy. The model has
been shown to reproduce observed flare statistics, for specific
time-independent choices for the energy input and flare transition rates.
However, many solar active regions exhibit time variation in flare
productivity, as exemplified by NOAA active region AR 11029 (Wheatland 2010).
In this case a time-dependent model is needed. Time variation is incorporated
for two cases: 1. a step change in the rates of flare jumps; and 2. a step
change in the rate of energy supply to the system. Analytic arguments are
presented describing the qualitative behavior of the system in the two cases.
In each case the system adjusts by shifting to a new stationary state over a
relaxation time which is estimated analytically. The new model retains
flare-like event statistics. In each case the frequency-energy distribution is
a power law for flare energies less than a time-dependent rollover set by the
largest energy the system is likely to attain at a given time. For Case 1, the
model exhibits a double exponential waiting-time distribution, corresponding to
flaring at a constant mean rate during two intervals (before and after the step
change), if the average energy of the system is large. For Case 2 the
waiting-time distribution is a simple exponential, again provided the average
energy of the system is large. Monte Carlo simulations of Case~1 are presented
which confirm the analytic estimates. The simulation results provide a
qualitative model for observed flare statistics in active region AR 11029.Comment: 25 pages, 9 figure
Changes in expression of GFAP, ApoE and APP mRNA and protein levels in the adult rat brain following cortical injury
The recovery period following cortical injury (CI) is characterized by a dynamic and highly complex interplay between beneficial and detrimental events. The aim of this study was to examine the expressions of Glial Fibrillary Acidic Protein (GFAP), Apolipoprotein E (ApoE) and Amyloid Precursor Protein (APP), all of which are involved in brain plasticity and neurodegeneration. Our results reveal that CI strongly influenced GFAP, ApoE and APP mRNA expression, as well as GFAP and ApoE protein expression. Considering the pivotal role of these proteins in the brain, the obtained results point to their potential contribution in neurodegeneration and consequent Alzheimer's disease development.Projekat ministarstva br. 17305
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