Effect of Various Nutritional Supplements on Hyaluronic Acid Production

Effect of various nutritional supplementations on production of hyaluronic acid (HA) using Streptococcus equi subsp. zooepidemicus ATCC 39920 was evaluated at shake flask. Among the different carbon sources used, maltodextin showed HA of 0.9 g/L followed by 0.7 g/L for dextrose. L-arginine HCl at 2.0 g/L increased the yield of HA from 0.7 g/L to 0.85 g/L. L-arginine and L-glutamine showed the yield of HA of 0.7 g/L and 1.0 g/L, respectively at 2.0 g/L concentration. L-glutamine (2.0 g/L) and uridine (0.75 g/L) showed HA yield of 0.8 and 1.045 g/L, respectively. Addition of sodium glucuronate at 0.25 g/L concentration at 8 h, the yield of HA was increased from 0.7 g/L to 0.85 g/L. Pantothenic acid at concentration of 8.33 mg/L supplemented to the P10 medium, yield of 0.87 g/L of HA was obtained. Active dry yeast at 5.0 g/L increased yield of HA from 0.7 g/L to 0.85 g/L. Tween 80 at higher concentration reduced HA production. The addition of MgSO4.7H2O at 1.0 g/L improved the production of HA. Other metal ions like manganese, ferrous, copper, and zinc have no significant effect on HA production. Thus, it has been found that various nutritional supplements have effect on production of HA using S. equi subsp. zooepidemicus ATCC 39920

Effect of Various Physical Parameters and Statistical Medium Optimization on Production of Hyaluronic Acid Using S. Equi Subsp. Zooepidemicus ATCC 39920

It has been shown that initial conditions for bacterial cultivation are extremely important for the successful production of hyaluronic acid (HA) by fermentation. We investigated several physical parameters that affect productivity of HA under shake flask. i.e. transfer criteria of seed, agitation and aeration of fermentation flasks. Among the various physical parameters studied, inoculum age of 8-10 h, pH 6.4, optical density (600 nm) 2.0 and 3% level inoculum transfer found to be optimum. After inoculating with Streptococcus equi subsp. zooepidemicus ATCC 39920, the temperature 37 0C and 90 rpm found optimum during growth as well as for the HA production. The fractional factorial design of six factors with two levels showed yeast extract, potassium dihydrogen phosphate and sodium bicarbonate as significant model terms. The factor potassium dihydrogen phosphate was relatively more significant than yeast extract.

ANTIFUNGAL ACTIVITY OF LIPASE MODIFIED FLAVONOIDS FROM CITRUS LIMETTA

Objective: The aim of the present study is to bring about enzymatic modification of flavonoids from Citrus limetta in order to increase their antifungal activity. Methods: Methanolic extraction of flavonoids from citrus peels was carried out and their presence was confirmed by various tests. Crude extract of flavonoids was subjected to enzymatic modification by enzyme lipase in presence of acyl donor tributyrin in order to bring about esterification. Esterification was confirmed using IR spectroscopy and sodium hydroxide- phenolphthalein test. Antifungal activity of the treated sample and control sample were tested against Candida albicans and compared with fluconazole by Kirby Bauer disk diffusion method. Results: Methanolic extract showed the presence of flavonoids on carrying out various test. While testing enzymatic modification, IR spectra did not show a significant difference between treated and control; however, enhanced esterification was confirmed by sodium hydroxide-phenolphthalein test. On carrying out antifungal activity, treated samples showed a 56.52% increase in zone of inhibition against Canidida albicans as compared to the control and was also found to be more in comparison to fluconazole. Conclusion: The results signify that enzymatic modification increases the antifungal activity of flavonoids from Citrus limetta

Record catch of rock perch, Epinephelus fasciatus by bottom trawling off Ratnagiri

A productive fishing ground for rock perch (Epinephelus fasciatus) was located off Ratnagiri between 17 degree 00' to 17 degree 10' N latitude and 72 degree 20' to 72 degree 30' E longitude, around a depth of 96m during November, 1983. An average catch of 2 ton/hr. was recorded. Length frequency studies of E. fasciatus indicate the probability of a nursery ground consisting of three brood stocks. Hydrographic studies of the ground revealed that the bottom water was oxygen deficient below a strong thermocline extending from 15-75 m depth with the recorded bottom temperature amounting to 20.5 degree C

Rupture uterus: a prospective observational study of 25 consecutive cases in a tertiary referral centre in South India

Background: Uterine rupture in pregnancy is a rare though catastrophic complication with a high incidence of foetal and maternal morbidity. It appeared to be on the decline in recent times, prompting us to analyse the clinical picture now. The objective of the study was to study the risk factors, clinical profile and consequences of uterine rupture on maternal and perinatal outcome.Methods: A prospective observational study of 25 consecutive cases of uterine rupture was carried out between July 2013 and October 2015. All the cases of rupture uterus either referred or diagnosed after admission were included. Detailed demographic data, past and present obstetric events, time taken to reach the referral centre, the reasons for any delay, mode of presentation, type of rupture, management and maternal and foetal outcomes were analysed.Results: The incidence of rupture was 1 in 915 deliveries. Seventy-two per cent of them were referred cases. Average time to reach from referral centre was 4.3 hours, the common reasons for delay being patient factors (33%), transport problems (38%) and delayed referrals (22%). Previous caesarean section was the most common predisposing factor (56%). Sixty-four per cent of the ruptures were diagnosed clinically. Eighty-four per cent of the ruptures were complete. Sub-total hysterectomy was carried out in 14 (56%) patients. Internal iliac artery ligation (20%) and bladder repair (8%) were sometimes required. All women required blood transfusion.  Perinatal mortality was seen in 76%. There was no maternal mortality.Conclusions: Uterine rupture is a cause of severe acute maternal morbidity and very high perinatal mortality.  Delay in referral and diagnosis can lead to significant maternal morbidity and hysterectomy

Rare case report of metastatic cervical carcinoma

Although carcinoma of cervix is among the most common malignancies in women, cutaneous metastasis is very unusual. Here we report a case of a parous woman diagnosed with adenocarcinoma of cervix who did not undergo chemotherapy in spite of being advised after initial surgery, presenting with cutaneous metastasis at the operative scar site 2 years later

Pregnancy outcome in a rare case of complex cyanotic congenital heart disease

A 28 year old primigravida with the very rare congenital heart condition of single ventricle with single atrium presented with 34 weeks gestation, and underwent caesarean section with minimal morbidity. As the condition is not known for survival into adulthood, the carrying of a pregnancy successfully to near term makes this an even rarer case