Prospects of new chickpea varieties in Andhra Pradesh

Andhra Pradesh is an important chickpea growing state in southern India, with spectacular increase in chickpea area from 120,000 ha in 1997/98 to 638,000 ha in 2007/08. The chickpea revolution in Andhra Pradesh has improved the prospects of many resource-poor, small land holding and rainfed farmers of Andhra Pradesh. However, the growing season of chickpea in Andhra Pradesh is warm and short (90-110 days), and drought is the foremost factor responsible for significant yield losses. Rainfall in major chickpea-growing regions is quite uncertain and erratic, resulting in poor yields. The Regional Agricultural Research Station of Acharya N G Ranga Agricultural University, Nandyal, Andhra Pradesh, India is the lead centre responsible for location-specific research in chickpea in Andhra Pradesh. With support from ICRISAT and ICAR, the centre has initiated crop improvement programmes during 2004 and has released four promising chickpea varieties for commercial cultivation. Three desi varieties viz., Nandyal Sanaga 1(NBeG 3), Dheera (NBeG 47), and Nandyal Gram 49 (NBeG 49) released for Andhra Pradesh and one large-seeded kabuli Nandyal Gram 119 (NBeG 119) released for the southern zone comprising Andhra Pradesh, Karnataka and Tamil Nadu, are cutting across chickpea growing regions of Andhra Pradesh. Nandyal Sanaga 1, released in 2012, is a bold-seeded desi variety tolerant to drought and heat; Dheera released during 2015 is also a desi variety and the first of its kind in India, suitable for mechanical harvesting. Nandyal Gram 49 released during 2016 is a highyielding desi variety with attractive seeds; whereas Nandyal Gram 119 is early bold-seeded kabuli variety released during 2015. These varieties have clearly demonstrated their advantage (10%-15 % increase over popular varieties of the tract) in farmers’ holdings in large-scale demonstrations and are being preferred by farmers of not only Andhra Pradesh, but also Karnataka, Tamil Nadu, Odisha and Maharastra. Efforts are underway to promote large-scale adoption of these varieties to maximize long term productivity of chickpeas in rainfed vertisols

Consortium on Vulnerability to Externalizing Disorders and Addictions (cVEDA):A developmental cohort study protocol

Background: Low and middle-income countries like India with a large youth population experience a different environment from that of high-income countries. The Consortium on Vulnerability to Externalizing Disorders and Addictions (cVEDA), based in India, aims to examine environmental influences on genomic variations, neurodevelopmental trajectories and vulnerability to psychopathology, with a focus on externalizing disorders. Methods: cVEDA is a longitudinal cohort study, with planned missingness design for yearly follow-up. Participants have been recruited from multi-site tertiary care mental health settings, local communities, schools and colleges. 10,000 individuals between 6 and 23 years of age, of all genders, representing five geographically, ethnically, and socio-culturally distinct regions in India, and exposures to variations in early life adversity (psychosocial, nutritional, toxic exposures, slum-habitats, socio-political conflicts, urban/rural living, mental illness in the family) have been assessed using age-appropriate instruments to capture socio-demographic information, temperament, environmental exposures, parenting, psychiatric morbidity, and neuropsychological functioning. Blood/saliva and urine samples have been collected for genetic, epigenetic and toxicological (heavy metals, volatile organic compounds) studies. Structural (T1, T2, DTI) and functional (resting state fMRI) MRI brain scans have been performed on approximately 15% of the individuals. All data and biological samples are maintained in a databank and biobank, respectively. Discussion: The cVEDA has established the largest neurodevelopmental database in India, comparable to global datasets, with detailed environmental characterization. This should permit identification of environmental and genetic vulnerabilities to psychopathology within a developmental framework. Neuroimaging and neuropsychological data from this study are already yielding insights on brain growth and maturation patterns.</p

Positive feedback and noise activate the stringent response regulator Rel in mycobacteria

Phenotypic heterogeneity in an isogenic, microbial population enables a subset of the population to persist under stress. In mycobacteria, stresses like nutrient and oxygen deprivation activate the stress response pathway involving the two-component system MprAB and the sigma factor, SigE. SigE in turn activates the expression of the stringent response regulator, rel. The enzyme polyphosphate kinase 1 (PPK1) regulates this pathway by synthesizing polyphosphate required for the activation of MprB. The precise manner in which only a subpopulation of bacterial cells develops persistence, remains unknown. Rel is required for mycobacterial persistence. Here we show that the distribution of rel expression levels in a growing population of mycobacteria is bimodal with two distinct peaks corresponding to low (L) and high (H) expression states, and further establish that a positive feedback loop involving the mprAB operon along with stochastic gene expression are responsible for the phenotypic heterogeneity. Combining single cell analysis by flow cytometry with theoretical modeling, we observe that during growth, noise-driven transitions take a subpopulation of cells from the L to the H state within a "window of opportunity" in time preceding the stationary phase. We find evidence of hysteresis in the expression of rel in response to changing concentrations of PPK1. Our results provide, for the first time, evidence that bistability and stochastic gene expression could be important for the development of "heterogeneity with an advantage" in mycobacteria.Comment: Accepted for publication in PLoS On

Novel Role of Phosphorylation-Dependent Interaction between FtsZ and FipA in Mycobacterial Cell Division

The bacterial divisome is a multiprotein complex. Specific protein-protein interactions specify whether cell division occurs optimally, or whether division is arrested. Little is known about these protein-protein interactions and their regulation in mycobacteria. We have investigated the interrelationship between the products of the Mycobacterium tuberculosis gene cluster Rv0014c-Rv0019c, namely PknA (encoded by Rv0014c) and FtsZ-interacting protein A, FipA (encoded by Rv0019c) and the products of the division cell wall (dcw) cluster, namely FtsZ and FtsQ. M. smegmatis strains depleted in components of the two gene clusters have been complemented with orthologs of the respective genes of M. tuberculosis. Here we identify FipA as an interacting partner of FtsZ and FtsQ and establish that PknA-dependent phosphorylation of FipA on T77 and FtsZ on T343 is required for cell division under oxidative stress. A fipA knockout strain of M. smegmatis is less capable of withstanding oxidative stress than the wild type and showed elongation of cells due to a defect in septum formation. Localization of FtsQ, FtsZ and FipA at mid-cell was also compromised. Growth and survival defects under oxidative stress could be functionally complemented by fipA of M. tuberculosis but not its T77A mutant. Merodiploid strains of M. smegmatis expressing the FtsZ(T343A) showed inhibition of FtsZ-FipA interaction and Z ring formation under oxidative stress. Knockdown of FipA led to elongation of M. tuberculosis cells grown in macrophages and reduced intramacrophage growth. These data reveal a novel role of phosphorylation-dependent protein-protein interactions involving FipA, in the sustenance of mycobacterial cell division under oxidative stress

AI is a viable alternative to high throughput screening: a 318-target study

: High throughput screening (HTS) is routinely used to identify bioactive small molecules. This requires physical compounds, which limits coverage of accessible chemical space. Computational approaches combined with vast on-demand chemical libraries can access far greater chemical space, provided that the predictive accuracy is sufficient to identify useful molecules. Through the largest and most diverse virtual HTS campaign reported to date, comprising 318 individual projects, we demonstrate that our AtomNet® convolutional neural network successfully finds novel hits across every major therapeutic area and protein class. We address historical limitations of computational screening by demonstrating success for target proteins without known binders, high-quality X-ray crystal structures, or manual cherry-picking of compounds. We show that the molecules selected by the AtomNet® model are novel drug-like scaffolds rather than minor modifications to known bioactive compounds. Our empirical results suggest that computational methods can substantially replace HTS as the first step of small-molecule drug discovery

Everyday networks, politics, and inequalities in post-tsunami recovery: fisher livelihoods in South Sri Lanka

The aim of this thesis is to explore how livelihoods are recovering in the aftermath of the 2004 tsunami in Sri Lanka through the lens of the Sustainable Livelihoods Framework and the social networks approach—methods of inquiry that have gained considerable impetus in livelihoods research. The study is conducted with reference to two tsunami-affected fisher villages in the Hambantota District, Southern Province. It employs a qualitative ethnographic methodology that examines narratives emerging from households, local officials of government and non-government organizations, office bearers of community-based organizations, local politicians, village leaders, and key informants. Focus is on evaluating how particular roles, activities, and behaviour are given importance by these groups in specific post-tsunami contexts and how these aspects relate to broader conceptualizations of social networks, informal politics, social inequality, and ethnographic research in South Asia. The findings support four major contributions to the literature. First, social networks are significant as an object of study and a method of inquiry in understanding livelihoods post-disaster. Second, paying heed to varied forms of informal politics is critical in post-disaster analyses. Third, the concept of intersectionality can extend and improve upon prevailing approaches to social inequality in disaster recovery. Fourth, ethnographic research is valuable for understanding everyday networks, informal politics, and change in South Asia. Collectively, these findings present a human geography of post-tsunami livelihoods in Sri Lanka, where networks, politics, and inequalities, which form an essential part of everyday livelihoods, have been reproduced in disaster recovery. The thesis constitutes a means of offering expertise in the sphere of development practice, highlighting internal differentiation in access to aid as a key issue that needs to be identified and systematically addressed by policymakers and practitioners

A study of absconded mental patients

Briefly reports a follow-up study of 19 mental patients who "absconded" in 1973-1974 from the Government Mental Hospital in Madras. Keywords: Absconded, mental patient

Everyday networks, politics, and inequalities in post-tsunami recovery : fisher livelihoods in South Sri Lanka

The aim of this thesis is to explore how livelihoods are recovering in the aftermath of the 2004 tsunami in Sri Lanka through the lens of the Sustainable Livelihoods Framework and the social networks approach—methods of inquiry that have gained considerable impetus in livelihoods research. The study is conducted with reference to two tsunami-affected fisher villages in the Hambantota District, Southern Province. It employs a qualitative ethnographic methodology that examines narratives emerging from households, local officials of government and non-government organizations, office bearers of community-based organizations, local politicians, village leaders, and key informants. Focus is on evaluating how particular roles, activities, and behaviour are given importance by these groups in specific post-tsunami contexts and how these aspects relate to broader conceptualizations of social networks, informal politics, social inequality, and ethnographic research in South Asia. The findings support four major contributions to the literature. First, social networks are significant as an object of study and a method of inquiry in understanding livelihoods post-disaster. Second, paying heed to varied forms of informal politics is critical in post-disaster analyses. Third, the concept of intersectionality can extend and improve upon prevailing approaches to social inequality in disaster recovery. Fourth, ethnographic research is valuable for understanding everyday networks, informal politics, and change in South Asia. Collectively, these findings present a human geography of post-tsunami livelihoods in Sri Lanka, where networks, politics, and inequalities, which form an essential part of everyday livelihoods, have been reproduced in disaster recovery. The thesis constitutes a means of offering expertise in the sphere of development practice, highlighting internal differentiation in access to aid as a key issue that needs to be identified and systematically addressed by policymakers and practitioners.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

CONTROL OF PULSE BEETLE CALLOSOBRUCHUS MACULATUS USING EDIBLE PLANT LEAF EXTRACT

Leaf extracts of five edible plants were tested against notorious pulse beetle Callosobruchus maculatus infesting cowpea, Vigna unguiculata seeds. C. maculatus was introduced into plastic containers stored with seeds dipped in 5% and 10% concentration of dry and fresh leaf extracts. Number of eggs laid, adult emergence arid seed weight loss were minimum in Mentha arvensis, Sesbania glandiflora and Ocimum sanctum treatments than control and other treatments with Murraya koenigi and Coriandrum sativum