FREQUENCY AND VIABILITY OF DIPLOID AND HAPLOID MALES OFFSPRINGS OF MATED FEMALES OF SOLITARY ENDOPARASITOID (DIADROMUS PULCHELLUS ) ( ICHNEUMONIDAE) .

Sex determination in the order Hymenoptera is based on arrhenotoky, hymenopteran males are usually haploid and females diploid. Males of the Ichneumonidae Diadromus pulchellus , solitary endoparasitoid of A. assectella pupae are normally haploid, but diploid males are present in a natural population and can be obtained in a experimental population. The future of an ovocyte laid by mated females of the solitary endoparasitoid Diadromus pulchellus was characterised by 6 probabilities related to the sex and the development of the ovocyte. The probabilities of fertilisation of female ovocyte (k1) or non-fertilisation (k3) showed that an inseminated female functioned as a unmated female for half of the time (since k1= 0.492 and k3 = 0.455) with the probability of fertilisation of male ovocyte (k2 = 0.053).The survival probabilities of each type of ovocyte showed that an ovocyte had a high probability of developing up to the adult stage, although the difference between the calculated sex ratios at laying (males / females = 1.032) and at emergence (0.90) revealed a slight reduction in the number of haploid sons. The probabilities of fertilisation and of viability of all the ovocytes laid by each of the 33 mated females were analysed by an ascending hierarchical classification of Euclidean distances and by an analysis of their principal components. The 33 mothers were distributed into 4 distinct sub-groups characterised by a sex ratio varying from an exclusive presence of females to an exclusive presence of males. Our hypothesis was that this distribution in 4 sub-sets could not simply result from the random nature of the sample

Reproductive capacity of females Eupelmus vuilleti (Eupelmidae) inseminated by hyperparasitoid males developed upon the primary parasitoid Dinarmus basalis (Pteromalidae).

Eupelmus vuilleti is a primary and solitary ectoparasitoid of the larval stages of Bruchids (Callosobruchus maculatus, Bruchidius atrolineatus). In a context of intense competition for healthy hosts, E. vuilleti displays ovicide and larvicide behaviours towards the Pteromalid D. basalis during its development (kleptoparasitism), and in an extreme expression of kleptoparasitism the E. vuilleti females hyperparasitize the final larval stage (L5 stage) of D. basalis. In this study, we compared the variability of reproductive success in males that had developed in the context of hyperparasitism to that in males that had developed on primary hosts. The adaptation capacity of the males when 24 h old was analysed in terms of their weight, of the quantity of spermatozoids stored in the seminal vesicles, of the quality of insemination determined from the quantity of spermatozoids stored in the spermatheca of the females after the first mating, and of the number of daughters produced. Adults of E. vuilleti, the larvae of which had developed as hyperparasitoids, are smaller than those that have developed on primary hosts, but they keep all the abilities required to parasite a population of primary hosts once the competitive pressure is reduced