Органолептичні показники та харчова цінність арахісу смаженого із мережі готельно-рестораних комплексів Київщини

Foods must contain all the nutrients necessary to maintain human health, development and life. These components include protein, fats, carbohydrates, vitamins, minerals and other biologically active substances, which must be in sufficient quantity and ensure the proper functioning of the body. One such food that contains significant amounts of nutrients is peanuts. Peanuts are a source of protein, carbohydrates, fats, fiber, trace elements and vitamins. The nutrients of the product are easily absorbed. The article presents the results of a study of the nutritional value of roasted salted peanuts of various manufacturers, which are used for consumption in hotel and restaurant complexes in the Kiev region. The studies were carried out in the laboratory of the State Research Control Institute of Veterinary Drugs and Feed Additives (Lviv). Nutritional value was determined according to different methods, which are intended for each indicator, and also used statistical methods. According to the research results, it was revealed that the content of moisture, protein, fat and fiber in peanuts Big Bo, Felix Peanuts and Cossack Slava ranges, respectively, in the range from 0.73 to 1.37 %, from 26.3 to 26.9, from 46.01 to 48.6 g/100 g of product and 3.56 to 3.92 %. According to organoleptic parameters, prototypes of peanuts from different manufacturers meet the standard requirements. Promising research is the study of trace elements in roasted salted peanuts from various manufacturers.Харчові продукти мають містити усі поживні речовини, необхідні для підтримання здоров’я, розвитку та життєдіяльності людини. До таких компонентів належать протеїн, жири, вуглеводи, вітаміни, мінеральні речовини та інші біологічно активні речовини, які повинні бути в достатній кількість та забезпечувати належне функціонування організму. Одним із таких харчових продуктів, який містить значну кількість поживних речовин є арахіс. Арахіс є джерелом білка, вуглеводів, жирів, клітковини, мікроелементів та вітамінів. Поживні речовини продукту легко засвоюються. У статті представлені результати дослідження поживної цінності арахісу смаженого солоного різних виробників, які використовують для вживання в готельно-рестораних комплексах Київщини. Дослідження проводили в умовах лабораторії Державного науково-дослідного контрольного інституту ветпрепаратів та кормових добавок (м. Львів). Поживну цінність визначали за різними методиками, які призначені для кожного показника, а також використовували статистичні методики. За результатами досліджень виявлено, що вміст вологи, білка, жиру та клітковини у арахісах Big Boв, Felix Peanuts та Козацька Слава коливається, відповідно, в межах від 0,73 до 1,37 %, від 26,3 до 26,9, від 46,01 до 48,6 г/100 г продукту та від 3,56 до 3,92 %. За органолептичними показниками дослідні зразки арахісу різних виробників відповідають стандартним вимогам. Перспективними дослідженнями є вивчення мікроелементів в арахісі жареному солоному різних виробників

Digital PCR provides sensitive and absolute calibration for high throughput sequencing

<p>Abstract</p> <p>Background</p> <p>Next-generation DNA sequencing on the 454, Solexa, and SOLiD platforms requires absolute calibration of the number of molecules to be sequenced. This requirement has two unfavorable consequences. First, large amounts of sample-typically micrograms-are needed for library preparation, thereby limiting the scope of samples which can be sequenced. For many applications, including metagenomics and the sequencing of ancient, forensic, and clinical samples, the quantity of input DNA can be critically limiting. Second, each library requires a titration sequencing run, thereby increasing the cost and lowering the throughput of sequencing.</p> <p>Results</p> <p>We demonstrate the use of digital PCR to accurately quantify 454 and Solexa sequencing libraries, enabling the preparation of sequencing libraries from nanogram quantities of input material while eliminating costly and time-consuming titration runs of the sequencer. We successfully sequenced low-nanogram scale bacterial and mammalian DNA samples on the 454 FLX and Solexa DNA sequencing platforms. This study is the first to definitively demonstrate the successful sequencing of picogram quantities of input DNA on the 454 platform, reducing the sample requirement more than 1000-fold without pre-amplification and the associated bias and reduction in library depth.</p> <p>Conclusion</p> <p>The digital PCR assay allows absolute quantification of sequencing libraries, eliminates uncertainties associated with the construction and application of standard curves to PCR-based quantification, and with a coefficient of variation close to 10%, is sufficiently precise to enable direct sequencing without titration runs.</p

The stages of rendering of medical aid to patients with disorders of the veins

The article presents the results of analysis of organization of medical aid to patients with disorders of the veins in the Orenburg region. The insufficient provision of population with physicians, surgeons (10 thousand to 1,37) and cardiovascular surgeons (0,15 per 10 thousand), which is lower than the national average and, as a consequence of the low staffing data specialists in the departments of vascular surgery. Among the organizational problems of medical care are leading to the absence of a day hospital for surgical treatment of this disease; not enough widespread introduction of less traumatic methods of surgical treatment and the low percentage of patients in the departments of vascular surgery on the background of their high average duration of hospital stay. For changes and improvements suggested are the stages of rendering of medical aid to this category of patients.В статье представлены результаты анализа состояния организации медицинской помощи пациентам с патологией вен в Оренбургской области. Установлена недостаточная обеспеченность населения врачами-хирургами (1,37 на 10 тыс.) и сердечно-сосудистыми хирургами (0,15 на 10 тыс.), что привело к низкой укомплектованности отделений сосудистой хирургии данными специалистами. Среди организационных проблем оказания медицинской помощи пациентам с патологией вен ведущими являются: отсутствие в регионе дневного стационара для хирургического лечения данной патологии; недостаточно широкое внедрение малотравматичных методов хирургического лечения; низкая доля пациентов с венозной патологией в отделениях сосудистой хирургии. Для изменения и улучшения ситуации разработаны этапы оказания медицинской помощи данной категории пациентов

Movement Protein Pns6 of Rice dwarf phytoreovirus Has Both ATPase and RNA Binding Activities

Cell-to-cell movement is essential for plant viruses to systemically infect host plants. Plant viruses encode movement proteins (MP) to facilitate such movement. Unlike the well-characterized MPs of DNA viruses and single-stranded RNA (ssRNA) viruses, knowledge of the functional mechanisms of MPs encoded by double-stranded RNA (dsRNA) viruses is very limited. In particular, many studied MPs of DNA and ssRNA viruses bind non-specifically ssRNAs, leading to models in which ribonucleoprotein complexes (RNPs) move from cell to cell. Thus, it will be of special interest to determine whether MPs of dsRNA viruses interact with genomic dsRNAs or their derivative sRNAs. To this end, we studied the biochemical functions of MP Pns6 of Rice dwarf phytoreovirus (RDV), a member of Phytoreovirus that contains a 12-segmented dsRNA genome. We report here that Pns6 binds both dsRNAs and ssRNAs. Intriguingly, Pns6 exhibits non-sequence specificity for dsRNA but shows preference for ssRNA sequences derived from the conserved genomic 5′- and 3′- terminal consensus sequences of RDV. Furthermore, Pns6 exhibits magnesium-dependent ATPase activities. Mutagenesis identified the RNA binding and ATPase activity sites of Pns6 at the N- and C-termini, respectively. Our results uncovered the novel property of a viral MP in differentially recognizing dsRNA and ssRNA and establish a biochemical basis to enable further studies on the mechanisms of dsRNA viral MP functions

Adipose-Derived Stem Cells Stimulate Regeneration of Peripheral Nerves: BDNF Secreted by These Cells Promotes Nerve Healing and Axon Growth De Novo

Transplantation of adipose-derived mesenchymal stem cells (ASCs) induces tissue regeneration by accelerating the growth of blood vessels and nerve. However, mechanisms by which they accelerate the growth of nerve fibers are only partially understood. We used transplantation of ASCs with subcutaneous matrigel implants (well-known in vivo model of angiogenesis) and model of mice limb reinnervation to check the influence of ASC on nerve growth. Here we show that ASCs stimulate the regeneration of nerves in innervated mice's limbs and induce axon growth in subcutaneous matrigel implants. To investigate the mechanism of this action we analyzed different properties of these cells and showed that they express numerous genes of neurotrophins and extracellular matrix proteins required for the nerve growth and myelination. Induction of neural differentiation of ASCs enhances production of brain-derived neurotrophic factor (BDNF) as well as ability of these cells to induce nerve fiber growth. BDNF neutralizing antibodies abrogated the stimulatory effects of ASCs on the growth of nerve sprouts. These data suggest that ASCs induce nerve repair and growth via BDNF production. This stimulatory effect can be further enhanced by culturing the cells in neural differentiation medium prior to transplantation

Structure-guided selection of specificity determining positions in the human kinome

Background: The human kinome contains many important drug targets. It is well-known that inhibitors of protein kinases bind with very different selectivity profiles. This is also the case for inhibitors of many other protein families. The increased availability of protein 3D structures has provided much information on the structural variation within a given protein family. However, the relationship between structural variations and binding specificity is complex and incompletely understood. We have developed a structural bioinformatics approach which provides an analysis of key determinants of binding selectivity as a tool to enhance the rational design of drugs with a specific selectivity profile. Results: We propose a greedy algorithm that computes a subset of residue positions in a multiple sequence alignment such that structural and chemical variation in those positions helps explain known binding affinities. By providing this information, the main purpose of the algorithm is to provide experimentalists with possible insights into how the selectivity profile of certain inhibitors is achieved, which is useful for lead optimization. In addition, the algorithm can also be used to predict binding affinities for structures whose affinity for a given inhibitor is unknown. The algorithm’s performance is demonstrated using an extensive dataset for the human kinome. Conclusion: We show that the binding affinity of 38 different kinase inhibitors can be explained with consistently high precision and accuracy using the variation of at most six residue positions in the kinome binding site. We show for several inhibitors that we are able to identify residues that are known to be functionally important

Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis Risk

Human genome-wide association studies have linked single nucleotide polymorphisms (SNPs) on chromosome 9p21.3 near the INK4/ARF (CDKN2a/b) locus with susceptibility to atherosclerotic vascular disease (ASVD). Although this locus encodes three well-characterized tumor suppressors, p16INK4a, p15INK4b, and ARF, the SNPs most strongly associated with ASVD are ∼120 kb from the nearest coding gene within a long non-coding RNA (ncRNA) known as ANRIL (CDKN2BAS). While individuals homozygous for the atherosclerotic risk allele show decreased expression of ANRIL and the coding INK4/ARF transcripts, the mechanism by which such distant genetic variants influence INK4/ARF expression is unknown. Here, using rapid amplification of cDNA ends (RACE) and analysis of next-generation RNA sequencing datasets, we determined the structure and abundance of multiple ANRIL species. Each of these species was present at very low copy numbers in primary and cultured cells; however, only the expression of ANRIL isoforms containing exons proximal to the INK4/ARF locus correlated with the ASVD risk alleles. Surprisingly, RACE also identified transcripts containing non-colinear ANRIL exonic sequences, whose expression also correlated with genotype and INK4/ARF expression. These non-polyadenylated RNAs resisted RNAse R digestion and could be PCR amplified using outward-facing primers, suggesting they represent circular RNA structures that could arise from by-products of mRNA splicing. Next-generation DNA sequencing and splice prediction algorithms identified polymorphisms within the ASVD risk interval that may regulate ANRIL splicing and circular ANRIL (cANRIL) production. These results identify novel circular RNA products emanating from the ANRIL locus and suggest causal variants at 9p21.3 regulate INK4/ARF expression and ASVD risk by modulating ANRIL expression and/or structure

Detection of π+π−\pi^+\pi^-atoms with the DIRAC spectrometer at CERN

The goal of the DIRAC experiment at CERN is to measure with high precision the lifetime of the $\pi^+\pi^-$ atom ($A_{2\pi}$), which is of order $3\times10^{-15}$ s, and thus to determine the s-wave $\pi\pi$-scattering lengths difference $|a_{0}-a_{2}|$. $A_{2\pi}$ atoms are detected through the characteristic features of $\pi^+\pi^-$ pairs from the atom break-up (ionization) in the target. We report on a first high statistics atomic data sample obtained from p Ni interactions at 24 GeV/$c$ proton momentum and present the methods to separate the signal from the background.Comment: 19 pages, 12 figures, 1 tabl

Detection of Pionium with DIRAC

The aim of the DIRAC experiment at CERN is to provide an accurate determination of S-wave pion-pion scattering lengths from the measurement of the lifetime of the pi+ pi- atom. The measurement will be done with precision comparable to the level of accuracy of theoretical predictions, formulated in the context of Chiral Perturbation Theory. Therefore, the understanding of chiral symmetry breaking of QCD will be submitted to a stringent test

DIRAC Experiment and Test of Low-Energy QCD

The low-energy QCD predictions to be tested by the DIRAC experiment are revised. The experimental method, the setup characteristics and capabilities, along with first experimental results are reported. Preliminary analysis shows good detector performance: alignment error via $\Lambda$ mass measurement $m_\Lambda = 1115.6 MeV/c^2$ with $\sigma = 0.92 MeV/c^2$, $p \pi^-$ relative momentum resolution $\sigma_Q \approx 2.7 MeV/c$, and evidence for $\pi^