Chapter 16- Becoming AWARES: Mentoring Undergraduate Women in Engineering and Sciences

The Aspiration for Women’s Advancement and Retention in Engineering and Sciences (AWARES) mentorship program was designed to support women in science, technology, engineering, and mathematics (STEM) majors as they transition from their undergraduate degree programs to the workforce. The AWARES program was structured around topics relevant to women in STEM careers, including but not limited to interviewing and job offer negotiation, career development, navigating social dynamics in the workplace, and establishing and growing a professional network. Based in tenets of social cognitive theory, AWARES aims to use expert and group mentorship to increase young women’s self-efficacy for career-related soft skills that are associated with retention in STEM professions. We examined quantitative and qualitative outcomes for both mentors and mentees and found that women graduate the program with high levels of self-efficacy and that mentors also feel highly efficacious in their mentorship roles. In addition to mentee and mentor outcomes, quantitative and qualitative evaluation of the program was conducted so that participant feedback can be considered for future iterations of AWARES. Finally, program design, sustainability, and lessons learned are also discussed

Making Connections: A Handbook for Effective Formal Mentoring Programs in Academia

This book, Making Connections: A Handbook for Effective Formal Mentoring Programs in Academia, makes a unique and needed contribution to the mentoring field as it focuses solely on mentoring in academia. This handbook is a collaborative institutional effort between Utah State University’s (USU) Empowering Teaching Open Access Book Series and the Mentoring Institute at the University of New Mexico (UNM). This book is available through (a) an e-book through Pressbooks, (b) a downloadable PDF version on USU’s Open Access Book Series website), and (c) a print version available for purchase on the USU Empower Teaching Open Access page, and on Amazon

Evaluation of the Heat Inactivation of Escherichia coli and Lactobacillus plantarum by Differential Scanning Calorimetry

Differential scanning calorimetry (DSC) is used to evaluate the thermal stability and reversibility after heat treatment of transitions associated with various cellular components of Escherichia coli and Lactobacillus plantarum. The reversibility and the change in the thermal stability of individual transitions are evaluated by a second temperature scan after preheating in the DSC to various temperatures between 40 and 130°C. The viability of bacteria after a heat treatment between 55 and 70°C in the DSC is determined by both plate count and calorimetric data. The fractional viability values based on calorimetric and plate count data show a linear relationship. Viability loss and the irreversible change in DSC thermograms of pretreated whole cells are highly correlated between 55 and 70°C. Comparison of DSC scans for isolated ribosomes shows that the thermal stability of E. coli ribosomes is greater than that of L. plantarum ribosomes, consistent with the greater thermal tolerance of E. coli observed from viability loss and DSC scans of whole cells

Injury recovery of foodborne pathogens in high hydrostatic pressure treated milk during storage

Bacteria are expected to be injured or killed by high hydrostatic pressure (HHP). This depends on pressure levels, species and strain of the microorganism and subsequent storage. Injured bacteria may be repaired which could affect the microbiological quality of foodstuffs with an important safety consideration especially in low acid food products. In this Study two Gram-positive (Listeria monocytogenes CA and Staphylococcus aureus 485) and two Gram-negative (Escherichia coli O157:H7 933 and Salmonella enteritidis FDA) relatively pressure resistant strains of foodborne pathogens were pressurized at 350, 450 and 550 MPa in milk (pH 6.65) and stored at 4, 22 and 30degreesC. The results of shelf life studies indicated two types of injury, I I and 12, for all the pathogens Studied. It is obvious that 12 type injury is a major injury and after its repair (12 to I I), the cells can form colonies on non-selective but not on selective agar. The formation of colonies on both selective and non-selective agar occurs Only after full recovery Of injury (11 to AC). The results presented in this study show that even if injured cells are not detected immediately after HHP treatment, 12 type injury could be potentially present in the food system. Therefore, it is imperative that shelf life Studies must be conducted over a period of time for potential repair of 12 type injury either to detectable injury (11) or to active cells (AC) to ascertain microbiological safety of low acid food products

Evaluation of Structural Changes Induced by High Hydrostatic Pressure in Leuconostoc mesenteroides

Scanning electron microcopy (SEM), transmission electron microscopy (TEM), and differential scanning calorimetry (DSC) were used to evaluate structural changes in Leuconostoc mesenteroides cells as a function of high-hydrostatic-pressure treatment. This bacterium usually grows in chains of cells, which were increasingly dechained at elevated pressures. High-pressure treatments at 250 and 500 MPa also caused changes in the external surface and internal structure of cells. Dechaining and blister formation on the surface of cells increased with pressure, as observed in SEM micrographs. TEM studies showed that cytoplasmic components of the cells were affected by high-pressure treatment. DSC studies of whole cells showed increasing denaturation of ribosomes with pressure, in keeping with dense compacted regions in the cytoplasm of pressure-treated cells observed in TEM micrographs. Apparent reduction of intact ribosomes observed in DSC thermograms was related to the reduction in number of viable cells. The results indicate that inactivation of L. mesenteroides cells is mainly due to ribosomal denaturation observed as a reduction of the corresponding peak in DSC thermograms and condensed interior regions of cytoplasm in TEM micrographs

Optimization of growth for the hyperthermophilic archaeon Aeropyrum pernix on a small-batch

Abstract: Growth of Aeropyrum pernix, the first reported aerobic neutrophilic hyperthermophilic archaeon, was investigated under different cultivation parameters. Different sources of seawater, pH, and the cultivation methods were tested with the aim to improve the biomass production. A 1-L glass flask fitted with a condenser and air diffuser was used as a bioreactor. The optimum conditions for maximizing A. pernix biomass were obtained when Na 2 S 2 O 3 ·5H 2 O (1 g/L) with added marine broth 2216 at pH 7.0 (20 mmol HEPES buffer/L) was used as a growing medium in a 1-L flask. The biomass production was 0.45 g dry cell mass/L in 40 h under the optimum conditions, which is more than the 0.42 g dry cell mass/L in 60 h previously obtained. Key words: Aeropyrum pernix, hyperthermophilic archaeon, biomass production, simple glass flask based system. Mots clés : Aeropyrum pernix, archéen hyperthermophile, production de biomasse, système simple reposant sur un flacon de verre