ZINC IN SEPARATED SEQUENTIAL FRACTIONS FROM SIX SOILS ON THE SLOPES OF THE SIEDLCE HIGH PLAIN

The aim of the study was to evaluate the total content of zinc in the fractions separated from the soils located in two transects on the moraine slope of the Siedlecka High Plain (some situated above and some beneath the underground municipal landfill site). The highest total content of zinc was detected in the soils situated beneath the underground municipal landfill site. The sequential fractioning was performed by the Zeien-Brümmer method. The speciation analysis revealed that this metal was bound to different components of the soil solid phase. The highest percentage of zinc content was detected in the residual fraction F7 in the parent rock horizons and the lowest was noted in the bioavailability fractions, i.e. exchangeable F2 and easilyeasily soluble F l. In transects A and B of the tested soils significant correlations were found between the content of zinc in the separated fractions and its total content, the total content of iron and manganese, clay fraction <0.002 mm, cation exchange capacity (CEC) as well as between the content of carbon organic compound and zinc in the F4 organic fraction

Seasonal dynamics of dry matter accumulation and nutrients in a mature miscanthus × giganteus stand in the lower silesia region of poland

Funding: J.C.B. and P.R.H.R. were funded by the Biotechnology and Biological Sciences Research Council strategic programme grant on Resilient Crops (BBS/E/W/10963A01).Peer reviewedPublisher PD

INFLUENCE OF LIMING AND WASTE ORGANIC MATERIALS ON THE ACTIVITY OF PHOSPHATASE IN SOIL CONTAMINATED WITH NICKEL

A study was carried out on soil following a two-year pot experiment that was conducted in 2009–2010, in three repetitions in Siedlce. The experiment included the following factors: 1 – amount of Ni in soil (0, 75, 150 and 225 mg·kg-1 soil by applying an aqueous NiSO4·7H2O solution); 2 – liming (0 and Ca according to 1 Hh as CaCO3); 3 – organic waste products (rye straw at a dose of 4 t·ha-1 and brown coal at a dose of 40 t·ha-1). In each experimental year, orchard grass was the test plant and four swaths were harvested. The activities of acidic and alkaline phosphatase, pH and the content of carbon in organic compounds were determined in the soil samples collected after each grass swath and in each experimental year. It was found that Ni at 75 mg·kg-1 soil activated the enzymes under study, whereas higher doses caused their statistically-confirmed inactivation. The lowest activity of the investigated enzymes was detected in soil supplemented with 225 Ni·kg-1 soil. Liming caused an increase in the activity of alkaline phosphatase and a reduction in the activity of acidic phosphatase. Straw and brown coal induced a substantial increase in the activity of both enzymes in the tested soil samples. Both liming and straw and carbon eliminated the negative effect of higher nickel doses on the activity of the enzymes under study

INFLUENCE OF LIMING AND WASTE ORGANIC MATERIALS ON THE ACTIVITY OF UREASE AND DEHYDROGENASE IN SOIL CONTAMINATED WITH NICKEL

In soil sampled after a two-year pot experiment, the activity of urease and dehydrogenase and content of nitrogen have been determined. The experiment included three factors: 1. the amount of nickel added to the soil (0, 75, 150 and 225 mg · kg-1 of soil), 2. liming (0 and Ca according to 1 Hh hydrolytic acidity), 3. organic materials (straw of rye and brown coal). Test plant was cocksfoot, which four cuts were collected in each growing season. It was found that nickel added to the soli in dose of 75 mg · kg-1 activates enzymes studied, whereas higher doses cause them explicit deactivation. Both liming and waste organic materials limited the negative effect of higher doses of nickel on the activity of dehydrogenase and urease. Simultaneously, both straw and brown coal caused a slight increase in the amount of nitrogen in the soil

Effect of liming and use of organic materials on the contents of cooper adn zinic in of cock`s-foot grown in soil contaminated with nickel

W doświadczeniu wazonowym badano wpływ wapnowania i stosowania materiałów organicznych – słomy i węgla brunatnego na zawartość miedzi oraz cynku w kupkówce pospolitej (Dactylis glomerata L.) uprawianej na glebie w różnym stopniu zanieczyszczonej niklem. Analizowano rośliny czterech pokosów trawy zebrane w trzecim roku doświadczenia. Wapnowanie powodowało istotne zmniejszenie zawartości miedzi w roślinie testowej, nie różnicując jednoznacznie zawartości cynku. W przeprowadzonych badaniach nie wykazano istotnego wpływu zróżnicowanej ilości niklu w glebie na zawartość cynku w kupkówce pospolitej, natomiast wraz ze wzrostem stopnia zanieczyszczenia gleby niklem, zawartość miedzi w trawie uległa zmniejszeniu, co świadczyć może o antagonizmie obu mikroelementów.The effect of liming and an addition of waste organic materials to soil on the contents of copper and zinc in cock`s-foot (Dactylis glomerata L.) cultivated on soil contaminated to various degrees with nickel was studied in a pot culture experiment. Plants from four cuts of grass were analysed. Liming resulted in a significant reduction of copper content in the plant test, without any distinct differentiation in zinc content. The studies showed no effect of varying amounts of nickel in the soil on zinc content in the orchard grass, and with increasing soil contamination with nickel the content of cooper in the grass decreased, which may be an evidence of antagonism of the two micronutrients

THE DISTRIBUTION OF ZINC, NICKEL AND CHROMIUM IN BIOMASS OF FERTILIZED Salix viminalis L.

The distribution of zinc, nickel and chromium in the leaves and branches of three varieties of Salix viminalis differentiated fertilized was investigated. The biomass of Salix viminalis was harvested for investigation in the first and second year of application of mineral fertilizers and waste activate sludge in doses which contained 50, 150 and 200 kg of total nitrogen. The content of investigated metals was significantly differentiated upon the part of plant and fertilization. The amount of zinc, nickel and chromium were higher in leaves than in branches. The biomass of Salix viminalis contained the highest amount of zinc and the lowest of chromium. The highest content of zinc was determined in the biomass of Salix purpurea but lowest nickel and chromium in S. alba. The fertilization had differentiated influence upon the content of determined elements

A New Method for Sequential Fractionation of Nitrogen in Drained Organic (Peat) Soils

The aim of this study was to assess the transformation of organic matter in organic soils undergoing a phase of secondary transformation, based on a new method of nitrogen compound fractionation. Laboratory tests were carried out for 31 layers of muck (after secondary transformation) and peat (parent material of the soil) of drained organic soils (peat). The new method consists of sequential extraction in the following steps: (1) 0.5 M K2SO4 (extraction at room temperature); (2) 0.25 M H2SO4 (hot hydrolysis) (3) 3.0 M H2SO4 (hot hydrolysis); and (4) concentrated H2SO4 (mineralization of the post-extraction residue). As a result of the extraction process, the following fractions (operating forms) were obtained: mineral nitrogen (Nmin), dissolved organic nitrogen (N-DON), readily hydrolyzing organic nitrogen (N-RH), non-readily hydrolyzing organic nitrogen (N-NRH), and non-hydrolyzing organic nitrogen (N-NH). The study demonstrates the usefulness of the applied method for assessing the degree of secondary transformation of drained organic soils. The obtained results of nitrogen fractionation indicate the significant dynamics of nitrogen forms’ transformations and a significant relationship between these forms and soil properties. Nitrogen transformation processes during the secondary transformation process after dehydration resulted in an increase in the share of N-DON (on average: 1.47% of Norg for the peat layers and 2.97% of Norg for the muck layers) and in an increase in the share of NRHON (on average: 20.7% of Norg for the peat layers and 33.5% of Norg for the muck layers). The method of sequential nitrogen fractionation used in our study allowed us to define an index determining the degree of transformation of organic matter in peat after drying. We defined it as the ratio of readily hydrolyzable forms (the fraction is very variable in the secondary transformation process) to non-readily hydrolyzable forms (relatively stable fraction in the secondary transformation process): N-RH/N-NRH. The average value of this index was significantly lower in the peat layers (0.64 on average) than in the muck beds (1.04 on average). The value of this index is significantly correlated with soil properties: bulk density (R2 = 0.470); general porosity (R2 = 0.503); total carbon content (TC) (R2 = 0.425); total carbon to total nitrogen ratio (TC/TN) (R2 = 0.619); and share of carbon of humic substances (C-HS) (R2 = 0.466). We believe that the method of sequential nitrogen fractionation may be useful for other soils and organic materials

The Nitrogen Fixation and Yielding of Pea in Different Soil Tillage Systems

The field experiment was carried out in 2013 and 2014 as part of a long-term experiment to test the influence of different soil tillage systems (conventional, reduced, direct sowing) on (a) the yield of summer pea (Pisum sativum L.) and (b) the amount of biologically reduced atmospheric nitrogen (N), which was determined using the 15N dilution method (ID15N). Spring barley was used as a reference plant. Climatic conditions did not have a significant influence on the yield of pea seeds (mean value 4.56 t ha−1), post-harvest residues (3.76 t ha−1) and total biomass (8.33 t ha−1). Soil tillage system was found to have a significant impact on all components of the pea yield in years of experiment, with the highest average seed yield values observed with the conventional system (5.19 t ha−1) and significantly lower values observed with reduced tillage (4.34 t ha−1) and direct sowing (4.17 t ha−1). The content of total N was greatest in the pea biomass that was harvested in the conventional system (202 kg ha−1) and lowest with direct sowing (155 kg ha−1). Nitrogen accumulated in the pea seeds from three sources: atmosphere (mean value 35.2%), fertilizers (6.8%) and soil (57.9%), equating to 48.6, 9.9, and 85.4 kg ha−1, respectively. Soil tillage system was found to have a significant impact on the amount of N that was fixed from the atmosphere by the peas: 17.7% in conventional tillage, 37.9 in reduced system and 50.2% in direct sowing, which equates to a harvested seed yield of 28.9, 52.0 and 64.4 kg ha−1, respectively. In the post-harvest residues, the amount of N fixed from the atmosphere by the pea crop was also modified by the soil tillage system in range: 20.2% in conventional tillage and 32.6% in direct sowing (which equates to 7.4 and 8.5 kg N ha−1, respectively), but the difference was not significant

The Nitrogen Fixation and Yielding of Pea in Different Soil Tillage Systems

The field experiment was carried out in 2013 and 2014 as part of a long-term experiment to test the influence of different soil tillage systems (conventional, reduced, direct sowing) on (a) the yield of summer pea (Pisum sativum L.) and (b) the amount of biologically reduced atmospheric nitrogen (N), which was determined using the 15N dilution method (ID15N). Spring barley was used as a reference plant. Climatic conditions did not have a significant influence on the yield of pea seeds (mean value 4.56 t ha−1), post-harvest residues (3.76 t ha−1) and total biomass (8.33 t ha−1). Soil tillage system was found to have a significant impact on all components of the pea yield in years of experiment, with the highest average seed yield values observed with the conventional system (5.19 t ha−1) and significantly lower values observed with reduced tillage (4.34 t ha−1) and direct sowing (4.17 t ha−1). The content of total N was greatest in the pea biomass that was harvested in the conventional system (202 kg ha−1) and lowest with direct sowing (155 kg ha−1). Nitrogen accumulated in the pea seeds from three sources: atmosphere (mean value 35.2%), fertilizers (6.8%) and soil (57.9%), equating to 48.6, 9.9, and 85.4 kg ha−1, respectively. Soil tillage system was found to have a significant impact on the amount of N that was fixed from the atmosphere by the peas: 17.7% in conventional tillage, 37.9 in reduced system and 50.2% in direct sowing, which equates to a harvested seed yield of 28.9, 52.0 and 64.4 kg ha−1, respectively. In the post-harvest residues, the amount of N fixed from the atmosphere by the pea crop was also modified by the soil tillage system in range: 20.2% in conventional tillage and 32.6% in direct sowing (which equates to 7.4 and 8.5 kg N ha−1, respectively), but the difference was not significant