Additional file 1: Table S1. of Post-transcriptional regulation of SHANK3 expression by microRNAs related to multiple neuropsychiatric disorders

The putative miRNA families targeting the SHANK3 3′UTR predicted by TargetScan (release 6.2). The number of binding sites for each miRNA family, and the binding type (8mer, 7mer-m8, or 7mer-1A) are described. Table S2. Altered expression profiles of miR-7, miR-34a, and miR-504 in multiple neuropsychiatric disorders. Table S3. Summary of statistical analyses for the experiments. Figure S1. The secondary structures of SHANK3 (a) and SHANK2 (b) 3′UTRs predicted by RNAfold ( http://rna.tbi.univie.ac.at/cgi-bin/RNAfold.cgi ) according to the minimum free energy. The color code represents base-pair probabilities. Figure S2. The expression of Shank3 construct with 3′UTR, but not that without 3′UTR, was significantly decreased by miR-7 and miR-504 in mouse cultured neurons. Statistical analyses are in Additional file 1: Table S3. Figure S3. The ten major Shank3 isoforms (Wang et al. Molecular Autism 2014, 5:30) (a), and the nucleotide sequence of the last exon (exon 22) of Shank3 gene (b). The Shank3 isoforms containing SAM domain at the C-terminus are labeled with red color. (DOCX 3382 kb