Effects of evodiamine(EVO)on the proliferation of MCF-7 and MDA-MB-231.

<p>The incubation period was from 1 to 4 days. Proliferation index was measured by MTT assay. Each value presents mean plus or minus SEM. * <i>p</i><0.05, ** <i>p</i><0.01 as compared to corresponding vehicle group.</p

Effects of evodiamine(EVO)on the mRNA (a.) and protein (b.) expression of Nbk (Bik) in MCF-7 cells which were treated for 18 hrs.

<p>The media for MCF-7 were phenol red-free DMEM/F12 supplemented with 2% charcoal/dextran-stripped FBS and estradiol(10⁻⁹ M). Cell lysates were analyzed by RT-PCR and Western blot. Each value presents mean plus or minus SEM. ** <i>p</i><0.01 compared to vehicle group.</p

Effects of ICI-182,780(ICI), evodiamine(EVO)and ICI combined with EVO on the proliferation of MCF-7 cells.

<p>The incubation period was from 1 to 4 days. Proliferation index was measured by MTT assay. Each value presents mean plus or minus SEM. ++<i>p<</i>0.01 as compared to vehicle of day 1, *<i>p<</i>0.05 and **<i>p<</i>0.01 as compared to corresponding vehicle group. #<i>p<</i>0.05 as compared to ICI treated group.</p

Effects of evodiamine (EVO) on the protein expression of PARP and cleaved-PARP in MCF-7 cells treated with evodiamine for 24 or 48 hr.

<p>Cell lysates were analyzed by Western blot. Each value presents mean plus or minus SEM. ** <i>p</i><0.01 as compared to corresponding vehicle group.</p

Morphological change of MCF-7 and MDA-MB-231 cells after administration with evodiamine (1×10 ⁻⁶, 1×10⁻⁵ M) for 24 or 48 hrs.

<p>Morphological change of MCF-7 and MDA-MB-231 cells after administration with evodiamine (1×10 ⁻⁶, 1×10⁻⁵ M) for 24 or 48 hrs.</p

Effects of evodiamine(EVO)on the protein expression of Bax in MCF-7 cells which were treated for 18 hrs.

<p>The media for MCF-7 cells were phenol red-free DMEM/F12 supplemented with 2% charcoal/dextran-stripped FBS and estradiol(10⁻⁹ M). Cell lysates were analyzed by Western blot. Each value presents mean plus or minus SEM. ** <i>p</i><0.01 as compared to vehicle group.</p

Effects of evodiamine (EVO) on the protein expression of ERα (a.), ERβ (b.) and mRNA of ERα (c.) in MCF-7 cells which were treated with evodiamine for 24 or 48 hr for the detection of protein, and 18-hrs culture was for mRNA measurement.

<p>Cell lysates were analyzed by Western blot (<b>a. and b.</b>) or RT-PCR (<b>c.</b>). Each value presents mean plus or minus SEM. **<i>p</i><0.01 as compared to corresponding vehicle group.</p

Effects of evodiamine(EVO)on the protein expression of procaspase 7 and cleaved-caspase 7 in MCF-7 cells treated with evodiamine for 24 or 48 hr.

<p>Cell lysates were analyzed by Western blot. Each value presents mean plus or minus SEM. ** <i>p</i><0.01 as compared to corresponding vehicle group.</p

Basal levels of rat plasma glucose, lactate, sodium and potassium in different experiments.

a<p>Mean±SEM.</p><p>Basal levels of rat plasma glucose, lactate, sodium and potassium in different experiments.</p

Effects of sodium lactate (1–10 mM) on the vehicle or angiotensin II (10⁻⁸ M)-stimulated aldosterone release by rat ZG cells.

<p>The challenge medium of glucose concentration was 200 mg/dl. +<i>P</i><0.05 <i>vs.</i> basal level by the Student's paired <i>t</i>-test; *<i>P</i><0.05 <i>vs.</i> lactate = 1 mM by the two way ANOVA.</p