Specificity of TaqMan miRNA assays between stem–loop and linear RT primers

<p><b>Copyright information:</b></p><p>Taken from "Real-time quantification of microRNAs by stem–loop RT–PCR"</p><p>Nucleic Acids Research 2005;33(20):e179-e179.</p><p>Published online 27 Nov 2005</p><p>PMCID:PMC1292995.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Mature let-7a-specific assay was tested against let-7a, let-7e and pri-miR precursor let-7a-3. Δ represents the difference between two targets or methods. A total of 1.5 × 10 copies of synthetic targets were added to each RT reaction