Effects of local simvastatin on periosteal distractiono steogenesis in rabbits

PubMedID: 25640700Our aim was to evaluate the effect of local simvastatin on the formation of new bone using a new design of periosteal distractor. The distractorswere placed between the periosteum and bone at the inferior border of the mandible of 20 New Zealand rabbits. In the first group (n = 10)simvastatin was applied locally to the distraction zone. The other 10 rabbits served as controls. The formation of new bone was evaluated withdigital direct radiography, computed tomography (CT), and histomorphometric analyses. New bone formed in all rabbits, but more formedin the experimental group according to histomorphometric variables. However, other measurements did not differ significantly between thegroups. The new design of the periosteal distraction device was successful in causing new bone to form. Local simvastatin made no significantcontribution to the procedure. © 2015 The British Association of Oral and Maxillofacial Surgeons.Firat University Scientific Research Projects Management Unit: DHF2012D08The study was approved by the Ethics Review Committee of Cukurova University, conducted in accordance with the guidelines of the Cukurova University Animal Research Centre, and supported by Cukurova University Scientific Research Projects Grant, Adana, Turkey ( DHF2012D08 )

Comparative study of the osseous healing process following three different techniques of bone augmentation in the mandible: An experimental study

PubMedID: 25091894The aim of this study was to evaluate the osseointegration of three different bone grafting techniques. Forty-eight mature New Zealand rabbits were divided randomly into three groups of 16 each. Horizontal augmentation was performed on the corpus of the mandible using three different techniques: free bone graft (FBG), free periosteal bone graft (PBG), pedicled bone flap (BF). The animals were sacrificed at postoperative weeks 1, 3, or 8. Specimens were decalcified for histological examination, and histomorphometric measurements were performed. The histological evaluation demonstrated bony fusion between the grafts and the augmented mandibular bone after 8 weeks in all groups. At week 8, the bone volume was significantly greater in the BF group than in the FBG (P < 0.001) and PBG (P = 0.001) groups, and also the trabecular thickness was significantly greater than in the FBG (P = 0.015) and PBG (P = 0.015) groups. Trabecular separation was significantly lower in the BF group than in the FBG group at week 8 (P = 0.015). BF demonstrated greater osseous healing capacity compared to FBG and PBG. The preserved vascularization in BF improves the bone quality in mandibular bone augmentations. © 2014 International Association of Oral and Maxillofacial Surgeons

Vertical bone augmentation using bone marrow-derived stem cells: An in vivo study in the rabbit calvaria

PubMedID: 26397478Purpose: To evaluate the bone regeneration capacity of bone marrow-derived stem cells (BMSCs) in vertical guided augmentation of bone tissue. Material and Methods: The calvaria of 20 rabbits were vertically augmented with autogenous bone graft (ABG); collagen/beta-tricalcium phosphate (ß-TCP) linked scaffold transplanted with 15 × 10 4 BMSCs; or scaffold alone (control). The augmentation materials were covered with stainless steel domes. BMSCs were isolated with Ficoll-Paque technique and applied directly without in vitro expansion. The newly formed bone was evaluated using radiodensitometric, histomorphometric, histological, and micro-computed tomographic (micro-CT) analyses after a 12-week healing period. The data excluding micro-CT assessments were compared statistically. Results: Radiodensitometric and bone volume parameters demonstrated increased bone formation in both BMSC group and ABG group compared with control group (P 0.05). The mean histological scores for the BMSC, ABG, and control groups were 7.44 ± 1.03, 8.44 ± 0.81, and 6.00 ± 1.10, respectively, indicating significant difference among the groups (P < 0.05). Conclusion: BMSCs delivered with a collagen/ß-TCP linked scaffold can provide improved new bone formation that is comparable with autogenous bone block graft through vertical guided bone regeneration technique. © 2015 Wolters Kluwer Health, Inc