Probiotic supplementation effects on milk fatty acid profile in ewes

We hypothesised that probiotic feeding would alter the fatty acid (FA) profile of sheep's milk. Sixteen lactating ewes, kept under the same feeding and management practices, were randomly allocated to receive either a control diet or the same diet supplemented with a commercial multi-strain bacterial probiotic. Milk fat FA contents were monitored fortnightly for eight consecutive weeks from 14 d after lambing. Probiotic supplementation increased the contents of butyric and caproic acids in milk fat and had no negative effects on other relevant FA from the human's health point of view (i.e., no differences in branched chain, vaccenic, rumenic and n-3 FA were observed). Under the conditions assayed in the present work, the contents of milk FA originated from rumen microbial metabolism were scantly altered, which suggests that the rumen conversion pathways of FA were not substantially modified by the probiotics.Peer reviewe

Detection of Brucella by Peripheral Blood PCR and Comparison with Culture and Serological Methods in Suspected Cases

"nBackground: Brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its tradi­tional diagnosis is based on blood culture and serological methods. For more sensitive and specific detection, the PCR method is recommended."nMethods: One hundred four blood samples were gathered from suspicious patients with brucellosis different ages form Ker­manshah, Mazandaran, Khorassan and Hormozgan provinces, and were examined by Rose Bengal, anti globulin, culture and PCR methods."nResults: Seventy three samples were positive by PCR method, 15 samples were positive by cultured method and 84 sam­ples were positive by serological methods."nConclusion:  PCR method is sensitive and specific for diagnosis of Brucella from peripheral blood in suspected cases. &nbsp