Effect of rbST on Serum Biochemical Values During Various Physiological and Weather Conditions in Kundhi Buffaloes

This study was performed to investigate the effect of rbST treatment on some biochemical parameters in addition to values of thyroid hormones in Kundhi buffaloes. Sixteen Kundhi buffaloes were divided into two groups. Group-A (N=8) served as control and Group-B (N=8) was treated with 250 mg of rbST fortnightly for 1st lactation. Blood samples were collected weekly during lactation period from day one to 60 as pre-treatment values and then from day 61 to completion of 1st lactation in Kundhi buffaloes as post-treatment values. The findings revealed overall non significant variation in the serum biochemical and thyroid hormone values between control and rbST treated buffaloes, whereas, significant differences were observed during various physiological and weather conditions in both groups. Glucose and uric acid values were observed higher during parturition and winter season in Kundhi buffaloes. Protein level was higher in rbST treated buffaloes during open days

Ultrasonographic Biometrical Studies on Reproductive Organs of rbST Treated Pregnant Kundhi Buffaloes

Biometrical studies were conducted on sixty four gravid uteri with estimated gestation period of 1st, 2nd, 3rd and 4th month of pregnancy in Kundhi buffaloes. Sixteen specimens for each month of gestation were collected from Hyderabad slaughterhouse to evaluate techniques for measurement of reproductive organs. The measurements for fetometry and morphometry of reproductive organs by ultrasound machine and manually (by measuring tape and vernier caliper) were performed and compared. The Ultrasound examinations were performed through diagnostic ultrasound machine equipped with multi-frequency 5-10MHz transrectal linear array transducer. Analysis revealed that the ovary, foetus, foetal fluid and uterus progressively increased significantly (P<0.01) in weight during 4th month of the gestation than 1st, 2nd and 3rd month of gestation period in rbST treated Kundhi buffaloes. Ovaries with CL were significantly (P<0.01) heavier than the ovary in which CL was absent. Corpus leutum was significantly lighter (P<0.01) during 1st month of pregnancy than 2nd, 3rd and 4th month of pregnancy in rbST treated Kundhi buffaloes. Gravid uterus was significantly heavier (P<0.01) during 4th month of pregnancy than 1st, 2nd and 3rd month of pregnancy in rbST treated Kundhi buffaloes. There was no significant difference between the measurements of oviduct, cervix and vagina during 1st to 4th month of pregnancy. The placenta, foetal fluid and foetus progressively increased significantly in size as gestation period progressed. The ultrasonographic fetometric and morphometric threshold of CRL, TD and UD and foetal weight was steadily increased in rbST treated Kundhi buffaloes. There was significant increase (P<0.05) in the CRL, TD, UD and foetus weight/volume at 4th month of pregnancy than 1st , 2nd and 3rd month of pregnancy in rbST treated Kundhi buffaloes. In conclusion, the overall data indicated that the examination for ultrasonographic fetometry and morphometry of reproductive organs of kundhi buffaloes for evaluation of fetal development, estimation of gestational age and formulation of fetal chart through reproductive organs were more suitable as compare to manual biometrical evaluation

IMPACT OF THEILERIOSIS ON MILK IN NATURALLY INFECTED COWS AND BUFFALOES AT HYDERABAD

Study was conducted to investigate the consequences of Theileriosis in naturally infected cattle and buffaloes of urban and peri urban areas of Hyderabad. Chemical analysis of milk of infected cows and buffaloes revealed that protein, ash and lactose were significantly declined (P<0.05) whereas, fat and total solids were significantly increased (P<0.05) in Theileria infected cows and buffaloes, but pH and acidity was unaffected. Financial losses occur in terms of reduced quality and severely decreased quantity of milk and meat. The total financial loss including the mortality and decreased quantity of milk and meat was recorded of Rs. 0.17 million / animal in the current study. Theileriosis is one of the main causes of financial losses for livestock holders

HISTOPATHOLOGICAL EVALUATION OF TWO SUTURING TECHNIQUES FOR END-TO-END ANASTOMOSIS OF COLON OF DOGS

An experimental study was conducted on 12 healthy adult dogs to determine the best method for anastomosis of colon. An appositional 2 layer (APP-2) suture pattern was compared with the inverting 2 layer (INV-2) suture pattern for end-to-end anastomosis. VicrylR (size 3-0) was used for anastomosis. At day 10 after surgery, all anastomotic lines were apposed but the mucosa did not cover the lumen of the anastomosis with APP-2 layer suture pattern. The specimens closed w1th INV-2 layer suture pattern showed more pronounced inversion of all layers and the cut ends of the mucosa and submucosa at the tip of told were apposed by a thick plug of fibrous connective tissue. At day 20, the mucosa covered the internal surface and bridged the narrow detect with a single layer of epithelial cells with APP-2 layer suture pattern. The specimens closed With INV-2 layer suture pattern showed slight inversion of the serosa and smooth. muscle caused moderate thickening of the wall. Histopathogical differences between samples collected at 30 days were associated mostly with the maturation process. The current study showed the superiority of the APP-2 layer suture pattern for colonic end-to-end anastomosis in terms of better apposition of the anastomotic layers and better anastomotic healing over the INV -2 layer suture pattern