Organ Related Markers and Functional Test [Mean (SD); (n = 4 or 8)].

<p>Organ Related Markers and Functional Test [Mean (SD); (n = 4 or 8)].</p

Hematologic Parameters Post Gastrointestinal Radiation Injury [Mean (SD); (n = 4)].

<p>Hematologic Parameters Post Gastrointestinal Radiation Injury [Mean (SD); (n = 4)].</p

Intestinal Cytokine levels (pg/mg protein) Post Gastrointestinal Radiation Injury [Mean (SD); (n = 4)].

<p>Intestinal Cytokine levels (pg/mg protein) Post Gastrointestinal Radiation Injury [Mean (SD); (n = 4)].</p

Representative Images (H&E) of Jejunum Sections at 15.7 Gy PBI in Male Mice at Day 10.

<p>A: Non-irradiated control from day 4; G: PF4; H: PF7; I: PF4 + PF7; J: Amifostine; K: Irradiated control. Pictures are 600 μm x 600 μm.</p

Regression line shows LD50/30 is 15.7 Gy.

<p>Shown is the % Mortality as a function of the partial body irradiation (PBI) dose received. Male C57BL/6J mice (9–10 weeks old; 14 animals per group) were irradiated at a dose range of 14.5–16.5 Gy using a 6MV LINAC photon source (Varian model #EX-21) while the head, forelimbs, and thorax were shielded. Following irradiation the animals were observed for 30 days. Mortality was seen in the range of 7–90% on days 6–8 after PBI. Probit analysis was done and percent mortality was plotted against radiation dose received (R² is 0.947 indicating a very good fit). The Fisher’s exact test for data points indicate there are statistically significant differences (p<0.05) between 14.5 vs. 16.0 Gy, 14.5 vs. 16.5 Gy, 15.0 vs. 16.5 Gy, and 15.5 vs. 16.5 Gy. From these data the LD30/30, LD50/30, and LD70/30 were established at the levels of 15.3 Gy at a 95% CI of 14.912–15.687 Gy, 15.7 Gy at a 95% CI of 15.307–16.082 Gy, and 16.1 Gy at a 95% CI of 15.702–16.477 Gy, respectively. With R-squared, Fisher’s exact test, and 95% CI of LD30/30, LD50/30, and LD70/30, a high correlation is demonstrated between dose and mortality.</p

Villus length and Crypt number Post Gastrointestinal Radiation Injury [Mean (SD); (n = 8)].

<p>Villus length and Crypt number Post Gastrointestinal Radiation Injury [Mean (SD); (n = 8)].</p

Summary of Changes in Blood and Tissue Parameters With and Without Mitigation After PBI Injury.

<p>Summary of Changes in Blood and Tissue Parameters With and Without Mitigation After PBI Injury.</p

Kaplan-Meier Survival Curves of C57BL/6J Mice (n = 30/group), GI irradiated with 15.7 Gy (LD50/30).

<p>Twenty four hours after irradiation and daily for 7 days, mice were treated as indicated with 3 mg/kg unformulated FGF4, 3 mg/kg unformulated FGF7, 3 mg/kg PF4, 3 mg/kg PF7, PF4/7 which is 1.5 mg/kg PF4 plus 1.5 mg/kg PF7, or 60 mg/kg PGC carrier. Amifostine (300 mg/kg) used as a positive control was administered once 30 min prior to irradiation, and the sham irradiated control group remained untreated. Compared to the sham irradiated control (60% survival), significant survival advantage (≥90% survival) was provided by PF7, PF4, or unformulated FGF7 [p<0.02 by both Log-rank (Mantel-Cox) and Chi-square tests]. PF4, PF7, and Amifostine are not significantly different in preventing animal death. The PF4 and PF7 treated groups have higher survival than unformulated FGF4 or FGF7.</p