High-resolution nested model for the Lebanese coastal area, Eastern Mediterranean: implementation and climatological runs

International audienceAs a part of the project Mediterranean Network to Assess and Upgrade Monitoring and Forecasting Activity in the Region (MAMA) we implemented a high resolution nested hydrodynamic model (1/40° horizontal grid, 16 sigma levels) for the coastal, shelf and open sea areas off the Lebanese coast, East Levantine Basin of the Eastern Mediterranean Sea. The Lebanese Shelf Model (LSM) is a version of the Princeton Ocean Model (POM). It is nested in a coarse resolution model the Aegean Levantine Eddy Resolving Model (1/20° horizontal grid, 25 sigma levels), ALERMO, that covers the Eastern Mediterranean. The nesting is one way so that velocity, temperature, and salinity along the open boundaries are interpolated from the relevant coarse model variables. Numerical simulations have been carried out under climatological surface and lateral forcing. Due to the relatively small domain, the results closely follow the simulation of the intermediate model with more details especially over the narrow shelf region. Simulations reproduce main circulation features and coastal circulation characteristics over the eastern Levantine shelf. This paper describes the modeling system setup, compares the simulations with the corresponding results of the coarse model ALERMO, and with the observed climatological circulation characteristics in the Levantine Basin off the Lebanese coast

Complications and pitfalls of lumbar interlaminar and transforaminal epidural injections

Lumbar interlaminar and transforaminal epidural injections are used in the treatment of lumbar radicular pain and other lumbar spinal pain syndromes. Complications from these procedures arise from needle placement and the administration of medication. Potential risks include infection, hematoma, intravascular injection of medication, direct nerve trauma, subdural injection of medication, air embolism, disc entry, urinary retention, radiation exposure, and hypersensitivity reactions. The objective of this article is to review the complications of lumbar interlaminar and transforaminal epidural injections and discuss the potential pitfalls related to these procedures. We performed a comprehensive literature review through a Medline search for relevant case reports, clinical trials, and review articles. Complications from lumbar epidural injections are extremely rare. Most if not all complications can be avoided by careful technique with accurate needle placement, sterile precautions, and a thorough understanding of the relevant anatomy and contrast patterns on fluoroscopic imaging

Loss of C-5 Sterol Desaturase Activity Results in Increased Resistance to Azole and Echinocandin Antifungals in a Clinical Isolate of Candida parapsilosis

Among emerging non-albicans Candida species, Candida parapsilosis is of particular concern as a cause of nosocomial bloodstream infections in neonatal and intensive care unit patients. While fluconazole and echinocandins are considered effective treatments for such infections, recent reports of fluconazole and echinocandin resistance in C. parapsilosis indicate a growing problem. The present study describes a novel mechanism of antifungal resistance in this organism affecting susceptibility to azole and echinocandin antifungals in a clinical isolate obtained from a patient with prosthetic valve endocarditis. Transcriptome analysis indicated differential expression of several genes in the resistant isolate, including upregulation of ergosterol biosynthesis pathway genes ERG2, ERG5, ERG6, ERG11, ERG24, ERG25, and UPC2. Whole-genome sequencing revealed that the resistant isolate possessed an ERG3 mutation resulting in a G111R amino acid substitution. Sterol profiles indicated a reduction in sterol desaturase activity as a result of this mutation. Replacement of both mutant alleles in the resistant isolate with the susceptible isolate's allele restored wild-type susceptibility to all azoles and echinocandins tested. Disruption of ERG3 in the susceptible and resistant isolates resulted in a loss of sterol desaturase activity, high-level azole resistance, and an echinocandin-intermediate to -resistant phenotype. While disruption of ERG3 in C. albicans resulted in azole resistance, echinocandin MICs, while elevated, remained within the susceptible range. This work demonstrates that the G111R substitution in Erg3 is wholly responsible for the altered azole and echinocandin susceptibilities observed in this C. parapsilosis isolate and is the first report of an ERG3 mutation influencing susceptibility to the echinocandins

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

SOURCES OF PENETRATION AND PCD-MEDIATED RESISTANCE TO GRAPEVINE POWDERY MILDEW IN THE VITACEAE FAMILY

Erysiphe necator is the causal agent of powdery mildew in the cultivated grapevine (Vitis vinifera). V. vinifera is a member of the Vitaceae family which contains over 700 species in 14 genera. Although V. vinifera is highly susceptible to Erysiphe necator, other species within the Vitaceae family have been reported to show powdery mildew resistance, but little is known about the mechanistic basis of this resistance. Therefore, the frequency of E. necator penetration events, in addition to programmed cell death (PCD) responses, were investigated in a representative genotype from a range of different members of the Vitaceae family. The results reveal that penetration resistance and PCD-mediated responses or combinations of both are employed by members of the Vitaceae genera to limit E. necator infection. Potential, new sources of resistance were also identified.</p