Endothelial Nitric Oxide Synthase and Angiotensin Converting Enzyme Gene Polymorphisms in MigrainePatients

Giriş: Bu çalışmada migren ile endotelyal nitrik oksit sentaz (eNOS) geni intron 4de, 27 bazlık tekrarlardan oluşan Ardışık Kopya Sayısı Tekrarları (VNTR) ve anjiyotensin dönüştürücü enzim (ADE) genindeki insersiyon/delesyon polimorfizmlerinin ilişkisi araştırıldı. Yön­tem­ler: Çalışmaya 105 migren başağrısı olan ve 97 sağlıklı kadın birey alındı. Migren hastaları auralı ve aurasız olmak üzere iki gruba ayrılırken, migren atak sıklığı ve şiddeti kaydedildi. eNOS VNTR (eNOS 4a/b) ve ADE insersiyon/delesyon polimorfizmleri (ADE I/D) polimorfizmleri polimeraz zincir reaksiyonu yöntemi ile belirlendi. Bul­gu­lar: eNOS 4 a/b gen polimorfizminin alel ve genotip sıklıkları migren ile kontrol grubu arasında farklılık göstermedi. ADE I/D gen polimorfizminin migren grubunda genotipik dağılımı kontrol grubundan anlamlı olarak farklı bulundu. DD ve ID genotiplerinin II genotipine göre migren olasılığını 2,571 (%95 CI- 1,138-5,811) ile 4,453 (%95 CI- 2,006-9,883) oranında artırdığı saptandı. Aynı risk artışı auralı migren alt grubunda her iki genotip için sürerken, aurasız migren grubunda sadece ID genotipi için korundu (OR- 3,750, %95 CI- 1,493-9,420). Migren sıklığı ve şiddeti ile gen polimorfizmleri arasında ilişki gözlenmedi. So­nuç: Çalışmamız ADE I/D gen polimorfizmi ile migren ilişkisini desteklemiştir. Ancak eNOS 4 a/b gen polimorfizmi ile migren arasında ilişki gösterilememiştir. (Nö­rop­si­ki­yat­ri Ar­fli­vi 2013; 50: 274-278)Introduction: In this study, we investigated the association of migraine with the Variable Number of Tandem Repeats (VNTR), repeated as 27 base pair, gene polymorphism in intron 4 of the endothelial nitric oxide synthase (eNOS) and the insertion/deletion of angiotensin converting enzyme (ACE) gene polymorphisms. Met­hods: One hundred and five migraine and ninety seven healthy female control subjects were enrolled in the study. The patients were subdivided as migraine with aura and without aura, and the frequency and severity of migraine headaches were recorded. The eNOS VNTR (eNOS 4 a/b) and ACE insertion/deletion gene polymorphisms (ACE I/D) were assessed by polymerase chain reactions. Re­sults: The allele and genotype frequencies of eNOS 4 a/b gene polymorphism showed no difference between the migraine and control groups. The genotypic distribution of the ACE I/D gene polymorphism in the migraine group significantly differed from that in the control group . The DD and ID genotype increased the risk of migraine as much as 2.571 (95% CI-1.138-5.811) and 4.453 (95% CI-2.006-9.883) compared to the II genotype. The same increased risk sustained for both genotypes in the migraine with aura subgroup, but only the ID genotype remained as the risk factor in the migraine without aura subgroup (OR- 3.750, 95% CI- 1.493-9.420). No association of gene polymorphisms with migraine frequency and severity was observed. Conc­lu­si­on: Our findings support the relationship between migraine and the ACE I/D gene polymorphism. However, no association was found between migraine and the eNOS 4 a/b gene polymorphism. (Arc­hi­ves of Neu­ropsy­chi­atry 2013; 50: 274-278

Soluble CD40 ligand and prolactin levels in migraine patients during interictal period

The relationship of migraine with cardiovascular diseases has been clarified by many studies, and currently, migraine is suggested to be a systematic vasculopathy. Inflammation, thrombosis and impaired vascular reactivity are the underlying pathophysiological mechanisms of the vasculopathy. In the present study, we aimed to investigate the relationship between prolactin levels and subclinical atherosclerosis risk factors such as soluble CD40 ligand (sCD40L) and high-sensitivity CRP (hsCRP) in migraine patients during interictal period. Fifty female migraine patients and age-matched 25 female control cases were enrolled in the study. Migraine diagnosis was settled according to the ICHD-II diagnostic criteria. A questionnaire was completed about the existence of vascular risk factors. Serum samples were used to measure sCD40L, hsCRP and prolactin levels. No difference was found between the prolactin levels of the migraine patients and the controls. The sCD40L levels were significantly higher in migraine patients (p < 0.001). High-sensitivity CRP levels showed no difference between the groups. There was no correlation between prolactin, sCD40L, and hs-CRP levels in migraine patients. We consider that the migraine patients are prone to subclinical atherosclerosis, but this tendency is independent of prolactin levels

Migraine in Metabolic Syndrome

Objectives: Recent studies suggest that insulin resistance is In ore common in patients with migraine. Insulin resistance underlies the pathogenesis of obesity, diabetes. and hypertension that are components of metabolic syndrome. As migraine is associated with an increased risk of vascular disorders, such as stroke. and migraine patients have higher diastolic blood pressure than healthy individuals, we aimed to investigate the I-year prevalence of migraine in metabolic syndrome

Epidemiologic investigation of amyotrophic lateral sclerosis in Trakya, Turkey, 2006-2010

22nd World Congress of Neurology (WCN) -- OCT 31-NOV 05, 2015 -- Santiago, CHILE[No Abstract Available

An epidemiologic investigation of amyotrophic lateral sclerosis in Thrace, Turkey, 2006-2010

Objective: The aim of the study was to investigate the incidence and prevalence of amyotrophic lateral sclerosis (ALS) in Thrace, Turkey in a five-year time period (2006-2010). Methods: Study population included residents of three provinces (Edirne, Tekirdag, Kirklareli) in the Thrace region. Cases were ascertained from all of the neurologic centers and hospitals of these provinces. Demographic and clinical information was collected for each patient. Newly diagnosed ALS patients who are fulfilling the El Escorial revised diagnostic criteria were enrolled into the study. Results: We identified a total of 145 patients (93 males, 52 females). The mean age at diagnosis was 57.0 +/- 13.6. According to El Escorial criteria, 60.0% of the cases were definite ALS, 24.8% were probable, and 15.2% were possible ALS. Thirty-two cases were bulbar (22.1%), 113 cases (77.9%) were spinal onset. Mean time delay from onset to diagnosis was 12.0 +/- 11.2 months. Age-gender standardized incidence rates with reference to Turkey, USA 2008 census were 1.9 (95% confidence interval (CI), 1.8-2.1), 1.9 (95%CI, 1.8-2.2) for overall. There were 112 living ALS patients at the end of the study. Crude point prevalence was calculated as 7.3 per 100,000 population (95%CI, 5.9-8.7). Conclusions: This is the first study to provide fundamental data about demographic and clinical characteristics about ALS in Thrace region of Turkey. Incidence and prevalence of ALS in Thrace region of Turkey appear to be comparable with European countries

Migren hastalari{dotless}nda endotelyal nitrik oksit sentaz ve anjiyotensin dönüştürücü enzim gen polimorfizmleri]

Introduction: In this study, we investigated the association of migraine with the Variable Number of Tandem Repeats (VNTR), repeated as 27 base pair, gene polymorphism in intron 4 of the endothelial nitric oxide synthase (eNOS) and the insertion/deletion of angiotensin converting enzyme (ACE) gene polymorphisms. Methods: One hundred and five migraine and ninety seven healthy female control subjects were enrolled in the study. The patients were subdivided as migraine with aura and without aura, and the frequency and severity of migraine headaches were recorded. The eNOS VNTR (eNOS 4 a/b) and ACE insertion/deletion gene polymorphisms (ACE I/D) were assessed by polymerase chain reactions. Results: The allele and genotype frequencies of eNOS 4 a/b gene polymorphism showed no difference between the migraine and control groups. The genotypic distribution of the ACE I/D gene polymorphism in the migraine group significantly differed from that in the control group. The DD and ID genotype increased the risk of migraine as much as 2.571 (95% CI-1.138-5.811) and 4.453 (95% CI-2.006-9.883) compared to the II genotype. The same increased risk sustained for both genotypes in the migraine with aura subgroup, but only the ID genotype remained as the risk factor in the migraine without aura subgroup (OR-3.750, 95% CI-1.493-9.420). No association of gene polymorphisms with migraine frequency and severity was observed. Conclusion: Our findings support the relationship between migraine and the ACE I/D gene polymorphism. However, no association was found between migraine and the eNOS 4 a/b gene polymorphism