Morphology and body weights of <i>Kcnj13</i> null mutant mice.

<p>a. Analysis of Kir7.1 expression in WT, heterozygous and null mutant mice; cyclophilin A (Cyc1) is used as constitutively expressed control gene. b. Gross morphology of WT, and heterozygous and homozygous <i>Kcnj13</i> null mutant newborn pups. c. Body weight vs. embryonic stage for WT (circles), <i>Kcnj13</i>^<i>+/-</i> (triangles), and <i>Kcnj13</i>^-/- (squares) embryos. Results are expressed as mean ± S.E.M. of the following numbers of embryos: 12.5 dpc: WT 3, <i>Kcnj13</i>^<i>+/-</i> 9, <i>Kcnj13</i>^-/- 4; 13.5 dpc: WT 9, <i>Kcnj13</i>^<i>+/-</i> 14, <i>Kcnj13</i>^-/- 2; n 14.5 dpc: WT 3, <i>Kcnj13</i>^<i>+/-</i> 11, <i>Kcnj13</i>^-/- 6; 15.5 dpc: WT 7, <i>Kcnj13</i>^<i>+/-</i> 20, <i>Kcnj13</i>^-/- 14; 16.5 dpc: WT 7, <i>Kcnj13</i>^<i>+/-</i> 5, <i>Kcnj13</i>^-/- 4; 17.5 dpc:WT 5, <i>Kcnj13</i>^<i>+/-</i> 12, <i>Kcnj13</i>^-/- 6; 18.5 dpc: WT 4, <i>Kcnj13</i>^<i>+/-</i> 5, <i>Kcnj13</i>^-/- 6; P0: WT 7, <i>Kcnj13</i>^<i>+/-</i> 15, <i>Kcnj13</i>^-/- 10. * p< 0.001; ** p <0.05 for the differences between <i>Kcnj13</i>^-/- and <i>Kcnj13</i>^<i>+/+</i> data (ANOVA).</p

Survival of <i>Kcnj13</i>^- mutant mice.

<p>Survival of <i>Kcnj13</i>^- mutant mice.</p

Basolateral expression of Kir7.1 channel in the epithelium of the airways.

<p>Immunohistochemical detection of Kir7.1 channel in trachea (left) and bronchiole (right) in adult <i>Kcnj13</i>^+/+, newborn <i>Kcnj13</i>^+/+ or newborn <i>Kcnj13</i>^-/- mice. Tissue sections were treated with anti-Kir7.1 antibody (1:15,000). Kir7.1 expression was restricted to the basolateral membrane of airway epithelium in adult and newborn <i>Kcnj13</i>^+/+ mice. Staining in <i>Kcnj13</i>^-/- tissues shows complete absence of specific immunoreactive signal. Nuclei were counterstained with Fast Red. Scale bar represents 50 μm.</p

Pulmonary abnormalities in embryonic lungs from <i>Kcnj13</i>^-/- mice.

<p>a. Hematoxylin and eosin stained lung sections taken at various gestational stages as indicated. Morphological differences in KO lungs were observed at E18.5 and P0. Null mutant mice show a lower air space and thicker walls at lung terminal sacs compared to WT and heterozygous mice. No differences were visible between <i>Kcnj13</i>^+/+ and <i>Kcnj13</i>^+/- genotypes. Scale bars represent 100 μm. b. Morphometric analysis of terminal sac spaces in lungs at various gestational stages. Significant reduction in spaces was observed in Kir7.1 deficient mice from E18.5 onwards. Results are expressed as mean ± S.E.M, # p<0.05 and * p<0.01 for the difference with WT by ANOVA. c. Graphical representation of newborn lung flotation test. Grey sections of columns correspond to percent of floating lungs, with black being the percent sinking lungs.</p

Increased Expression of <i>Lincpint</i> from Postnatal Day 3 to 8 Weeks of Age.

<p><i>LacZ</i> reporter expression (blue) at 3 days, 3 weeks, and 8 weeks in F0 heterozygotes shows increasing <i>Lincpint</i> expression with age. (A) At 3 days, ßgalactosidase staining is only observed in portions of the brain, tendons and ligaments of the hind limb, and some bronchioles in the lung (arrow). (B) At 3 weeks, there is increased staining in the brain, hindlimb, atria of the heart, lung, and liver. (C) By 8 weeks of age, the whole brain, skeletal muscle of the hindlimb and chest, atria and myocardium, lung, and liver tissue all exhibit strong ß-galactosidase staining representative of increased <i>Lincpint</i> expression. Examples shown are representative of n>4 mice per group. (D) RT-PCR analysis of <i>GFP</i> in GA (gastrocnemius) muscle and kidney isolated from 8 week-old and 52 week-old wild type (WT), heterozygous (<i>Lincpint-GFP</i>^{<i>+⁄−</i>}) and homozygous (<i>Lincpint-GFP</i>^{<i>−⁄−</i>}) mice. (E) Comparison of endogenous <i>Lincpint</i> RNA level in GA (gastrocnemius) muscle and kidney of 8 week-old versus 52 week-old wild type (WT) tissues demonstrating increased in <i>Lincpint</i> gene expression. Examples shown are representative of n>4 mice per group.</p

<i>LacZ</i> Reporter Expression in Brains of 6–8 Week Old lincRNA Gene-Targeted F0 Generation Heterozygotes.

<p>The brain <i>lacZ</i> expression pattern (blue) for each lincRNA gene is as follows: (A<b>)</b><i>Crnde</i>, the colliculi (dorsal view, arrow); (B) <i>Pantr1</i>, neocortex, olfactory bulb, basal forebrain, and hypothalamus; (C<b>)</b><i>Pantr2</i>, neocortex, olfactory bulb, cerebellum, hypothalamus, and basal forebrain; (D<b>)</b><i>Lincenc1</i>, neocortex, parts of the cerebellum and medial hypothalamus, especially strong patterning in the olfactory projection and olfactory projection areas of the temporal cortex (ventral view, red arrow); (E<b>)</b><i>Celrr</i>, broadly in gray matter with the exception of the lateral cerebellum and ventral pons; (F) <i>Kantr</i>, possibly in deep cerebellar layers (dorsal view, star); (G<b>)</b><i>Lincpint</i>, ubiquitously in gray matter, especially intense in the hypothalamus; (H<b>)</b><i>Lincppara</i>, ubiquitously in gray matter, especially dense in the hypothalamus; (I<b>)</b><i>Peril</i>, midline of the hypothalamus (ventral view, arrowhead); and (J) <i>Tug1</i>, spinal cord gray matter and light gray matter in most structures except for the neocortex. n = 2, genotype confirmed male mice per lincRNA knockout project.</p

Aging-associated Phenotypes in <i>Lincpint</i> Knockout Mice.

<p>(A) <i>Lincpint</i>^{<i>−⁄−</i>} and <i>Lincpint</i>^{<i>+⁄−</i>} male mice exhibit a significantly slower growth rate than their wild type (WT) littermates and begin to show significant weight loss near 6 months of age. Data are plotted as the mean +/− SEM, n > 9 mice for each group. Significance was assessed by a one-way ANOVA (*, <i>P</i> < 0.05; **, <i>P</i> < 0.005; ***, <i>P</i> < 0.001). (B) Kaplan-Meier analysis of homozygous with heterozygous and WT mice. <i>Lincpint</i>^{<i>−⁄−</i>} male mice exhibit a significant reduction in survival compare to <i>Lincpint</i>^{<i>+⁄−</i>} and wild type littermates. Data are plotted as percent survival over 1 year observation. (C) Ventral and dorsal skin sections in <i>Lincpint</i>^{<i>−⁄−</i>} mice compared with <i>Lincpint</i>^{<i>+⁄−</i>} and WT littermates. (D, E, F, and G) MicroCT evaluation of body composition at 12-, 26- and 52-weeks of age. (D, E) Male <i>Lincpint</i>^{<i>−⁄−</i>} and <i>Lincpint</i>^{<i>+⁄−</i>} mice exhibit a significant reduction in body fat as early as 26-week of age. Female <i>Lincpint</i>^{<i>−⁄−</i>} mice have reduced body fat at an older age noticeably at 52-week of age (***, <i>P</i> < 0.001, one-way ANOVA). (F, G) A significant reduction in femur bone mineral density (BMD) observed in both males and females <i>Lincpint</i>^{<i>−⁄−</i>} compared with their <i>Lincpint</i>^{<i>+⁄−</i>} and WT littermates (*, <i>P</i> < 0.05; ***, <i>P</i> < 0.001, one-way ANOVA). (H) MicroCT images depict pronounced lordokyphosis (curvature of the spinal column) seen in older male and female <i>Lincpint</i>^{<i>−⁄−</i>} mice compared with WT littermates. (I) Approximately 70% (6/9 males and 7/10 females) of <i>Lincpint</i>^{<i>−⁄−</i>} mice have lordokyphosis by 12 weeks of age, compared with 0–20% of <i>Lincpint</i>^{<i>+⁄−</i>} (1/12 males and 2/10 females) and WT (1/10 males and 0/11 females) littermates. By 26 weeks of age the proportion of <i>Lincpint</i>^{<i>−⁄−</i>} mice with lordokyphosis increased to nearly 90% (7/8 males and 8/9 females) and appeared in approximately 60% (8/12 males and 6/10 females) of <i>Lincpint</i>^{<i>+⁄−</i>} mice, compared with less than 20% (2/10 males and 2/11 females) of WT littermates. n ≥ 9 mice per group for all observations reported.</p

Abnormal Hindlimb Posture, Reduced Grip Strength, and Muscle Wasting in <i>Hottip</i>^{<i>−⁄−</i>} Mice.

<p>(A) <i>Hottip</i>^{<i>−⁄−</i>} mice demonstrated unusual hindlimb clasping posture when suspended by the tail. (B) Cage endurance testing revealed that <i>Hottip</i>^{<i>−⁄−</i>} mice have a reduced ability to remain suspended from an inverted wire cage top, n = 5 mice for each group. (C) The right and left TA (tibialis anterior), GA (gastrocnemius) and Quad (quadriceps) muscles from WT, <i>Hottip</i>^{<i>+⁄−</i>} and <i>Hottip</i>^{<i>−⁄−</i>} mice were weighed. Muscle weights are normalized to body weight and calculated to include both right/left muscle weights. Data are means +/−SEM, n = 6 mice for each group. A significant decrease in muscle weight was observed only in the GA of <i>Hottip</i>^{<i>−⁄−</i>} animal in both males and females (male data not shown). Asterisks indicate a significant difference in the <i>Hottip</i>^{<i>−⁄−</i>} GA muscle weights compared to all other control groups (P< 0.01). (D) Comparison of GA muscle fiber numbers in WT, <i>Hottip</i>^{<i>+⁄−</i>} and <i>Hottip</i>^{<i>−⁄−</i>}. A significant reduction of fiber count was observed in <i>Hottip</i>^{<i>−⁄−</i>}. Significance assessed by one-way ANOVA (P < .0001 (E) Comparison of mean cross-sectional area of muscle fibers. Cross sections taken from the GA muscle were stained with an antibody against laminin and measured. There is no noticeable size difference between <i>Hottip</i>^{<i>−⁄−</i>} and control skeletal muscles, n = 6 mice per group for all muscle analyses.</p

Abnormal Lung Morphology in <i>Fendrr</i> Knockout Mice at E13.5.

<p>(A) <i>LacZ</i> reporter gene expression at E12.5 in <i>Fendrr</i>^{<i>−⁄−</i>} embryos in the frontonasal region (FN) of the face, the aorta gonad mesonephros (AGM) region, and the respiratory tract, including the lungs (L) and trachea (T). (B) Dissection of lungs at E13.5 revealed an abnormal, disorganized, globular phenotype in the lobes of <i>Fendrr</i>^{<i>−⁄−</i>} embryos compared with <i>Fendrr</i>^{<i>+⁄−</i>}.</p

Homeotic Transformation Observed in the 4th Caudal Vertebra of <i>Hotair</i>^{<i>−⁄−</i>} mice.

<p>(A) Visualization of the sacral and caudal region of the mouse skeleton by microCT reveals a homeotic transformation in <i>Hotair</i>^{<i>−⁄−</i>} mice of the 4th caudal vertebra to a structure similar to that of the 3rd caudal vertebra. (B) Dorsal, lateral and ventral comparison of WT and <i>Hotair</i>^{<i>−⁄−</i>} 4th caudal vertebra reveals a structural abnormality in homozygotes indicative of a homeotic transformation.</p