1 research outputs found
LC–MS-Based Urinary Metabolite Signatures in Idiopathic Parkinson’s Disease
Increasing
evidence has shown that abnormal metabolic phenotypes
in body fluids reflect the pathogenesis and pathophysiology of Parkinson’s
disease (PD). These body fluids include urine; however, the relationship
between, specifically, urinary metabolic phenotypes and PD is not
fully understood. In this study, urinary metabolites from a total
of 401 clinical urine samples collected from 106 idiopathic PD patients
and 104 normal control subjects were profiled by using high-performance
liquid chromatography coupled to high-resolution mass spectrometry.
Our study revealed significant correlation between clinical phenotype
and urinary metabolite profile. Metabolic profiles of idiopathic PD
patients differed significantly and consistently from normal controls,
with related metabolic pathway variations observed in steroidogenesis,
fatty acid beta-oxidation, histidine metabolism, phenylalanine metabolism,
tryptophan metabolism, nucleotide metabolism, and tyrosine metabolism.
In the fruit fly <i>Drosophila melanogaster</i>, the alteration
of the kynurenine pathway in tryptophan metabolism corresponded with
pathogenic changes in the alpha-synuclein overexpressed <i>Drosophila</i> model of PD. The results suggest that LC–MS-based urinary
metabolomic profiling can reveal the metabolite signatures and related
variations in metabolic pathways that characterize PD. Consistent
PD-related changes across species may provide the basis for understanding
metabolic regulation of PD at the molecular level