The immunomodulatory effect of silver nanoparticles is critical for promotion of tendon repair

Poster Session 1: abstract no. 486INTRODUCTION: Full recovery of tendon injury still remains difficult to achieve in modern medicine. Scarring and reduced mechanical properties of the repaired tendon are always the issues for tendon injury. Previously we demonstrated that local injection of silver nanoparticles (AgNPs) in a rat tendon rupture model successfully alleviated inflammation, improved collagen alignment and the tensile property of the repaired tendon at an early time point of 6 weeks [1]. In this study, we would like to investigate the mechanism behind. In the literatures, it was proven that there is a strong link between inflammation and tissue fibrosis in healing [2-6]. In tissue ...postprin

The development, fabrication, and material characterization of polypropylene composites reinforced with carbon nanofiber and hydroxyapatite nanorod hybrid fillers

published_or_final_versio

Polypropylene biocomposites with boron nitride and nanohydroxyapatite reinforcements

published_or_final_versio

Stringent requirement for spatial arrangement of extracellular matrix in supporting cell morphogenesis and differentiation

BACKGROUND: In vitro experiments on the functional roles of extracellular matrix (ECM) components usually involve the culture of cells on surfaces coated with purified ECM components. These experiments can seldom recuperate the spatial arrangement of ECM found in vivo. In this study, we have overcome this obstacle by using histological sections of bovine Achilles tendon as cell culture substrates. RESULTS: We found that tendon sections can be viewed as a pre-formed block of ECM in which the collagen fibrils exhibited a spatial regularity unraveled in any artificially constructed scaffold. By carving the tendon at different angles relative to its main axis, we created different surfaces with distinct spatial arrangements of collagen fibrils. To assess the cellular responses to these surfaces, human mesenchymal stem cells (MSCs) were directly cultured on these sections, hence exposed to the collagen with different spatial orientations. Cells seeded on longitudinal tendon sections adopted a highly elongated and aligned morphology, and expressed an increased level of tenomodulin, suggesting that the collagen fibrils present in this section provide a microenvironment that facilitates cell morphogenesis and differentiation. However, MSC elongation, alignment and induction of tenomodulin diminished dramatically even as the sectioned angle changed slightly. CONCLUSION: Our results suggest that cell functions are influenced not only by the type or concentration of ECM components, but also by the precise spatial arrangements of these molecules. The method developed in this study offers a simple and robust way for the studying of cell-ECM interactions, and opens many research avenues in the field of matrix biology.published_or_final_versio

Effects of long pulse width and high pulsing frequency on surface superhydrophobicity of polytetrafluoroethylene in quasi-direct-current plasma immersion ion implantation

Long pulse, high frequency quasi-direct-current (dc) oxygen plasma immersion ion implantation (PIII) is utilized to create a superhydrophobic polytetrafluoroethylene (PTFE) surface with a water contact angle of over 150°. This technique allows the use of a high duty cycle without deleterious effects such as extensive sample heating encountered in conventional PIII. Scanning electron microscopy images review submicrometer-nanometer structures on the PTFE surface after long pulse, high frequency PIII indicative of ion implantation. On the other hand, plasma modification is the dominant effect in short pulse, low frequency PIII. Quasi-dc PIII is demonstrated to offer adjustable synergistic plasma and ion beam effects. © 2009 American Institute of Physics.published_or_final_versio

The mechanical and biological properties of a novel biodegradable polycaprolactone-magnesium porous scaffold for bone tissue engineering

Session: Biomaterial-based Tissue Regeneration: abstract no. 150INTRODUCTION: Bone tissue engineering offers an alternative solution to the traditional methods of bone replacement including allografts and autografts. Tissue grafting has been used since 1660s1. However, there are concerns of tissue shortage and transmission of disease. Therefore, the use of scaffold is the most common technique and good approach to regenerate diseased or damaged bone tissue. Polycaprolactone (PCL) is one of the suitable candidates to be used as the scaffold material since it has a low degradation rate when compared with other polymers. However, the low mechanical strength and intrinsic …postprintThe 2010 North America Conference of the Tissue Engineering and Regenerative Medicine International Society (TERMIS-NA 2010), Orlando, FL., 5-8 December 2010

Impact of trp2 allele mutation of α2 chain in collagen IX on the structural integrity of human annulus fibrosus

Conference Theme: Spinal Motion Segment: From Basic Science to Clinical Applicationpublished_or_final_versio

Enhanced cell adhesion and proliferation on dual plasma modified titanium surfaces

Session: Biomaterials Fabrication and Synthesis: abstract no. 47INTRODUCTION: Ti-6Al-4V is widely used in present orthopedic applications, owing to a combination of good mechanical properties and excellent corrosion resistance. However, long-term success of Ti-6Al-4V implants and the completeness of their osteointegration still need to be addressed [1]. Since the biofunctionality of the implant is strongly affected by its surface characteristics, to promote osteointegration, considerable efforts have focused on modifying the surface of the implants. Alternatively, surface modification using plasma immersion ion implantation (PIII) has been developed in order to incorporate new biofunctional groups onto titanium alloy surfaces [2]. The present study aims at investigating the impact of carbon-nitrogen (Car-Nit) dual plasma surface treatment on …postprintThe 2010 North America Conference of the Tissue Engineering and Regenerative Medicine International Society (TERMIS-NA 2010), Orlando, FL., 5-8 December 2010

Improving quality of life through reducing post-operative bacterial infections

Session: Functionalization of Cells and Biomaterials: abstract no. 483INTRODUCTION: Metallic implants such as titanium alloy have been widely used in orthopaedic surgeries1. 2-5% of the patients acquire post-operative bacterial infections at implant site though prescription of antibiotics2. Patients may suffer from surgeries like debridement of wound, removal of problematic implant and insertion of new implant. To reduce bacterial infections and improve quality of life of patients, implant surfaces loaded with …postprintThe 2010 North America Conference of the Tissue Engineering and Regenerative Medicine International Society (TERMIS-NA 2010), Orlando, FL., 5-8 December 2010

Engineered magnesium-based resorbable porous scaffold for bone tissue engineering

INTRODUCTION: Bone tissue engineering offers an alternative solution to the traditional methods of bone replacement including allografts and autografts [1]. Although these biological materials possess good osteoinductive and osteoconductive properties, both of them have limitations in terms of the availability, donor site morbidity and the risk of disease transmission with the use of allografts [2]. Therefore, the use of synthetic scaffold is the most common technique and good approach to regenerate diseased or damaged bone tissue. An ideal bone substitute should possess certain properties including ...postprin