Different four PCR-multiplex systems via twentymicrosatellite loci in goat

European Biotechnology Congress -- SEP 28-OCT 01, 2011 -- Istanbul, TURKEY[No Abstract Available]European Biotechnol Themat Network Asso

Different four PCR-multiplex systems via twenty microsatellite loci in goat

European Biotechnology Congress -- SEP 28-OCT 01, 2011 -- Istanbul, TURKEY[No Abstract Available]European Biotechnol Themat Network Asso

Determination of microsatellite polymorphism in goat species using different PCR-multiplex systems

Bu çalışma; TÜBİTAK tarafından TÜRKHAYGEN-I (Türkiye Yerli Evcil Hayvan Genetik Kaynaklarından Bazılarının In Vitro Korunması ve Ön Moleküler Tanımlanması-I) projesi kapsamında desteklenmiştir. Mikrosatellit belirteçler ile genetik çeşitliliğin araştırılması amacıyla keçi türünde 20 lokustan oluşan 4 farklı çoklu Polimeraz Zincir Reaksiyonu (PZR) sisteminin kullanılabilirliği ortaya konulmaya çalışılmıştır. Çalışmanın materyalini Türkiye’de yetiştirilen Ankara Keçisi, Kilis Keçisi, Honamlı Keçisi, Kıl Keçisi ve Norduz Keçisi gibi 5 farklı yerli keçi ırkı oluşturmuştur. Araştırmada 20 lokusa ilişkin tek tek PZR optimizasyonlarının yapılmasının yanı sıra; 4 farklı çoklu PZR sisteminin de optimizasyonları yapılmış; bu sistemlerin populasyon yapısı ve genetik çeşitliliğin araştırılmasında kullanılabilirliği vurgulanmıştır.This study was supported by TUBITAK TURKHAYGEN-I (In Vitro Conservation and Preliminary Molecular Identification of Some Turkish Domestic Animal Genetic Resources-I) project. In order to investigate the genetic diversity of goat has been carried out with 20 different microsatellite markers in 4 different multi-locus polymerase chain reaction (PCR) system. Five different breed of domestic goat were used in the study namely Angora goat, Kilis goat, Honamlı goat, Hair goat and Norduz goat. PCR optimizations were made for individual locus and 4 different multiplex-PCR systems. The applicability of these systems were demonstrated for population structure and genetic diversity

Expression of Toll-Like Receptors in the Lung Tissue of Mouse Fetuses Generated by in vitro Embryo Culture and Embryo Transfer

Mouse fetuses generated by in vitro embryo culture and embryo transfer exhibit impaired lung development, altered composition of pulmonary epithelial cells associated with downregulation of several genes involved in lung development and toll-like receptor (TLR) signaling pathway. The aims of the present study were to determine the expression of all TLRs and to examine if the expression of TLRs, along with genes involved in TLR signaling pathway, is altered in the lung tissue of mouse fetuses generated through embryo culture and embryo transfer. Two experimental (EGs) and one control (CG) group were included in the study. Embryos cultured at 5% CO2-95% air for 95 h or less than 24 h were transferred to pseudo-pregnant females to obtain fetuses comprising EG(in vitro) (n = 18) and EG(in vivo) (n = 18), respectively. Fetuses obtained from naturally ovulating females on day 18 of pregnancy served as the CG (n = 18). Western blot and immunohistochemistry were used to determine the expression of TLR proteins. The expression of transcripts encoding TLRs, and the genes involved in TLR signaling pathway (Lbp, Pik3r1, Pik3cb, Nfkbia, and Fos), was determined using qRT-PCR. While all TLRs were expressed by cells lining the bronchial/bronchiolar epithelium of lung tissues in all groups, some of the TLRs were expressed in a specific pattern. When compared to CG, the expression of transcripts encoding TLR-2, -3, -4, -5, -7, -8, -9, -12, -13, Lbp, Pik3r1, Pik3cb, Nfkbia, and Fos was significantly downregulated in both EGs. It appears that stress imposed on embryos at preimplantation stages of development is associated with downregulation of TLRs, along with some of the genes involved in TLR signaling pathway, in the lung tissue during the perinatal period. It remains to be determined if downregulation of TLRs, along with the genes involved in TLR signaling pathway, has any functional consequences in the adult lung tissue.This work was funded by The Scientific and Technological Research Council of Turkey (TUBITAK, Grant No. 112O259).Scientific and Technological Research Council of Turkey (TUBITAK) [112O259