TaqMan real-time PCR for detection and quantitation of squash leaf curl virus in cucurbits

A real-time PCR assay based on the TaqMan chemistry was developed for reliable detection and quantitation of the squash leaf curl virus (SLCV) in melon and squash plants. This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. The protocol of the real-time PCR established in this study enabled detection of as little as 10(2) copies of SLCV DNA with CP gene as the target. This TaqMan real-time PCR assay for detection and quantitation of SLCV would be a useful tool for application in quarantine and certification of SLCV in cucurbits as well as in the research of disease resistance and epidemiology. (C) 2011 Elsevier B.V. All rights reserved

High-sensitivity microwave vector detection at extremely low-power levels for low-dimensional electron systems

We present a high-sensitivity microwave vector detection system for studying the low-dimensional electron system embedded in the gaps of a coplanar waveguide at low temperatures. Using this system, we have achieved 0.005% and 0.001degrees resolutions in amplitude and phase variations, respectively, at 10 GHz in a magnetotransport measurement on a quantum-wire array with an average signal power less than -75 dBm into the sample at 0.3 K. From the measured phase variation, we can distinguish a very tiny change in the induced dipole moment of each quantum wire. (C) 2004 American Institute of Physics

Cathepsin S mediates gastric cancer cell migration and invasion via a putative network of metastasis-associated proteins

10.1021/pr100492xJournal of Proteome Research994767-4778JPRO

Hong Kong identity and the press–politics dynamics: a corpus-assisted discourse study

This is an Author's Original Manuscript of an article submitted for consideration in the Asian Journal of Communication [Taylor and Francis © AMIC/SCI-NTU]. Asian Journal of Communication is available online at http://www.tandfonline.com/ and the definitive version is available at: http://dx.doi.org/10.1080/01292986.2012.701315This article uses corpus-assisted discourse studies to examine the discursive construction of collective identity in the Hong Kong press, particularly with regard to its relationship with mainland China. Drawing on critical discourse analysis and collective identity theory, it develops a set of analytical techniques amenable to quantification, and applies it to a corpus of newspaper coverage of the 2005 Hong Kong Chief Executive election. The article uses these techniques not only to develop a representative description and interpretation of discursive patterns, but also to offer an explanatory account of the discursive construction of Hong Kong identity. It shows that the discursive patterns vary systematically depending on newspaper ownership, commercial imperatives, and newspaper type