15 research outputs found
Regulation of RuBP carboxylase activity associated with photo-inhibition of wheat
Detached wheat leaves were illuminated in air until a steady rate of photosynthesis was established. Then the gas was changed to 1% O2, 99% N2 and after 2.5 h further illumination the capacity of the leaves for photosynthesis in air was decreased to approximately 50%. Measurement of RuBP carboxylase activity in extracts showed that inhibition of photosynthesis was accompanied by 70% inactivation of this enzyme. The capacity for photosynthesis and the activity of RuBP carboxylase were recovered when leaves were returned to normal air. Extracts of the leaves made when photosynthesis and carboxylase activity were low, recovered most of the lost carboxylase activity when supplemented with bicarbonate and magnesium ions. The time courses for activation and inactivation of the RuBP carboxylase in these experiments suggests the operation of a mechanism that has not yet been elucidated.
RESP-850