A case-control analysis of common variants in GIP with type 2 diabetes and related biochemical parameters in a South Indian population

<p>Abstract</p> <p>Background</p> <p>Glucose-dependent insulinotropic polypeptide (GIP) is one of the incretins, which plays a crucial role in the secretion of insulin upon food stimulus and in the regulation of postprandial glucose level. It also exerts an effect on the synthesis and secretion of lipoprotein lipase, from adipocytes, important for lipid metabolism. The aim of our study was to do a case-control association analysis of common variants in <it>GIP </it>in association with type 2 diabetes and related biochemical parameters.</p> <p>Method</p> <p>A total of 2000 subjects which includes 1000 (584M/416F) cases with type 2 diabetes and 1000 (470M/530F) normoglycemic control subjects belonging to Dravidian ethnicity from South India were recruited to assess the effect of single nucleotide polymorphisms (SNPs) in <it>GIP </it>(rs2291725, rs2291726, rs937301) on type 2 diabetes in a case-control manner. The SNPs were genotyped by using tetra primer amplification refractory mutation system-PCR (ARMS PCR). For statistical analysis, our study population was divided into sub-groups based on gender (male and female). Association analysis was carried out using chi-squared test and the comparison of biochemical parameters among the three genotypes were performed using analysis of covariance (ANCOVA).</p> <p>Result</p> <p>Initial analysis revealed that, out of the total three SNPs selected for the present study, two SNPs namely rs2291726 and rs937301 were in complete linkage disequilibrium (LD) with each other. Therefore, only two SNPs, rs2291725 and rs2291726, were genotyped for the association studies. No significant difference in the allele frequency and genotype distribution of any of the SNPs in <it>GIP </it>were observed between cases and controls (<it>P </it>> 0.05). Analysis of biochemical parameters among the three genotypes showed a significant association of total cholesterol (<it>P </it>= 0.042) and low density lipoprotein (LDL) with the G allele of the SNP rs2291726 in <it>GIP </it>(<it>P </it>= 0.004), but this was observed only in the case of female subjects. However this association does not remain significant after correction for multiple testing by Bonferroni's inequality method.</p> <p>Conclusion</p> <p>No statistically significant association was observed between any of the SNPs analysed and type 2 diabetes in our population. But the analysis of biochemical parameters indicates that the G allele in rs2291726 may be a putative risk allele for increased LDL cholesterol and further studies in other population needs to be carried out for ascertaining its role in cholesterol metabolism and subsequent cardiovascular risk.</p

A Survey of Bayesian Statistical Approaches for Big Data

The modern era is characterised as an era of information or Big Data. This has motivated a huge literature on new methods for extracting information and insights from these data. A natural question is how these approaches differ from those that were available prior to the advent of Big Data. We present a review of published studies that present Bayesian statistical approaches specifically for Big Data and discuss the reported and perceived benefits of these approaches. We conclude by addressing the question of whether focusing only on improving computational algorithms and infrastructure will be enough to face the challenges of Big Data

Multi-ancestry genome-wide association meta-analysis of Parkinson’s disease

\ua9 2023, This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply. Although over 90 independent risk variants have been identified for Parkinson’s disease using genome-wide association studies, most studies have been performed in just one population at a time. Here we performed a large-scale multi-ancestry meta-analysis of Parkinson’s disease with 49,049 cases, 18,785 proxy cases and 2,458,063 controls including individuals of European, East Asian, Latin American and African ancestry. In a meta-analysis, we identified 78 independent genome-wide significant loci, including 12 potentially novel loci (MTF2, PIK3CA, ADD1, SYBU, IRS2, USP8, PIGL, FASN, MYLK2, USP25, EP300 and PPP6R2) and fine-mapped 6 putative causal variants at 6 known PD loci. By combining our results with publicly available eQTL data, we identified 25 putative risk genes in these novel loci whose expression is associated with PD risk. This work lays the groundwork for future efforts aimed at identifying PD loci in non-European populations

Synergistic typhoid and taenia solium intestinal perforation.

Typhoid and intestinal infestation with parasites are common and may co-exist especially in the tropics. Their synergistic acute abdominal presentation is rare. We here report a 38-year-old male who presented with complaints suggestive of enteric fever and signs of peritonitis. Following baseline investigations, laparotomy was performed which proved to be a surgical surprise. A live tape worm was seen pouting out of the terminal ileal perforation. The clinical presentation and the stringent attention to preventive measures are discussed

Jejunal perforation due to single ascaris lumbricoides – a case report.

Ascriasis is a common infection in underdeveloped and developing countries and usually detected incidentally on evaluation of nonspecific pain abdomen. Complications related to ascariasis occur mainly due to heavy worm load. We here report a rare case, in which a single live ascaris worm had caused jejunal perforation

Effect of bipolar pulsed voltage on properties of DLC films deposited by inductivity coupled PECVD

Diamond-like carbon (DLC) is a metastable form of amorphous carbon having large sp3 bonding. DLC films have unique properties such as high hardness, low-friction, good transparency in IR region, chemical inertness and biocompatibility. To have better performance of the coating one should have proper selection of different parameters for deposition. In the present study, DLC films were deposited on p-type silicon (100) substrates using methane (CH4) and hydrogen (H2) as source gases in an inductively coupled RF plasma CVD system. Bipolar pulsed bias voltage source at 10 KHz frequency used for substrate shows proper DLC film. The DLC films were characterized for surface morphology & roughness, hardness & Young’s modulus, IR absorption and micro-Raman spectroscopy. Deposited DLC film images show that the roughness decreases, hardness & Young’s modulus increases and the Id/Ig ratio decreases with the increase of pulse bipolar bias voltage. Raman result shows that disorder decreases. The correlation plotted between Id/Ig, hardness and roughness