13 research outputs found
Introgression and isolation contributed to the development of Hungarian Mangalica pigs from a particular European ancient bloodline
Developmental competence of antral follicles and their oocytes after gonadotrophin treatment of sows with gene polymorphisms for leptin and melanocortin receptors (Iberian pig)
Purpose To evaluate possible differences in follicle and oocyte developmental competence after gonadotrophin treatment in sows of obese and lean genotypes. Methods Follicle dynamics, ovulation rate and oocyte developmental competence to embryo were compared between females, of obese (nâ=â7) and lean genotypes (nâ=â10), treated with 1,250 I.U. of eCG and 500 I.U. of hCG. Results The obese genotype showed lower numbers of follicles growing to preovulatory stages (12.4â±â1.8 vs 18.6â±â1.0, Pâ<â0.05), of corpora lutea (16.0â±â0.9 vs 23.5â±â0.9, Pâ<â0.05), and of recovered oocytes/embryos (8. 0â±â1.3 vs 12.9â±â0.9, Pâ<â0.05). Thereafter, embryo viability rates also decreased when compared to lean genotypes (62.5 vs 77.6%, Pâ<â0.05). Discussion To our knowledge, this is the first study analyzing the effect of obese genotypes on the ovarian response to exogenous gonadotrophins in a non-rodent animal model, the pig. A lower efficiency of gonadotrophin treatments for stimulation of follicle development and induction of ovulation was observed. © 2011 Springer Science+Business Media, LLC
Physiological Function of Rac Prophage During Biofilm Formation and Regulation of Rac Excision in Escherichia coli K-12
Disentangling the relative influence of bacterioplankton phylogeny and metabolism on lysogeny in reservoirs and lagoons
Previous studies indicate that lysogeny is preponderant when environmental conditions are challenging for the bacterial communities and when their metabolism is reduced. Furthermore, it appears that lysogeny is more frequent within certain bacterial phylogenetic groups. In this comparative study from 10 freshwater reservoirs and 10 coastal lagoons, we aim to disentangle the influence of these different factors. In eight reservoirs and four lagoons, lysogeny was detected by induction assays with mitomycin C, and induction significantly modified the bacterial community composition (BCC), whereas community composition remained constant in ecosystems in which lysogeny was not observed. Among the phylogenetic groups studied, the most abundant ones were Bacteroidetes and α-proteobacteria in lagoons, and ÎČ-proteobacteria and Bacteroidetes in reservoirs. These dominant groups comprised the highest proportions of inducible lysogens. In order to unravel the effects of bacterial metabolism from phylogeny on lysogeny, we measured bacterial community physiology and the specific activities of selected phylogenetic groups. The proportion of inducible lysogens within the α- and the ÎČ-proteobacteria decreased with increasing group-specific metabolism in lagoons and reservoirs, respectively. In contrast, this relationship was not observed for the other lysogen-containing groups. Hence, both host physiology and phylogeny are critical for the establishment of lysogeny. This study illustrates the importance of lysogeny among the most abundant phylogenetic groups, and further suggests its strong structuring impact on BCC