Functional KV10.1 Channels Localize to the Inner Nuclear Membrane

Ectopically expressed human KV10.1 channels are relevant players in tumor biology. However, their function as ion channels at the plasma membrane does not totally explain their crucial role in tumors. Both in native and heterologous systems, it has been observed that a majority of KV10.1 channels remain at intracellular locations. In this study we investigated the localization and possible roles of perinuclear KV10.1. We show that KV10.1 is expressed at the inner nuclear membrane in both human and rat models; it co-purifies with established inner nuclear membrane markers, shows resistance to detergent extraction and restricted mobility, all of them typical features of proteins at the inner nuclear membrane. KV10.1 channels at the inner nuclear membrane are not all transported directly from the ER but rather have been exposed to the extracellular milieu. Patch clamp experiments on nuclei devoid of external nuclear membrane reveal the existence of channel activity compatible with KV10.1. We hypothesize that KV10.1 channels at the nuclear envelope might participate in the homeostasis of nuclear K+, or indirectly interact with heterochromatin, both factors known to affect gene expression

Towards A Complete Detection/correction System

: In this paper we describe a system for detection and correction of lexical and syntactic errors in a French text. The lexical level uses several techniques (keys, phonetics and morphological generation). The aim of the syntactic level is verification of the structure of a sentence and of relations between words (e.g. agreement). It proceeds by unification of the decorations on the nodes of a dependency tree, by application of condition-action rules. By use of a morphological generator, corrections can be offered for agreement errors. We propose to integrate the two levels in a complete tool, based on a real size lexicon. Keywords : morphological parsing and generation, similarity keys, phonetics, syntactic parsing, syntactic verification, unification, concordancy correction 1. INTRODUCTION We are working on the development of an interactive system for detection and correction of errors on the lexical and the syntactic level in a written French language text. Our system is modular, an..