13 research outputs found
In Vitro Safety Assessment of the Effect of Five Medicinal Plants on Human Peripheral Lymphocytes
Purpose: To evaluate, using ethnomedical data approach, five Indian plants used in traditional medicine for cancer and other diseases for their safety and cytotoxic activity on human lymphocytes.Methods: The antiproliferative effect of the 50, 100 and 200 ìg/ml aqueous extracts of five plants (leaves of Phyllanthus niruri, Coleus aromaticus, Azadirachta indica and Camellia sinensis, and driedfruit rind of Garcinia indica) were evaluated in vitro using trypan blue dye exclusion and clonogenic assay methods on two cell lines - Balb/c 3T3 mouse fibroblasts and human peripheral lymphocytes.Results: Among the five plants used traditionally to treat cancer and other infections, two of them (A. indica and C. aromaticus) exhibited cytotoxic effects on lymphocytes. Two other plants (G. indica and P.niruri) exhibited pronounced cytotoxic effects. Another plant (Camellia sinensis) exhibitedcytostimulatory effect (> 50 % cell proliferation). For the plants that are traditionally used in anticancer therapy, there was a correlation between the reported use of these plants and their cytotoxic activity onlymphocytes.Conclusion: The plant extracts of the leaves of P. niruri, C, aromaticus and A. indica, and the dried fruit rind of G. indica are cytotoxic to lymphocytes and this lends some credence to their traditional use for cancer treatment. However, green tea (C. sinensis) is cytostimulatory and safe for consumption
Purification and Biochemical Characterization of pH Tolerant and Acid Stable á-amylase from Aspergillus oryzae JGI 21 Isolated from Soil
This paper describes the purification and characterization of a novel acid stable and pH tolerant α-amylase from a Aspergillusoryzae JGI
21 isolated from Mangalore. The enzyme displayed a molecular weight of 22 kDa and it was stable over a broad range of acidic and alkaline pH with maximum activity and stability at 6.5. The optimum temperature of enzyme stability was found to be around 24+/-2◦C. The purification of α-amylase by ammonium sulphate precipitation and ion-exchange chromatography resulted in 23.56 fold increase in its activity (100.38 U/mg protein). Considering its promising properties, this enzyme can find potential applications in the food industry as well as in laundry detergents
In Vitro Safety Assessment of the Effect of Five Medicinal Plants on Human Peripheral Lymphocytes
Purpose: To evaluate, using ethnomedical data approach, five Indian
plants used in traditional medicine for cancer and other diseases for
their safety and cytotoxic activity on human lymphocytes. Methods: The
antiproliferative effect of the 50, 100 and 200 µg/ml aqueous
extracts of five plants (leaves of Phyllanthus niruri, Coleus
aromaticus, Azadirachta indica and Camellia sinensis, and dried fruit
rind of Garcinia indica) were evaluated in vitro using trypan blue dye
exclusion and clonogenic assay methods on two cell lines -Balb/c 3T3
mouse fibroblasts and human peripheral lymphocytes. Results: Among the
five plants used traditionally to treat cancer and other infections,
two of them (A. indica and C. aromaticus) exhibited cytotoxic effects
on lymphocytes. Two other plants (G. indica and P. niruri) exhibited
pronounced cytotoxic effects. Another plant (Camellia sinensis)
exhibited cytostimulatory effect (> 50 % cell proliferation). For
the plants that are traditionally used in anticancer therapy, there was
a correlation between the reported use of these plants and their
cytotoxic activity on lymphocytes. Conclusion: The plant extracts of
the leaves of P. niruri, C, aromaticus and A. indica, and the dried
fruit rind of G. indica are cytotoxic to lymphocytes and this lends
some credence to their traditional use for cancer treatment. However,
green tea (C. sinensis) is cytostimulatory and safe for consumption