Impacts of Bacillus thuringiensis var. israelensis and Bacillus sphaericus insect larvicides on mosquito larval densities in Lusaka, Zambia

The study assessed the impact of bio-larvicides- Bacillus thuringiensis var. israelensis (Bti) and B. sphaericus (Bs) on anopheline mosquito larval densities in four selected areas of Lusaka urban district. Larval densities were determined using a standard WHO protocol at each study area prior to and after larviciding. Ninety percent (90%) of the collected mosquito larvae and pupae were preserved in 70% ethanol, while 10% were reared to adults for species identification. Prior to larviciding, the largest number of mosquito larvae collected was culicines. Among the anophelines, Anopheles coustani Laveran (13.5%) (n = 111) and An. squamosus Theobald (9.5%) (n = 78) were identified from all the study areas with An. rufipes Gough (1.1%) (n = 9) collected from one study area only. None of the major malaria vector species reported for Zambia were identified. No mosquito larvae were found in freshwater bodies following the larviciding exercise. Possible reasons for the absence of known major malaria vectors could be the re-introduction of effective vector control and loss of suitable breeding grounds. The study highlights the potential of larviciding using Bti and Bs for malaria vector control and its integration with indoor residual spraying and insecticide treated nets

Sounds, Behaviour, and Auditory Receptors of the Armoured Ground Cricket, Acanthoplus longipes

The auditory sensory system of the taxon Hetrodinae has not been studied previously. Males of the African armoured ground cricket, Acanthoplus longipes (Orthoptera: Tettigoniidae: Hetrodinae) produce a calling song that lasts for minutes and consists of verses with two pulses. About three impulses are in the first pulse and about five impulses are in the second pulse. In contrast, the disturbance stridulation consists of verses with about 14 impulses that are not separated in pulses. Furthermore, the inter-impulse intervals of both types of sounds are different, whereas verses have similar durations. This indicates that the neuronal networks for sound generation are not identical. The frequency spectrum peaks at about 15 kHz in both types of sounds, whereas the hearing threshold has the greatest sensitivity between 4 and 10 kHz. The auditory afferents project into the prothoracic ganglion. The foreleg contains about 27 sensory neurons in the crista acustica; the midleg has 18 sensory neurons, and the hindleg has 14. The auditory system is similar to those of other Tettigoniidae

Signaling or Not-Signaling: Variation in Vulnerability and Defense Tactics of Armored Ground Crickets (Acanthoplus Speiseri: Orthoptera, Tettigoniidae, Hetrodinae)

Male Orthoptera singing from exposed perches are at risk from acoustically- and visually-hunting predators. The defensive reactions of armored ground crickets (Acanthoplus speiseri) include falling silent, dropping from their perch, alarm stridulation and autohaemorrhaging. Male and female ground crickets show different reactivity (i.e. the number or intensity of defense tactics used) to predation, depending on level of exposure: calling males were more reactive when approached during daylight, compared with in the dark. During daylight, calling males were more reactive than silent, cryptic, males and females. The level of response presumably reflected the riskiness of the individual’s behavior and situation at that time. Plasticity of response to predation allows individuals to balance risky behavior (i.e. acoustic signaling from exposed perches) by being more reactive to potential threats