Opening remarks(Sustainable Yield and Population Conservation for Marine Organisms from the Point of View of Genetic Resources,International Workshop in Faculty of Agricultural Science and Field Science Center in Tohoku University 2008)

Original Pape

Introduction of Research Institutes for Aquatic and Marine Science in Miyagi Prefecture

In Miyagi Prefecture, there are four research institutes for aquatic field science. These institutes have been advanced basic and applied research for fisheries management system not only by themselves but also with Tohoku University. Here, I introduce these institutes briefly on behalf of them and discuss some future joint programs as alliance of Miyagi regional aquatic field science

Identification of Novel Microsatellite Loci in the Pacific Oyster (Crassostrea gigas) by Magnetic Bead Hybridization Selection

microsatellitePacific oystergenetic variabilit

Gynogenetic Tetraploid Larvae of the Pacific Oyster Crassostrea gigas Induced by Inhibition of the 1st and 2nd Meiotic Divisions

The induction of gynogenetic tetraploids by the suppression of the meiotic division in the Pacific oyster Crassostrea gigas was attempted in three trials using the treatments of cytochalasin B (0.5μg/mL; CB). The effects of various durations of CB treatment on tetraploid induction frequency were examined. Haploid gynogenesis was induced by sperm which were UV-irradiated for 60s at an intensity of 72erg/mm^2/s. CB treatment was effective in inhibiting meiosis, and the optimum time for the suppression of both the 1st and 2nd polar bodies was from 10 to 45min postinsemination, yielding 51.4% gynogenetic tetraploids. The results of this study suggest the possibility of producing gynogenetic tetraploid Pacific oysters