Constitutive expression of bergaptol O-methyltransferase in Glehnia littoralis cell cultures.

We investigated whether exogenously supplied precursors of bergapten, namely umbelliferone, psoralen and bergaptol, could be utilized to produce bergapten without elicitation in Glehnia littoralis cell suspension cultures. The levels of added psoralen and bergaptol in the medium soon decreased, and this was followed by the detection of bergapten in both culture fluid and cells. Umbelliferone was also incorporated but in this case no bergapten was produced; instead, skimmin, umbelliferone monoglucoside, was detected. To determine whether conversion of psoralen to bergapten was due to enzyme induction by precursor feeding, the transcript accumulations and enzyme activities of bergaptol O-methyltransferase (BMT, EC 2.1.1.69), which catalyzes the last step of bergapten synthesis, and of phenylalanine ammonia-lyase (PAL, EC 4.3.1.5), which catalyzes the initial step of the phenylpropanoid biosynthetic pathway and is known as a marker enzyme of elicitation, were examined. The results showed that both the expression and the activity of BMT were always detected in all cells, including control cells. Since PAL was slightly induced in the cells supplied with/without precursors, phenylethyl alcohol (PEA, a competitive inhibitor of PAL) was applied to suspension cells prior to the addition of psoralen. PAL activity was effectively inhibited by PEA at 1-5 mM concentrations. Under these conditions, PEA did not affect bergapten production by cell cultures fed with psoralen at all. These results demonstrate that BMT is constitutively expressed in G. littoralis cell cultures

Variation in size of living articulated brachiopods with latitude and depth

Geographical variations in animal characters are one of the main subjects for study in macroecology. Variation with latitude has received special interest. Articulated brachiopods are possibly the commonest macrofossil with large variations in size of taxa through the fossil record. Here, we investigate trends in size of the 3 main orders of articulated brachiopod with latitude and depth. Data were insufficient to identify patterns in Thecideida (a micromorph taxon only recorded from low latitudes). Rhynchonellida had no clear trends in size with latitude or depth. Terebratulida exhibited hemispheric differences in size relations, with increasing length of species towards the pole in the south and no significant trend in the north. Tropical species were small (< 20 mm length between 10A degrees N and 10A degrees S), and the largest species were found between 30A degrees and 60A degrees latitude in both hemispheres. There were no articulated brachiopods recorded from the high arctic, and support for a continuous trend in size with latitude was small or absent. In Terebratulida, there was a significant decrease in species length with depth of 1.7 mm per 100 m depth increase. These trends could be explained by competition for space and reduced availability of habitat with progressive depth beyond the continental shelf