44 research outputs found
In Vitro Cultures of Schisandra chinensis (Turcz.) Baill. (Chinese Magnolia Vine)—a Potential Biotechnological Rich Source of Therapeutically Important Phenolic Acids
The contents of free phenolic acids and cinnamic acid were determined using an HPLC method in methanolic extracts from biomass of Schisandra chinensis (Turcz.) Baill. (Chinese magnolia vine) at different stages of organogenesis, cultured in vitro on a few variants of Murashige and Skoog (MS) medium, containing different concentrations of plant growth regulators 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) (from 0.1 to 3.0Â mg/l) and in extracts from overground parts of plants growing in vivo. Six of 12 analysed compounds were detected in all extracts: chlorogenic, p-coumaric, p-hydroxybenzoic, protocatechuic, salicylic and syringic acids. Total contents of the examined metabolites in biomass of shoot-differentiating callus culture cultivated on six MS medium variants were dependent on concentrations of growth regulators in the media and ranged from 14.90 to 60.05Â mg/100Â g d.w. Total contents of the compounds in biomass extracts from undifferentiating callus culture maintained only on two of six MS medium variants were higher and amounted to 74.54 and 78.24Â mg/100Â g d.w. Maximum total contents of phenolic acids in both types of in vitro cultures were greater than in fruits (55.73Â mg/100Â g d.w.) and leaves (4.55Â mg/100Â g d.w.) of plants gowning in vivo. Chlorogenic acid and salicylic acid were the main compounds identified in biomass extracts of shoot-differentiating callus cultures (max 22.60 and 21.17Â mg/100Â g d.w., respectively), while chlorogenic acid (max 38.43Â mg/100Â g d.w.) and protocatechuic acid (max 20.95Â mg/100Â g d.w.) prevailed in the extracts from undifferentiating callus cultures. Other compounds dominated in fruits, namely p-coumaric acid (23.36Â mg/100Â g d.w.) and syringic acid (14.96Â mg/100Â g d.w.). This is the first report on biochemical potential of cells from S. chinensis in vitro cultures to produce the biologically active phenolic acids. These are the first results on the analysis of this group of metabolites in overground parts of plants growing in vivo, too
Unique natural fibers: physicochemical characteristics of lignin-cellulosic fibers from aechmea bromeliifolia leaves
Ligno-cellulosic fibers are abundantly present in a diverse flora. Bromeliaceae is considered one of the most prominent vegetable family in terms of the availability of these fibrous plants, making for excellent textile leaf fibers. The objective of this work is to present a preliminary physicochemical characterization of the Brazilian-native Aechmea Bromeliifolia fiber. The fibers were manually extracted from the species’ leaves to be studied in terms of their physical and chemical structure, and mechanical behavior. The results were compared to known fibers in the textile industry, identifying the fiber’s potential for textile application. Nevertheless, further study is needed for property optimization and the improvement of extraction and treatment methods.The authors gratefully acknowledge the funding by Support Foundation of
the Institute for Technological Research, FIPT, Brazil, under grants FLORATEXTIL I. “This
work is also supported by FEDER funds through the Competitivity Factors Operational Programme - COMPETE and by national funds through FCT – Foundation for Science and
Technology within the scope of the project POCI-01-0145-FEDER-007136”