The multifaceted use of Lactobacillus reuteri DSM 17938 in a pediatric clinic: a retrospective observational study

Background: Lactobacillus reuteri DSM 17938 is a well-studied probiotic which colonizes many mammals. In humans, L. reuteri is found in various body sites including the gastrointestinal tract, urinary tract, skin and breast milk. It fulfills all the prerequisites of being a good probiotic as described by the world gastroenterology organization.Methods: This was a retrospective observational study, where a nutritional supplement L. reuteri DSM 17938 was given to 197 children, aged 0-16 years, along with normal diet. The participants were having complains of diarrhea, stomach pain and frequent hospitalization. Various parameters were recorded such as sex, age, anthropometry-weight and height (every visit), diagnosis, reason for outpatient visits and various demography including but not limited to- birthplace, current location, year in school, reason for hospital admission (if any). Paired t test was used to find difference in weight and height between each visit.Results: A total of 118 subjects were included in the analysis. Five drops of nutritional supplement L. reuteri DSM 17938 was given. Supplementation of L. reuteri reduced the need for hospital admissions. 96% children did not require hospital admission. It improved the overall health of the children. There was a statistical significance in the height and weight between first and second visits (p<0.001). A 7.65% weight increase was noted in between first and second visit. A 2.32% height increase was seen between first and second visit.Conclusions: L. reuteri DSM 17938 given as a nutritional supplement improved the health of the children and reduced the need for hospital admissions.

ACUTE AND SUBACUTE TOXICITY STUDIES OF ETHANOLIC EXTRACT OF MIRABILIS JALAPA LINN IN WISTAR ALBINO RATS

Objective: The objective of the study was to evaluate the toxicological potential of the ethanolic extract of leaves of Mirabilis jalapa linn through acute and subacute toxicity studies in albino Wistar rats. Methods: For acute toxicity studies, the ethanolic extract of M. jalapa was given up to 2000 mg/kg and then the animals were observed for 14 days to find out any adverse effect or death. For sub-acute toxicity studies, the exact was given for 28 days and the following parameters were observed such as changes in body weight, food intake, water intake, hematological parameters, biochemical parameters, lipid profile, urine analysis, and histopathological studies were undertaken. Results: Single oral administration of 2000 mg/kg of the ethanolic extract of M. jalapa produced no mortality or signs of toxicity. During subacute toxicity there were no changes in body weight, food intake and water intake were observed. There were no changes in lipid profile, hematological parameters, and biochemical parameters. In histopathological changes, there were no structural changes in treated groups when compared to control. Conclusion: The leaves of ethanolic extract of M. jalapa is safe when administered for 28 days. There were no deaths or signs of toxicity in treated rats during acute toxicity studies and subacute toxicity studies

Role of <i>Lactobacillus reuteri</i> <i>DSM 17938</i> on Crying Time Reduction in Infantile Colic and Its Impact on Maternal Depression: A Real-Life Clinic-Based Study

Background: Infantile colic is characterized by prolonged periods of inconsolable, incessant crying and persistent fussing in an otherwise healthy infant. It is a self-limiting condition, but causes significant stress to mothers. AIM: To observe the role of Lactobacillus reuteriDSM 17938 in reducing crying time in colicky infants in routine clinical practice. Methods: This was a prospective observational multicentric clinic-based study. Each practitioner included approximately 30 infants L. reuteri DSM 17938 for a period of 21 days. There were four physical consultations and two telephonic consultations. The parents were given a daily diary to record the duration of crying and fussing episodes and a questionnaire was administered during the consultations. Results: A total of 120 infants with a mean age of 56.9 ± 34.2 days were included in this 28-day study. The mean crying time as reported by the parents in the subject diary reduced from 248.2 ± 101.2 min, 95% CI: 229.45, 266.94 at baseline to 45.6 ± 79.1 min 95% CI: 31.02, 60.31 at study end (P L. reuteri DSM 17938 was associated with a significant reduction in crying time in colicky infants, and showed improvement in maternal depression

Recommendations for the adjuvant use of the poly-antibiotic–resistant probiotic Bacillus clausii (O/C, SIN, N/R, T) in acute, chronic, and antibiotic-associated diarrhea in children: consensus from Asian experts

© 2020, The Author(s). This paper proposes recommendations for probiotics in pediatric gastrointestinal diseases in the Asia-Pacific region. Evidence-based recommendations and randomized controlled trials in the region are included. Cultural aspects, health management issues and economic factors were also considered. Final recommendations were approved by utilizing a modified Delphi process and applying the Likert scale in an electronic voting process. Bacillus clausii was recommended as an adjunct treatment with oral rehydration solution for acute viral diarrhea. B. clausii may also be considered for prevention of antibiotic-associated diarrhea, Clostridium difficile-induced diarrhea, and as adjunct treatment of Helicobacter pylori. There is insufficient evidence for recommendations in other conditions. Despite a diversity of epidemiological, socioeconomical and health system conditions, similar recommendations currently apply to most Asia-Pacific countries. Ideally, these need to be validated with local randomized-controlled trials