In vitro cytotoxicity of all-ceramic substructural materials after aging

Periodontitis oral bakterilerin sebep olduğu dişi &ccedil;evreleyen destek kemik ve yumuşak dokuları etkileyen kronik enflamatuar bir hastalıktır. Bu kronik enflamatuar s&uuml;recin sonunda destek dokudaki harabiyet diş kaybına kadar giden sorunlara yol a&ccedil;abilmektedir. Patojen mikroorganizmaların n&ouml;tralizasyonu i&ccedil;in l&ouml;kositlerce reaktif oksijen t&uuml;rlerinin aşırı salınması kronik periodontitisin konak kaynaklı yıkıma sebep olabilecek patolojik &ouml;zelliklerindendir. Şarap, &uuml;z&uuml;m ve &uuml;z&uuml;m &ccedil;ekirdeği ekstratı monomerik flavanol, kateşin, epikateşin ve oligomerik proantosiyanit gibi antioksidan olan polifenolik bileşenlerin ana kaynağıdır. Bu &ccedil;alışmanın amacı ratlarda ligat&uuml;rle ind&uuml;klenen periodontitiste &uuml;z&uuml;m &ccedil;ekirdeği ekstratının alveoler kemik yıkım seviyesi ile plazma total antioksidan durum, total oksidan durum ve osteokalsin seviyelerine etkisini değerlendirmektir. Bu sayede periodontitise bağlı olarak ger&ccedil;ekleşen, reaktif oksijen t&uuml;rlerindeki artışın etkilenme derecesi, ratlara &uuml;z&uuml;m &ccedil;ekirdeği verilerek, antioksidan &ouml;zelliğin alveol kemikteki yıkımla ilişkisi incelenecektir. &Ccedil;alışma Erciyes &Uuml;niversitesi Tıp Fak&uuml;ltesi Hakan &Ccedil;etinsaya Deneysel ve Klinik Araştırma Merkezinde(DEKAM) ger&ccedil;ekleştirilecektir. DEKAM?dan temin edilecek erkek Sprague-Dawley ratlar 4 gruba ayrılacaktır. 1. grubu ligat&uuml;rle ind&uuml;klenen periodontitis + 100 mg/kg &uuml;z&uuml;m &ccedil;ekirdeği gavaj 2. grubu ligat&uuml;rle ind&uuml;klenen periodontitis + 50 mg/kg &uuml;z&uuml;m &ccedil;ekirdeği gavaj 3. grubu &uuml;z&uuml;m &ccedil;ekirdeği verilmeksızın ligat&uuml;rle ind&uuml;klenen periodontitis 4. grubu kontrol ratları oluşturacaklardır. &Ccedil;alışmanın 14 g&uuml;n s&uuml;resinde uygulama kısımının tamamlanması planlanmaktadır. Hayvanlar uyutulup 1., 2. ve 3. grubun maksiller 1. molar dişlerine 5.0 ipek s&uuml;tur bağlanarak periodontitis ind&uuml;klenecektir. 1. gruba 14 g&uuml;n boyunca her g&uuml;n 100 mg/kg &uuml;z&uuml;m &ccedil;ekirdeği gavaj, 2. gruba ise 14 g&uuml;n boyunca 50 mg/kg &uuml;z&uuml;m &ccedil;ekirdeği gavaj yapılacaktır. 14. g&uuml;n sonunda t&uuml;m hayvanlardan plazma total antioksidan durum, total oksidan durum ve osteokalsin seviyelerindeki değişimin takibi i&ccedil;in kan &ouml;rnekleri alınıp hayvanlar kurban edilecektir. Alınan plazma &ouml;rneklerinin biyokimyasal analizi hizmet satın alımı ile &ouml;zel bir firmaya yaptırılacaktır.Hayvanların &uuml;st &ccedil;enesi &ccedil;ıkarılıp stereo mikroskop altında maksiller 1. ve 2. molar dişlerin mine-sement hududu alveol kemik kenarı arası mesafe &ouml;l&ccedil;&uuml;mleri ger&ccedil;ekleştirilecektir.&nbsp;In this study, we evaluated the effects of two different regimens of dietary supplementation with grape seed extract (GSE)based on serum total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) and osteocalcin (OC) inexperimental periodontitis. The investigation was performed at the Department of Animal Experimentation at Erciyes University,Kayseri, Turkey, from May 2011 to June 2011. Experimental periodontitis was induced by placing 5.0 silk sutures around themaxillary first molars. Twenty-seven adult male wistar rats were divided into four study groups as follows: Healthy control(HC; N = 7); Ligature only (LO; N = 6); Ligature-induced periodontitis plus GSE 50 mg/kg (GSE 50; N = 8); Ligature-inducedperiodontitis plus GSE 100 mg/kg (GSE 100; N = 6). GSE administration was performed for 14 days following induction ofexperimental periodontitis. On day 15, serum samples were obtained and rats were sacrificed. Serum samples were analyzed forTAS, TOS, OSI and OC. Defleshed jaws were analyzed morphometrically for alveolar bone loss. The results showed that placing5.0 silk sutures around maxillary first molars resulted in statistically significant bone loss compared to the HC group (P &lt; 0.05).None of the GSE administrated groups showed evidence that GSE was effective in preventing ligature-induced alveolar boneloss. The GSE 100 and GSE 50 groups had a significantly higher TAS compared to the HC group. No significant differences wereseen in TOS, OSI and OC levels. As a whole, GSE administration does not seem to influence TAS, TOS and OC. The lack of atherapeutic benefit of GSE in this study is difficult to explain, and further studies are required to fully assess the potential role ofGSE in periodontal treatment.</div

Effect of Low-Dose Doxycycline on Serum Oxidative Status, Gingival Antioxidant Levels, and Alveolar Bone Loss in Experimental Periodontitis in Rats

Background: Subantibiotic doses of doxycycline (lowdose doxycycline [LDD]) have been widely used in periodontal treatment for enzymatic inhibition and related anti-inflammatory properties. The aim of the present study is to verify the possible effects of LDD on oxidative stress in relation to periodontal attachment loss associated with ligature-induced experimental periodontal disease in rats.Background: Subantibiotic doses of doxycycline (lowdose doxycycline [LDD]) have been widely used in periodontal treatment for enzymatic inhibition and related anti-inflammatory properties. The aim of the present study is to verify the possible effects of LDD on oxidative stress in relation to periodontal attachment loss associated with ligature-induced experimental periodontal disease in rats.&nbsp;Methods: Thirty female Wistar albino rats were divided into three study groups as follows: 1) control (C) rats; 2) rats with experimental periodontitis (PED); and 3) rats with PED that were treated with doxycycline (PED + LDD). PED was induced by placing ligatures around the cervix of the maxillary second molars for 21 days. The PED + LDD group was treated orally with doxycycline (6 mg/kg) for 21 days after the ligature was placed. After 21 days, the rats were euthanized, and samples of the right maxilla were defleshed and used for histologic and morphometric analyses. The gingival tissue of the left maxilla was used for the analysis of lipid peroxidation (malondialdehyde [MDA]) and antioxidant enzymes (catalase, glutathione peroxidase, and superoxide dismutase). Levels of serum total antioxidant status (TAS)/total oxidant status (TOS) and oxidative stress index (OSI) were also analyzed.&nbsp;Results: Alveolar bone loss was significantly higher in the PED group compared with the PED + LDD and C groups (P &lt; 0.05). Doxycycline exhibited the most prominent inhibition on gingival tissue levels of MDA and antioxidant enzymes (P &lt; 0.05). Doxycycline also significantly reduced TOS and OSI levels (P &lt; 0.05) but increased the TAS level.&nbsp;Conclusion: Doxycycline helps to prevent periodontal tissue breakdown by inhibiting local and systemic oxidative stress.</p

A case of Mal de Meleda treated with acitretin

Mal de Meleda is a rare autosomal recessive skin disor-der characterized by palmoplantar keratoderma with 'glove and sock' distribution, keratotic skin lesions, pe-rioral erythema, brachydactyly and nail abnormalities. The efficacy of the oral retinoids in disorders of keratini-zation is well established. Herein, we present a 69 years old patient complainig about thickening of the skin of his palms and soles and diagnosed as Mal de Meleda and treated with acitretin

The relationship between periodontal disease severity and state-trait anxiety level

Objective: To determine the relationship between the paroxysm related to periodontal disease and the state-trait anxiety level in patients attending a periodontology clinic in Turkey

EFFECT OF SMOKING ON ATTACHMENT OF HUMAN PERIODONTAL LIGAMENT CELLS TO PERIODONTALLY INVOLVED ROOT SURFACES FOLLOWING ENAMEL MATRIX DERIVATIVE APPLICATION

The most important aim in periodontal therapy is to regenerate the periodontal supporting tissue lost as a result of periodontitis. Several studies have demonstrated that tobacco use interferes with periodontal therapy and substantially reduces the possibility of favorable treatment outcomes. The present study was performed to determine the attachment of PDL cells to the diseased roots of a smoking patient compared to non-smoking controls with enamel matrix derivative (EMD) application. Teeth both from a patient smoking more than 20 cigarettes daily and from another non-smoking patient were extracted and PDL tissue biopsies were taken from these teeth. Fibroblasts were cultured. Each root surface was divided into six equal parts. Samples were treated with citric acid and EMD, embedded into cell culture flasks, and kept in the culture for 1 h, 3 h, 5 h and 3 days. Then, electron microscopy analysis was performed. In the smoking group, collagen fibers were spread parallel to the surface as in the non-smoking group, but in one single direction rather than in different directions. It was observed that EMD application on smoking and non-smoking periodontally-diseased patients could affect the function of PDL cells and the potential of collagen production