Relationship between serum sialic acid levels and eye lesions in calves with infectious bovine keratoconjunctivitis

The aim of this study was to evaluate the clinical and biochemical importance of sialic acid (SA) serum concentrations (total, lipid-bound and protein-bound) in calves suffering from infectious bovine keratoconjunctivitis (IBK). The study included 18 calves with IBK (Group 1) and I I healthy calves (Group 11). Blood samples were collected from all calves for the determination of serum SA and glucose concentrations. Eye lesions were scored as mild, moderate and severe based on severity of the clinical symptoms. Mean total sialic acid (TSA), lipid-bound sialic acid (LBSA) and protein-bound sialic acid (PBSA) concentrations of the calves with IBK were 106,9 +/- 3,6 mg/dl, 54,81 +/- 2,78 mg/dl, 52,1 +/- 3,1 mg/dl, respectively. Values of TSA, LBSA and PBSA determined for healthy calves were 76,4 +/- 3,1 mg/dl, 38,4 +/- 2,1 mg/dl and 38 +/- 3,7 mg/dl, respectively. Serum TSA and LBSA values measured in animals from group I were significantly higher than those of animals from group 11 (p<0.001). As the severity of eye lesions increased, the LBSA serum concentration was also increased reaching 47.5 +/- 1.8 mg/dl; 50.8 +/- 0.9 mg/dl; 66.1 +/- 6.1 mg/dl for calves presenting mild, moderate and severe lesions, respectively

Evaluation of serum sialic acid as an inflammation marker in cattle with traumatic reticulo peritonitis

The present study was designed to determine the diagnostic value of serum sialic acid (SA) concentration in cattle with traumatic reticulo-peritonitis (TRP). The study involved 12 cattle with TRP and 12 healthy cattle. Blood samples were collected from cattle for the determination of serum sialic acid, total protein, albumin, globulin concentrations, gluteraldehyde test and total leukocyte count. Mean concentration of total sialic acid (TSA), lipid bound sialic acid (LBSA) and protein bound sialic acid (PBSA) were 1.163 +/- 0.123 g/l 0.615 +/- 0.112 g/l and 0.548 +/- 0.120 g/l for the cattle wit TRP and 0.761 +/- 0.152 g/l, 0.333 +/- 0.102 g/l and 0.428 +/- 0.144 g/l for clinically healthy cattle, respectively. Serum TSA and LBSA concentrations in cattle with TRP were statistically higher (p<0.00 1) than that of healthy cattle. Increases in serum SA values were in parallel with the other inflammation markers (total protein, globulin, glutaraldheyde test, WBC) examined in this study. With a threshold of decision calculated by mean value + 1 standard deviation of control group, sensitivity of TSA and LBSA test were 100% and specificity were 91.7% and 75% respectively

Serum sialic acid and oxidative stress parameters changes in cattle with leptospirosis

This study was designed to disclose some indicators of oxidative stress and inflammation in natural cases of bovine leptospirosis. For this purpose, 12 bulls exhibiting clinical signs of leptospirosis and 10 healthy bulls were used. Animals were subjected to thorough clinical examination and the clinical signs were recorded. All animals were blood sampled in order to determine serum total sialic acid (TSA), lipid bound sialic acid (LBSA), malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), uric acid (UA), total protein (TP), albumin and glucose. Urine samples were collected from each animal and examined under dark-field microscope to observe spirochetes. Diseased animals exhibited clinical signs suggesting leptospirosis and the diagnosis was supported by positive dark-field microscope examination. Mean TSA (mmol/L), LBSA (mmol/L), TP (g/dl), albumin (g/dl), glucose (mg/dl), MDA (mu mol/L), GSH (mg/dl), NO (nmol/ml), and UA (mg/L) levels were 1.63 +/- 0.02, 0.40 +/- 0.10, 7.18 +/- 0.24, 3.23 +/- 0.5, 64.96 +/- 1.88, 5.71 +/- 0.11, 78.68 +/- 0.72, 7.94 +/- 0.34, and 8.75 +/- 0.41 in healthy bulls, and 2.50 +/- 0.05, 0.70 +/- 0.2, 9.27 +/- 0.17, 2.55 +/- 0.62, 107.93 +/- 2.52, 8.82 +/- 0.14, 47.85 +/- 1.85, 14.57 +/- 0.63 and 15.85 +/- 0.80 in leptospirosis cases, respectively. The differences between the two groups were statistically significant (P < 0.001). Increased TSA, LBSA, MDA, NO, UA, TP, glucose and decreased GSH and albumin concentrations were suggestive of inflammation and oxidative stress in diseased bulls. The results obtained may suggest that oxidative damage along with other mechanisms might have taken part in the pathogenesis of bovine leptospirosis and further detailed studies are needed to fully understand the mechanism(s) of the disease