Empowering communities through participatory monitoring and evaluation in Tororo district

The Community Based Participatory Monitoring and Evaluation (CB-PME) tool empowers poor local farming communities to improve their livelihoods. While this process is people centred, it draws on local people’s capacities, while giving the end users of a technology a voice. The experience of the Katamata farmers’ group in Tororo district using PM&E is given in this paper. This group have embarked on a commercial groundnut production enterprise to improve their livelihoods. They decided to monitor one and three year-prioritized objectives for this enterprise. The three year objectives were based on the improvement of the farmers’ livelihoods and included having food security, with a marketed surplus, creating an awareness of HIV/AIDS, and individual commercial production, while the one year objectives dealt with the improvement in agricultural production. Some short term objectives have been achieved. The farmers have selected the best variety for commercial groundnut production. They have since reflected on these objectives and indicators with the monitoring and evaluation committee from their group whose main function is to collect, synthesize, store and report information to the group, community and visitors. This participatory process has empowered farmers to make production and marketing decisions on their enterprises and social lives with the ultimate goal of improving their livelihoods

Unlocking new alleles for leaf rust resistance in the Vavilov wheat collection

Thirteen potentially new leaf rust resistance loci were identified in a Vavilov wheat diversity panel. We demonstrated the potential of allele stacking to strengthen resistance against this important pathogen. Leaf rust (LR) caused by Puccinia triticina is an important disease of wheat (Triticum aestivum L.), and the deployment of genetically resistant cultivars is the most viable strategy to minimise yield losses. In this study, we evaluated a diversity panel of 295 bread wheat accessions from the N. I. Vavilov Institute of Plant Genetic Resources (St Petersburg, Russia) for LR resistance and performed genome-wide association studies (GWAS) using 10,748 polymorphic DArT-seq markers. The diversity panel was evaluated at seedling and adult plant growth stages using three P. triticina pathotypes prevalent in Australia. GWAS was applied to 11 phenotypic data sets which identified a total of 52 significant marker-trait associations representing 31 quantitative trait loci (QTL). Among them, 29 QTL were associated with adult plant resistance (APR). Of the 31 QTL, 13 were considered potentially new loci, whereas 4 co-located with previously catalogued Lr genes and 14 aligned to regions reported in other GWAS and genomic prediction studies. One seedling LR resistance QTL located on chromosome 3A showed pronounced levels of linkage disequilibrium among markers (r (2)\ua0=\ua00.7), suggested a high allelic fixation. Subsequent haplotype analysis for this region found seven haplotype variants, of which two were strongly associated with LR resistance at seedling stage. Similarly, analysis of an APR QTL on chromosome 7B revealed 22 variants, of which 4 were associated with resistance at\ua0the adult plant stage. Furthermore, most of the tested lines in the diversity panel carried 10 or more combined resistance-associated marker alleles, highlighting the potential of allele stacking for long-lasting resistance