11 research outputs found
Coherent quantum phase slip
A hundred years after discovery of superconductivity, one fundamental
prediction of the theory, the coherent quantum phase slip (CQPS), has not been
observed. CQPS is a phenomenon exactly dual to the Josephson effect: whilst the
latter is a coherent transfer of charges between superconducting contacts, the
former is a coherent transfer of vortices or fluxes across a superconducting
wire. In contrast to previously reported observations of incoherent phase slip,
the CQPS has been only a subject of theoretical study. Its experimental
demonstration is made difficult by quasiparticle dissipation due to gapless
excitations in nanowires or in vortex cores. This difficulty might be overcome
by using certain strongly disordered superconductors in the vicinity of the
superconductor-insulator transition (SIT). Here we report the first direct
observation of the CQPS in a strongly disordered indium-oxide (InOx)
superconducting wire inserted in a loop, which is manifested by the
superposition of the quantum states with different number of fluxes. Similarly
to the Josephson effect, our observation is expected to lead to novel
applications in superconducting electronics and quantum metrology.Comment: 14 pages, 3 figure
Trim17, novel E3 ubiquitin-ligase, initiates neuronal apoptosis
Accumulating data indicate that the ubiquitin-proteasome system controls apoptosis by regulating the level and the function of key regulatory proteins. In this study, we identified Trim17, a member of the TRIM/RBCC protein family, as one of the critical E3 ubiquitin ligases involved in the control of neuronal apoptosis upstream of mitochondria. We show that expression of Trim17 is increased both at the mRNA and protein level in several in vitro models of transcription-dependent neuronal apoptosis. Expression of Trim17 is controlled by the PI3K/Akt/GSK3 pathway in cerebellar granule neurons (CGN). Moreover, the Trim17 protein is expressed in vivo, in apoptotic neurons that naturally die during post-natal cerebellar development. Overexpression of active Trim17 in primary CGN was sufficient to induce the intrinsic pathway of apoptosis in survival conditions. This pro-apoptotic effect was abolished in Bax(-/-) neurons and depended on the E3 activity of Trim17 conferred by its RING domain. Furthermore, knock-down of endogenous Trim17 and overexpression of dominant-negative mutants of Trim17 blocked trophic factor withdrawal-induced apoptosis both in CGN and in sympathetic neurons. Collectively, our data are the first to assign a cellular function to Trim17 by showing that its E3 activity is both necessary and sufficient for the initiation of neuronal apoptosis. Cell Death and Differentiation (2010) 17, 1928-1941; doi: 10.1038/cdd.2010.73; published online 18 June 201