Sex differences in the effect of chronic delivery of the buprenorphine analogue BU08028 on heroin relapse and choice in a rat model of opioid maintenance

Background and Purpose: Maintenance treatment with opioid agonists (buprenorphine, methadone) decreases opioid use and relapse. We recently modelled maintenance treatment in rats and found that chronic delivery of buprenorphine or the μ opioid receptor partial agonist TRV130 decreased relapse to oxycodone seeking and taking. Here, we tested the buprenorphine analogue BU08028 on different heroin relapse-related measures and heroin versus food choice. Experimental Approach: For relapse assessment, we trained male and female rats to self-administer heroin (6 h·day−1, 14 days) in Context A and then implanted osmotic minipumps containing BU08028 (0, 0.03 or 0.1 mg·kg−1·d−1). Effects of chronic BU08028 delivery were tested on (1) incubation of heroin-seeking in a non-drug Context B, (2) extinction responding reinforced by heroin-associated discrete cues in Context B, (3) reinstatement of heroin-seeking induced by re-exposure to Context A and (4) re-acquisition of heroin self-administration in Context A. For choice assessment, we tested the effect of chronic BU08028 delivery on heroin versus food choice. Key Results: Chronic BU08028 delivery decreased incubation of heroin seeking. Unexpectedly, BU08028 increased re-acquisition of heroin self-administration selectively in females. Chronic BU08028 had minimal effects on context-induced reinstatement and heroin versus food choice in both sexes. Finally, exploratory post hoc analyses suggest that BU08028 decreased extinction responding selectively in males. Conclusions and Implications: Chronic BU08028 delivery had both beneficial and detrimental, sex-dependent, effects on different triggers of heroin relapse and minimal effects on heroin choice in both sexes. Results suggest that BU08028 would not be an effective opioid maintenance treatment in humans.</p

+21dBm erbium power amplifier pumped by a diode-pumped Nd:YAG laser

Efficient energy transfer has been demonstrated in an Er/Yb co-doped phosphorus doped silica fiber for the first time. This has indirectly allowed the use of reliable, high-power AlGaAs diode laser arrays as the semiconductor pump source through the use of a diode-pumped Nd:YAG (DPL) laser operating at 1064 nm. Small signal gains of 42 dB and output powers of 71 mW (+18.5 dBm) have been observed with a single DPL. Bidirectional pumping with two DPLs has yielded an output power of 130 mW (+21 dBm)

Yb³⁺ sensitised Er³⁺-doped silica optical fibre with ultrahigh transfer efficiency and gain

Efficient 1.54µm emission under 1064nm excitation of Er3+:Yb3+ codoped silica fibre is reported. The energy transfer efficiency from Yb3+ to Er3+, ~95% even under high inversion, is comparable to that in multicomponent glass fibres. The small signal gain of ~45dB is measured and power amplifiers with 145mW output power demonstrated

High-gain fibre power amplifier tandem-pumped by a 3W multi-stripe diode

An Nd3+-doped compound-glass fibre laser pumped by a 3W AlGaAs laser diode array is employed as pump source for an Er3+/Yb3+ optical fibre power amplifier. An amplifier gain of 45dB and output signal powers in excess of +20dBm are demonstrated

+20dBm erbium power amplifier pumped by a diode-pumped Nd:YAG laser

Efficient energy transfer has been demonstrated in an Er/Yb co-doped phosphorus doped silica fiber for the first time. This has indirectly allowed the use of reliabIe, high power AlGaAs diode laser arrays as the semiconductor pump source through the use of a diode-pumped Nd:YAG (DPL) laser operating at 1064 nm. Small signal gains of 40 dB and output powers of 69 mW (+18.4 dBm) have been observed with a single DPL Bi-directional pumping with two DPL's has yielded an output power of 145 mW (+21.5dBm)

Studies on the presence and persistence of Pasteurella multocida serovars and genotypes in fowl cholera outbreaks

Pasteurella multocida was isolated from poultry on six farms (one free-range duck farm, one free-range turkey farm, one conventional enclosed turkey farm, and three free-range layer farms) suffering fowl cholera outbreaks. In addition, historical isolates from previous outbreaks were available for the conventional turkey farm and the three free-range layer farms. The isolates were serotyped using the Heddleston scheme and genotyped using multi-locus sequencing typing. In the current outbreaks, two of the farms had two different sequence types (STs) of P. multocida in the investigated outbreak (the free-range turkey farm and one of the free-range layer farms). The remaining four farms had one ST within the investigated outbreak. In looking at the historical isolates, two of the four farms had multiple genotypes involved. On the four farms with historical isolates from previous outbreaks, at least one new genotype was present in the investigated outbreak as compared with the historical isolates. On one layer farm, one genotype persisted over a 10-year period. Serotyping revealed the presence of multiple serovars in the current and historical outbreaks, with serovars sometimes changing over time. This study has shown that several STs of P. multocida can be present during some outbreaks of fowl cholera, although other outbreaks involve a single ST. Also, the STs present on a property suffering repeated fowl cholera can both persist and change over time. © 201