The Structure of a Rigorously Conserved RNA Element within the SARS Virus Genome

We have solved the three-dimensional crystal structure of the stem-loop II motif (s2m) RNA element of the SARS virus genome to 2.7-Å resolution. SARS and related coronaviruses and astroviruses all possess a motif at the 3′ end of their RNA genomes, called the s2m, whose pathogenic importance is inferred from its rigorous sequence conservation in an otherwise rapidly mutable RNA genome. We find that this extreme conservation is clearly explained by the requirement to form a highly structured RNA whose unique tertiary structure includes a sharp 90° kink of the helix axis and several novel longer-range tertiary interactions. The tertiary base interactions create a tunnel that runs perpendicular to the main helical axis whose interior is negatively charged and binds two magnesium ions. These unusual features likely form interaction surfaces with conserved host cell components or other reactive sites required for virus function. Based on its conservation in viral pathogen genomes and its absence in the human genome, we suggest that these unusual structural features in the s2m RNA element are attractive targets for the design of anti-viral therapeutic agents. Structural genomics has sought to deduce protein function based on three-dimensional homology. Here we have extended this approach to RNA by proposing potential functions for a rigorously conserved set of RNA tertiary structural interactions that occur within the SARS RNA genome itself. Based on tertiary structural comparisons, we propose the s2m RNA binds one or more proteins possessing an oligomer-binding-like fold, and we suggest a possible mechanism for SARS viral RNA hijacking of host protein synthesis, both based upon observed s2m RNA macromolecular mimicry of a relevant ribosomal RNA fold

The Plasma Environment of Comets

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/138863/1/rog199129s2976.pd

Intercomparison of various measurements of thermal plasma densities at and near the plasmapause

Four methods of investigating the thermal plasma density near the plasmapause have been intercompared for the period 1 to 15 July 1972. These methods are whistlers, the double floating probe on Explorer 45, three IMP I plasma wave signatures and observations made aboard both Prognoz 1 and Prognoz 2. Explorer 45 data have provided new information on the plasmapause bulge which, during this period, occurs at 16 L.T. This displacement from the accepted time of 18 L.T. or even later is substantiated by the Russian satellites. All methods give the result that the plasmapause is found at an electron number density somewhere between 20 and 120 cm−3 or, alternatively, at 60 cm−3, to within a factor of 2