4,240 research outputs found
Four-Parameter white blood cell differential counting based on light scattering measurements
Measurement of the depolarized orthogonal light scattering in flow cytometry enables one to discriminate human eosinephilic granulocytes from neutrophilic granulocytes. We use this method to perform a four-parameter differential white blood cell analysis. \ud
A simple flow cytometer was built equipped with a 5-mW helium neon laser that measures simultaneously four light scattering parameters. Lymphocytes, monocytes, and granulocytes were identified by simultaneously measuring the light scattering intensity at angles between 1.0° and 2.6° and angles between 3.0° and 11.0°. Eosinophilic granulocytes were distinguished from neutrophilic granulocytes by simultaneous measurement of the orthogonal and depolarized orthogonal light scattering. \ud
Comparison of a white blood cell differentiation of 45 donors obtained by the Technicon H-6000 and our instrument revealed good correlations. The correlation coefficients (r2) found were: 0.99 for lymphocytes, 0.76 for monocytes, 0.99 for neutrophilic granulocytes, and 0.98 for eosinophilic granulocytes. The results demonstrate that reliable white blood cell differentiation of the four most clinically relevant leukocytes can be obtained by measurement of light scattering properties of unstained leukocytes
Relativistic general-order coupled-cluster method for high-precision calculations: Application to Al+ atomic clock
We report the implementation of a general-order relativistic coupled-cluster
method for performing high-precision calculations of atomic and molecular
properties. As a first application, the static dipole polarizabilities of the
ground and first excited states of Al+ have been determined to precisely
estimate the uncertainty associated with the BBR shift of its clock frequency
measurement. The obtained relative BBR shift is -3.66+-0.44 for the 3s^2
^1S_0^0 --> 3s3p ^3P_0^0 transition in Al+ in contrast to the value obtained in
the latest clock frequency measurement, -9+-3 [Phys. Rev. Lett. 104, 070802
(2010)]. The method developed in the present work can be employed to study a
variety of subtle effects such as fundamental symmetry violations in atoms.Comment: 4 pages, 3 tables, submitte
A Comparison of Near-Infrared Photometry and Spectra for Y Dwarfs with a New Generation of Cool Cloudy Models
We present YJHK photometry, or a subset, for the six Y dwarfs discovered in
WISE data by Cushing et al.. The data were obtained using NIRI on the Gemini
North telescope. We also present a far-red spectrum obtained using GMOS-North
for WISEPC J205628.90+145953.3. We compare the data to Morley et al. (2012)
models, which include cloud decks of sulfide and chloride condensates. We find
that the models with these previously neglected clouds can reproduce the energy
distributions of T9 to Y0 dwarfs quite well, other than near 5um where the
models are too bright. This is thought to be because the models do not include
departures from chemical equilibrium caused by vertical mixing, which would
enhance the abundance of CO, decreasing the flux at 5um. Vertical mixing also
decreases the abundance of NH_3, which would otherwise have strong absorption
features at 1.03um and 1.52um that are not seen in the Y0 WISEPC
J205628.90+145953.3. We find that the five Y0 to Y0.5 dwarfs have 300 < T_eff K
< 450, 4.0 < log g < 4.5 and f_sed ~ 3. These temperatures and gravities imply
a mass range of 5 - 15 M_Jupiter and ages around 5 Gyr. We suggest that WISEP
J182831.08+265037.8 is a binary system, as this better explains its luminosity
and color. We find that the data can be made consistent with observed trends,
and generally consistent with the models, if the system is composed of a T_eff
= 325 K and log g ~ 4.0
secondary, corresponding to masses of 10 and 7 M_Jupiter and an age around 2
Gyr. If our deconvolution is correct, then the T_eff = 300 K cloud-free model
fluxes at K and W2 are too faint by 0.5 - 1.0 magnitudes. We will address this
discrepancy in our next generation of models, which will incorporate water
clouds and mixing.Comment: 39 pages, 10 Figures, 8 Tables. Accepted by ApJ. This revision
replaces Figures 9 and 10 with B & W versions, corrects figure captions for
color online only, corrects references. Text is unchanged. Tables 3, 4 and 8
are available at http://www.gemini.edu/staff/sleggett, other model data are
available at http://www.ucolick.org/~cmorley/cmorley/Data.htm
High temperature condensate clouds in super-hot Jupiter atmospheres
Deciphering the role of clouds is central to our understanding of exoplanet
atmospheres, as they have a direct impact on the temperature and pressure
structure, and observational properties of the planet. Super-hot Jupiters
occupy a temperature regime similar to low mass M-dwarfs, where minimal cloud
condensation is expected. However, observations of exoplanets such as WASP-12b
(Teq ~ 2500 K) result in a transmission spectrum indicative of a cloudy
atmosphere. We re-examine the temperature and pressure space occupied by these
super-hot Jupiter atmospheres, to explore the role of the initial Al- and
Ti-bearing condensates as the main source of cloud material. Due to the high
temperatures a majority of the more common refractory material is not depleted
into deeper layers and would remain in the vapor phase. The lack of depletion
into deeper layers means that these materials with relatively low cloud masses
can become significant absorbers in the upper atmosphere. We provide
condensation curves for the initial Al- and Ti-bearing condensates that may be
used to provide quantitative estimates of the effect of metallicity on cloud
masses, as planets with metal-rich hosts potentially form more opaque clouds
because more mass is available for condensation. Increased metallicity also
pushes the point of condensation to hotter, deeper layers in the planetary
atmosphere further increasing the density of the cloud. We suggest that planets
around metal-rich hosts are more likely to have thick refractory clouds, and
discuss the implication on the observed spectra of WASP-12b.Comment: Accepted for publication in MNRAS, 10 pages, 1 table, 5 figure
Multi-, inter- and transdisciplinarity in challenge-based engineering education
Challenge-based learning (CBL) offers students in engineering programmes an opportunity to develop communicative and collaborative skills, apply disciplinary knowledge and develop boundary-crossing competencies. Mono-disciplinary approaches to CBL are generally regarded too limited, but whether multi-disciplinary, interdisciplinary, or transdisciplinary approaches should be used is open to discussion. Often, these concepts are used interchangeably, but there are notable differences. In literature, knowledge integration is mostly mentioned to make a distinction, but because of difficulties in applying this concept to education, we focus on tangible differences in educational practices, related to learning objectives, assessment, and the design of challenges. The different forms of CBL are illustrated by three case-studies carried out at a research university in the Netherlands. We found similarities, but also some subtle differences between multi-, inter- and transdisciplinary approaches to CBL. Multidisciplinary CBL projects are relatively pre-structured, with an indication of the knowledge that is to be applied, deepened, or combined. Interdisciplinary CBL is more open-ended, with students made responsible for connecting their disciplinary backgrounds to the project and for integrating disciplinary perspectives. Transdisciplinary CBL focuses more on impact than on integrating disciplinary contributions. Challenges are open-ended from a content and stakeholder perspective, while structure emerges in the interactions between students, teachers and stakeholders. Which form of CBL can best be employed in a course or programme is dependent on the intended learning objectives. Educators should be aware of tradeoffs and of the specific teacher competences required to design and support these different forms of CBL.</p
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