Introduction of Maclura Pomifera (RAF.) C.K. Schneid, Moraceae Link families under the conditions of the botanical graden of the NRU “Belsu” (Belgorod, Russia)

The data of long-term studies of the biological features of growth and development of Maclurapomifera (Raf.) C. K. Schneid in the Botanical Garden of the National Research University “BelSU” is given. The phenological type of development of this species, the beginning and end of its growing season is determined. Winter hardiness, drought resistance, resistance to diseases and pests are assessed to determine the degree of adaptation of the species to local conditions. For the first time, flowering and fruiting of Maclurapomifera were noted in the conditions of the Belgorod region. Based on the adaptation coefficient, recommendations are given on the use of Maclurapomifera in gardening in the Belgorod Regio

Analysis of rare type of plants from botanical graden collection at SRU “Belsu” (Belgorod, Russia)

The aim of the study was the comprehensive analysis of rare plants from the collection fund of the botanical garden at the National Research University “BelSU

Collection of cover-ground plants and plants for Alpinarians and Rocarians in botanical garden of Belgorod University (Belgorod, Russia)

The authors studied the introduced herbaceous perennials, that were not used previously for landscaping of the region. According to the data presented in the article, recommendations can be given for gardening in various landscape areas, proceeding from the decorative and ecological properties of plants. They identified the perennial ground cover plants unpretentious to the conditions of the urban environment, which can be used in sunny areas, in shades and half-shade

The attitude of the students of USMU is conducted to flu vaccination.

The article describes the results of a survey of students of USMU of different courses and faculties in relation to the effectiveness of influenza vaccination and their commitment to the method of specific prevention.В статье рассмотрены результаты анкетирования студентов УГМУ разных курсов и факультетов в отношении эффективности вакцинации против гриппа и их приверженность к проведению метода специфической профилактики

Synthesis of Carboxymethyl Starch for increasing drilling mud quality in drilling oil and gas wells

This paper describes the impact of carboxymethyl starch preparation conditions on physicochemical properties of polysaccharide reagent, widely used as fluid loss reducing agent in drilling mud. Variation of the main parameters of carboxymethylation is researched in the experiment. The following conditions such as temperature and reaction time, amount of water, as well as ratio of NaOH to monochloracetic acid define the characteristics of carboxymethyl starch. The degree of substitution is defined for polysaccharides, as well as the characteristics of samples have been studied by infrared spectroscopy. Rheological characteristics and fluid loss indicator have been investigated to study the impact of the reagents on drilling mud quality

Previously unidentified single nucleotide polymorphisms in HIV/AIDS cases associate with clinical parameters and disease progression

© 2016 Vladimir V. Anokhin et al.The genetic background of an individual plays an important role in the progression of HIV infection to AIDS. Identifying previously unknown or uncharacterized single nucleotide polymorphisms (SNPs) that associate with disease progression may reveal important therapeutic targets and provide a greater understanding of disease pathogenesis. In the present study, we employed ultra-high multiplex PCR on an Ion Torrent next-generation sequencing platform to sequence 23 innate immune genes from 94 individuals with HIV/AIDS. This data was used to identify potential associations of SNPs with clinical parameters and disease progression. SNPs that associated with an increased viral load were identified in the genes for the interleukin 15 receptor (IL15RA), toll-like receptor 7 (TLR7), tripartite motif-containing protein 5 (TRIM5), and two killer-cell immunoglobulin-like receptors (KIR2DL1 and KIR2DL3). Additionally, SNPs that associated with progression from HIV infection to AIDS were identified in two 2′-5′-oligoadenylate synthetase genes (OAS2 and OAS3). In contrast, other SNPs identified in OAS2 and OAS3 genes, as well as in the TRIM5 and KIR2DS4 genes, were associated with a slower progression of disease. Taken together, our data demonstrates the utility of ultra-high multiplex PCR in identifying polymorphisms of potential clinical significance and further,identifies SNPs that may play a role in HIV pathogenesis

Histological classification of mesial temporal sclerosis

Mesial temporal sclerosis (MTS) is the most common histopathology occurring in patients with drug-resistant temporal lobe epilepsy. Over the past decades, there have been various attempts to classify the variants of hippocampal neuronal cell loss in relation to postoperative outcome. However, no consensus on the common international definition and classification of MTS has been reached. The article describes the modern histological classification based on a semiquantitative hippocampal cell loss model. The publications dealing with the histological classification of mesial temporal sclerosis are reviewed

Gut-associated plasmacytoid dendritic cells display an immature phenotype and upregulated granzyme B in subjects with HIV/AIDS

© 2015 Boichuk, Khaiboullina, Ramazanov, Khasanova, Ivanovskaya, Nizamutdinov, Sharafutdinov, Martynova, DeMeirleir, Hulstaert, Anokhin, Rizvanov and Lombardi. Plasmacytoid dendritic cells (pDCs) in the periphery of subjects with human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) decrease over time, and the fate of these cells has been the subject of ongoing investigation. Previous studies using animal models as well as studies with humans suggest that these cells may redistribute to the gut. Other studies using animal models propose that the periphery pDCs are depleted and gut is repopulated with naive pDCs from the bone marrow. In the present study, we utilized immunohistochemistry to survey duodenum biopsies of subjects with HIV/AIDS and controls. We observed that subjects with HIV/AIDS had increased infiltration of Ki-67+/CD303+ pDCs, a phenotype consistent with bone marrow-derived pre-pDCs. In contrast, Ki-67+/CD303+ pDCs were not observed in control biopsies. We additionally observed that gut-associated pDCs in HIV/AIDS cases upregulate the proapoptotic enzyme granzyme B; however, no granzyme B was observed in the pDCs of control biopsies. Our data are consistent with reports in animal models that suggest periphery pDCs are depleted by exhaustion and that naive pDCs egress from the bone marrow and ultimately infiltrate the gut mucosa. Additionally, our observation of granzyme B upregulation in naive pDCs may identify a contributing factor to the gut pathology associated with HIV infection

Nitrogênio fixado simbioticamente por feijão‑caupi e gliricídia em sistemas tradicional e agroflorestal sob condições semiáridas

The objective of this work was to estimate the amounts of N fixed by cowpea in a traditional system and by cowpea and gliricidia in an agroforestry system in the Brazilian Northeast semiarid. The experiment was carried out in a randomized complete block design, in a split‑plot arrangement, with four replicates, in the semiarid region of the state of Paraíba, Brazil. Plots consisted of agroforestry and traditional systems (no trees), and split‑plots of the three crops planted between the tree rows in the agroforestry system. To estimate N fixation, plant samples were collected in the fourth growth cycle of the perennial species and in the fourth planting cycle of the annual species. In the agroforestry system with buffel grass and prickly‑pear cactus, gliricidia plants symbiotically fix high proportions of N (>50%) and contribute with higher N amounts (40 kg ha‑1 in leaves) than in the traditional system (11 kg ha‑1 in grain and 18 kg ha‑1 in straw). In the agroforestry system with maize and cowpea, gliricidia plants do not fix nitrogen, and N input is limited to the fixation by cowpea (2.7 kg ha‑1), which is lower than in the traditional system due to its lower biomass production.O objetivo deste trabalho foi estimar as quantidades de N fixadas em feijão‑caupi em sistema tradicional e em feijão‑caupi e gliricídia em sistema agroflorestal, sob condições semiáridas do Nordeste brasileiro. O experimento foi conduzido em blocos ao acaso, em arranjo em parcelas subdivididas, com quatro repetições, no semiárido da Paraíba. As parcelas consistiram dos sistemas agroflorestal e tradicional (sem árvores), e as subparcelas, dos três cultivos entre as linhas das árvores, no sistema agroflorestal. Para estimar a fixação de N, amostras das plantas foram coletadas no quarto ciclo de crescimento das espécies perenes e no quarto ciclo de cultivo das espécies anuais. No sistema agroflorestal com capim‑buffel e palma forrageira, a gliricídia fixa simbioticamente altas proporções de N (>50%) e adiciona quantidades maiores de N (40 kg ha‑1 nas folhas) que no sistema tradicional (11 kg ha‑1 nos grãos e 18 kg ha‑1 na palhada). No sistema agroflorestal com milho e feijão‑caupi, a gliricídia não fixa N e a adição de N é limitada à fixação no feijão‑caupi (2,7 kg ha‑1), que é menor que no sistema tradicional em razão da sua baixa produção de biomassa

FROM PERSONALIZED TO PRECISION MEDICINE

The need to maintain a high quality of life against a backdrop of its inevitably increasing duration is one of the main problems of modern health care. The concept of "right drug to the right patient at the right time", which at first was bearing the name "personalized", is currently unanimously approved by international scientific community as "precision medicine". Precision medicine takes all the individual characteristics into account: genes diversity, environment, lifestyles, and even bacterial microflora and also involves the use of the latest technological developments, which serves to ensure that each patient gets assistance fitting his state best. In the United States, Canada and France national precision medicine programs have already been submitted and implemented. The aim of this review is to describe the dynamic integration of precision medicine methods into routine medical practice and life of modern society. The new paradigm prospects description are complemented by figures, proving the already achieved success in the application of precise methods for example, the targeted therapy of cancer. All in all, the presence of real-life examples, proving the regularity of transition to a new paradigm, and a wide range  of technical and diagnostic capabilities available and constantly evolving make the all-round transition to precision medicine almost inevitable